1988 Fiscal Year Final Research Report Summary
The mechanism with damage of liver cell membranes induced by abnormal oxygen conditions
| Project/Area Number |
61570736
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
麻酔学
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
YOSHIKUNI AMAKATA Shiga University of Medical Science, 医学部, 教授 (20028567)
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| Co-Investigator(Kenkyū-buntansha) |
HIROSHI KINUGAWA Shiga University of Medical Science, 医学部, 助手 (00177883)
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| Project Period (FY) |
1986 – 1988
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| Keywords | Isolated Hepatocyte / GOT / Immunohistochemistry / Morphology Cell Organelles / ミトコンドリア |
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| Research Abstract |
Effect of halothane on isolated rat's hepatocytes have investigated with morphologically and immunohistochemically indicated by GOT leakage. Cell damages were discussed by the decrement of microvilli using scanning electron microscope (SEM) and were checked by the changes of nucleus, mitochondria and the other cell organelles using transmission electron microscope (TEM). These were concerned with the halothane concentration.
The leakage of GOT, i.e., m-GOT plus s-GOT, was discussed by immunohistochemical staining. The decrement of s-GOT was dominantly even in the low concentration of halothane, on the other hand, m-GOT was resisted compared with s-GOT. But in the high concentration of halothane, both of them showed massive leakage of enzymes.
These results were discussed with TEM and was showed that-m-GOT existed in the cristae of mitochondria and s-GOT existed in the cytoplasm evenly.
Those investigators need to establish the route of leakage across the membrane of cell and mitochondria in further study.
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