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1989 Fiscal Year Final Research Report Summary

Cell kinetic analysis of malignant brain tumors by anti-bromodeowyuridine monoclonal antibody. Its relationship to clinical prognosis.

Research Project

Project/Area Number 62440058
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionTeikyo University

Principal Investigator

HOJO Shuntaro  Teikyo Univ. School of Med. Associate Prof., 医学部, 助教授 (70133072)

Co-Investigator(Kenkyū-buntansha) TSUJITA Yoshihiko  Teikyo Univ. School of Med. Assistant Prof., 医学部, 講師 (50163803)
TAMURA Akira  Teikyo Univ. School of Med. Professor, 医学部, 教授 (80111532)
SANO Keiji  Teikyo Univ. School of Med., 医学部, 客員教授 (20009880)
Project Period (FY) 1987 – 1989
KeywordsBUdR / Labeling indes (L.I.) / radiation therapy / cell kinetic analysis / 放射線療法
Research Abstract

BUdR (bromodeoxyuridine), incorporated in the nuclei of S-phase cells, was stained by immunohistochemical method. Labeling index (L.I.), the ratio of BUDR positive cells to the total number of the cells, was calculated in both surgical specimen and experimental neoplasm.
1) LIs of gliomas spread in a wide range. But they correlated with clinical malignancy and histopathological classification. Mean reccurence-free period of the patients with L.I. over 5% was 9 months and that of the patients with L.I. between 3 to 5% was 14.5 months. On the other hand, median survival of the patient with L.I. over 5% and between 3 to 5% were 16.5 months and 33.5 months respectively. There was significant difference in LIs of each subtypes of astrocytic neoplasm. Mean LIs of glioblastoma, anaplastic astrocytoma and well differentiated astrocytoma were 8.5%, 4.2% and 1.2% respectively.
2) On histological observation, distribution of S-phase cells in the tumor mass was not homogenous. They clustered around the blood vessels and at the margin of a tumor. In addition to the neoplastic cells, some stroma cells, such as endotherial cells, fibroblasts and infiltrated lymphcells, were also stained positively.
3) Radiation therapy reduced BUDR uptake in glioblastoma cells. LI decreased from 8.2% to 3.8% after irradiation.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Matsutani M,Kohno T,Nagashima T, Nagayam I,,Matsuda T,Hoshino T,Sano: "Clinical trail of intravenous infusion of bromodeoxyuridine(BUdR)for radiosensitization of malignant brain tumors." Radiation Medicine. 6. 33-39 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松谷雅生、中村治: "乳癌脳転移の病態とquality of life ー肺癌脳転移治療経験よりの考察" 乳癌の臨床. 3. 531-521 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi Y,Koike M,Matsutani T,Nagayama I,Matsuda T,Hoshino T,Sano K.: "Effects of fixation time and enzymatic digestion on immunohistochemical demonstration of bromodeoxyuridine in formalin-fixed." Paraffin-embedded tissue. 36. 511-514 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松谷雅生、中村治、長島正、植木敬介、中村正直: "Glioblastomaの手術と治療予後" 癌の臨床. 35. 1227-1232 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka K,Sato C,Maeda Y,Koike M,Matsutani M,Yamada K,Miyaki M.: "Establishment of a human malignant meningioma cell line with amplified c-cmyc oncogene." Cancer. 64. 2243-2249 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sano K,Matsutani M,Seto T.: "So-called intracranial germ cell tumours:personal experiences and a theory of their pathogenesis." Neurological Research. 11. 118-126 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐野圭司:glioma治療の原則: "gliomaのすべて" 鈴木二郎編 にゅ-ろん社, (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsutani M, Kohno T, Nagashima T, Nagayam I, Matsuda T, Hoshino T, Sano K.: "Clinical trail of intravenous infusion of bromodeoxyuridine (BUdR) for radiosensitization of malignant brain tumor" Radiation Medicine. 6. 33-39 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi Y, Koike M, Matsutani T, Hoshino T.: "Effects of fixation time and enzymatic digestion on immunohistochemical demonstration of bromodeoxyuridine in formalin-fixed." Paraffin-embedded tissue 36:511-514, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Asai A, Matsutani M, Kohmo T, Nakamura O, Tanaka H, Fujimaki T, Funada N, Matsuda T, Nagata K, Takakura K.: "Subacute brain atrophy after radiation therapy for malignant brain tumor" Cancer 63:1969-1972, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka K, Sato C, Maeda Y, Koike M, Matsutani M, Yamada k, Miyake M.: "Establishment of a human malignant meningioma cell line with amplified c-cmyc oncogene." Cancer 64:2243-2249, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sano K, Matsutani M, Seto T.: "So-called intracranial germ cell tumors: personal experiences and a theory of their pathogenesis." Neurological Research 11:118-126, 1989.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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