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1987 Fiscal Year Final Research Report Summary

A study of micro-quantification of blood level of endotoxin in laboratory animals.

Research Project

Project/Area Number 61580041
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionOsaka University

Principal Investigator

KUROSAWA Tsutomu  Osaka University, Medical school, associate Professor, 医学部, 助教授 (60129997)

Project Period (FY) 1986 – 1987
KeywordsENDOTOXIN / MICRO-QUANTIFICATION / LABORATORY ANIMAL / 血液
Research Abstract

The aim of this project is the establishment of micro-quantificatin system of endotoxin in laboratory animals. Before the development of limulus test in which only ng/ml of endotoxin can be detected with the limulus lysate, in vivo endotoxin assay was only a method available to detect endotoxin.
In this project, PNA substrate method in which pg/ml of endotoxin can be quantified with limulus lysate and PNA substrate reagent was used. And also the suitable preparatin method of blood sample to be applied to this micro-quantificatin method was established using PCA in laboratory animals.
However the original PNA substrate method called Toxicolor test may not be used for the quantification of endotoxin in laboratory animals due to 1-3 <beta> glucan which activates Factor G and then causes false positive. The new PNA substrate method called Endospacy method, in which the Factor G wad excluded was testified and was proved to be applicable to quantify the blood level of endotoxin in various laboratory animals.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 黒澤努: 肝胆膵. 12(4). 579-585 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kodama.: Journalof Applied Bacteriology. 63. 225-260 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 黒澤努: "エンドトキシン臨床研究の新しい展開" 羊土社, 288 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Kurosawa: "Endotoxemia in Surgical Pafienfs." Liver Gallbladder and Pancreas. 12(4). 579-585 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Kodama.: "Quantitative measurement of endotoxin in rainbow trout (Salmo gairdneri) serum by the chromogenic substrate method." Journalof Applied Bacteriology. 63. 225-260 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KUROSAWA, Tsutomu: New scope of Clinical Endotoxin Research.Youdosya, Tokyo, 288 (1987)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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