1988 Fiscal Year Final Research Report Summary
RECONSTRUCTION OF BIOMEMBRANE MOLECULES IN PLANAR LIPID BILAYER MEMBRANES
| Project/Area Number |
62304061
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | OSAKA UNIVERSITY |
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Principal Investigator |
TAKAGI MASAYUKI OSAKA UNIV. Associate Professor, 教養部, 助教授 (20028145)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI MICHIKI OSAKA UNIV. Professor, 基礎工学部, 教授 (40022595)
藤墳 規明 名古屋工業大学, 助教授 (60101268)
HOSHI TAKECHI UNIV. OF SHIZUOKA, Professor, 食品栄養科学部, 教授 (60004537)
SUGIYAMA HIROYUKI NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES, Associate Professor, 助教授 (20124224)
TSUDA MOTOYUKI SAPPORO MEDICAL COLLEGE, Assistant Professor, 講師 (60045458)
KIRINO YUTAKA KYUSHU UNIV. Professor
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| Project Period (FY) |
1987 – 1988
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| Keywords | Planar Lipid Bilayer Membrane / Ion Channels in Photoreceptor / Reconstruction of K^+-ehannel / 巨大ベシクル膜 / パッチング / アフリカツメガエルの卵母細胞 / グルタミン酸受容チャネル |
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| Research Abstract |
1) Identification and Purification of Ion Channels in Invertebrate (Octopus) Photoreceptor Membranes by Means of Planar Lipid Bilayer Membrane: The microvilli membrane of octopus photoreceptor were isolated, and fractionated into several protein components. Of these, two showed definite channel activities in record of their planar lipid bilayer membrane currents. Their conductances were 150 pS and 10 pS. The ion channel of 150 pS has no selectivity between K^+ and Na^+ and is permeable to the large organic cations, such as choline^+ and tetraethylammonium^+.
2) Reconstruction of K^+-channel in Planar Lipid Bllayer Membrane:Roughly purified K^+-channel proteins obtained from muscle sarcomic reticulum were reconstructed in a giant liposome of azolectin, which was patched with fine glass pipet ( =0.5um). An analysis on the single K^+-channel current on the patched membrane showed that the channel has a substate and the gating system is controlled by K^+ ion istelf.
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