Study of the regenerative capacity of the islet of Langerhans

1989 Fiscal Year Final Research Report Summary

• Project/Area Number: 61570641
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Kanazawa University
• Principal Investigator: YONEMURA Yutaka Lecturer, Surgery II, School of Medicine, Kanazawa University, 医学部, 講師 (20167042)
• Project Period (FY): 1986 – 1988
• Keywords: Parcreatectomy / diabetes mellitus / poly (ADP-ribose) synthetase inhibitor / regeneration of islet B cells / 膵B細胞の再生

Research Abstract

1.3-Aminobenzamide as poly(ADP-ribose) synthetase inhibitor was given to 90% depancreatized rats. The regenerative activities of B cells were examined in attempt to clarify the mechanism of preventing surgical diabetes. On the 30th day after pancreatectiay, the islets treated with saline were reduced and the number of B cells decreased. In deparcreatized and 3-aminobenzomide treated rat (AB), the islets were enlarged and hyperplasia of B cells was seen. Autoradiography and stuthwokinetic studies showed labeling and mitotic indices of B cells in AB had a higher level than those of saline group. These results suggest that poly(ADP-ribose) synthetuse inhibitor can induce self-replication of B cells in partially depancreated rats.
2. While the onset of diabetic state was observed in 82% of depancreatized rats without 3-aminobenzaamide, 29% of 3-aminobenzamide treated rats fell into diabetic state the morphologic examination of remnant pancreas revealed the islets from 3-AB-treated rats were enlarged. The percentage of labelled B cells reached a peak in the rats killed 3 and 5 days after operation, and then declined gradually, the results indicate that 3-AB may prevent diabetes mellitas in partially depancreatized rats by maintaining the function of B cells.