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1987 Fiscal Year Final Research Report Summary

Developments of ultra-sensitive analysis of lipid oxidation and evaluation of antioxidant actibity by chemiluminescence

Research Project

Project/Area Number 61850150
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 有機工業化学
Research InstitutionUniversity of Tokyo

Principal Investigator

NIKI Etsuo  Univ. of Tokyo, Progessor, 工学部, 教授 (20011033)

Co-Investigator(Kenkyū-buntansha) TSUCHIYA Jyunichi  Univ. of Tokyo, Research Staff, 工学部, 助手 (40011051)
YAMAMOTO Yorihiro  Univ. of Tokyo, Research Staff, 工学部, 助手 (60134475)
Project Period (FY) 1986 – 1987
Keywordschemiluminescence / hydroperoxide / HPLC / lipid peroxide / 高速液体クロマトグラフィー
Research Abstract

The objectives of the present study were to develop a sensitive assay for determination of oxidation and also for evaluation of antioxidant activity. The thermal and redical-induced decomposition of hydroperoxides yieled chemiliminescence, but the quantitative analysis was difficult. The addition of sodium hypochlorite to a solution containing a small amount, of hydroperoxide oroduced a strong chemiluminescence. This method was quite sensitive and could be applied for assessing to oxidation deterioration of oils and fats.
Furthormore a new method for the detectrion of various lipid hydroperoxides and bydrogen preoxide at the picomole level has been developed by combining an HPLC system with an ultrasensitive analytical system besed on the detection of chemiluminescence emitted by isoluminol in the presence of hydroperoxide and microperoxidase. The addition of antioxidant to a chemiluminescence emitting medium, its intensity dectrased matkedly and after showing a minimum, it increased again to the initial level after some duration of time. The antioxidant activity could be evaluated from the above minimum intensity and duration of time. Thus, chemiluminescence method was found to be quick useful and applied.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 二木鋭雄: ペトロテック. 9. 583-587 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 二木鋭雄: ペトロテック. 9. 689-691 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E.Niki: J.Japan Petrol.Inst.30. 369-377 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Yamamoto: Anal.Biochem.160. 7-13 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Yamamoto: Biol.Med.3. 359-361 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本順寛,金田尚志,植田伸夫 編: "過酸化脂質実験法" 医歯薬出版, 245 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E. Niki: "Chemiluminescence - Basis and Application" PETROTECH. 9. 583-587 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E. Niki: "Autoxidation ad Studied by Chemiluminescence" RETROTECH. 9. 689-691 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E. Niki: "Study of Autoxidation by Chemfluminescence" J. Japan Petrol Inst.30. 369-377 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Yamamoto: "Detection and chetacerization of lipid hydeqperoxide at picomole levels by high-performance liquid chromatography" Anal, Biochem.160. 7-13 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Yamamoto: "Detection of hydroperoxides and hydrogen peroxide at picomole levels by an HPLC and isoluminol chemilumincescence assay" Biol. Med.3. 359-361 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Yamamoto: Ishiyakn Shuppan. Experiments for Lipid Peroxides, 238-241 (1987)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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