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1989 Fiscal Year Final Research Report Summary

Regulation of animal cell differentiation by food constituent.

Research Project

Project/Area Number 62470126
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionKyoto University (1988-1989)
Nagoya University (1987)

Principal Investigator

SUGIMOTO Etsuro  Kyoto Univ., Fac. of Agric., Professor, 農学部, 教授 (50026522)

Co-Investigator(Kenkyū-buntansha) HIGUCHI Masako  Kyoto Univ., Fac. of Agric., Research Associate, 農学部, 教務職員 (40110762)
KAWADA Teruo  Kyoto Univ., Fac. of Agric., Instructor, 農学部, 助手 (10177701)
FUSHIKI Tohru  Kyoto Univ., Fac. of Agric., Associate Professor, 農学部, 助教授 (20135544)
Project Period (FY) 1987 – 1989
Keywordsfood constituent / differentiation / cell proliferation / cell culture / intestinal epithelial cell / adipocyte / vitamins
Research Abstract

In order to clarify factors and their function to differentiation, two cell lines have been selected as model systems.
It was shown that IEC-18 cells were model of stem cell of intestinal epithelial differentiation. A factor which may induce differentiation of intestinal epithelial cell lines in vitro was found in an acid extract of adult rat small intestine. The addition of a partially purified acetic acid extract of rat small intestine to IEC-18 cell culture dishes increased sucrase activity within 48 hrs. Thymidine incorporation markedly decreased within 24 hrs. Significant development of microvilli-like structures was observed on the acid extract-treated IEC-18 cells, compared with controls. This activity of tat acid extract was heat-stable and the apparent molecular weight of the factor was 400-800. These findings suggested that the factor may be related to the epithelial differentiation of rat small intestinal crypt cells.
Murine fibroblast 3T3-L1 cells differentiate from preadipocytes to adipocytes in culture. The effects of 20 kinds of vitamins or their analogues on the growth rate of preadipocytes and the terminal differentiation of preadipocytes to adipocytes were systematically compared in 3T3-L1 cells; The addition of vitamin C markedly increased the growth rate of preadipocytes at over 50muM. The addition of vitamin K_3 slowed down the growth rate at over 0.1muM. In water soluble vitamins and their analogues tested, the vitamin B_6 group and vitamin C significantly stimulated the differentiation, and consequently increased the glycerophosphate dehydrogenase activity and triglyceride accumulation, to a concentration over 10muM. "Many fat"soluble vitamins and their analogues (the vitamin A group, including beta-carotene, the vitamin D group, vitamin E and vitamin K group) strongly inhibited the adipose conversion of 3T3-L1 cells at muM level.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y.Aratani: "Lithium ion reversibly inhibits inducer-stimulated adipose conversion of 3T3-L1 cells." FEBS Lett.218. 47-51 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Miki: "Reversible interconversion between primitive endoerm-and parietal endoderm like F9 cells demostrated by mRNA expression." J.Biochem.102. 385-392 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Fukuoka: "Competition of a growth stimulating/cholecystokinin(cck)-releasing peptide(monitor peptide)with epidermal growth factor for binding to 3T3 fibroblasts." Biochem.Biophys.Res.Commun.145. 646-650 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.shintani: "Differentiation of intestinal epithelial cell line(IEC-18)by an acid extract of rat small intestine." FEBS Lett.255. 423-426 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kawada: "Comparative investigation of vitamins and their analogues on terminal differentiation,from preadipocytes to adipocytes,of 3T3-L1 cells," Com.Biochem.Biophys.印刷中.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kawada: "Proliferation of 3T3-L1 preadipocytes in completely defined serum-free medium." 投稿中.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Aratani: "Lithium ion reversibly inhibits inducerstimulated adipose conversion of 3T3-L1 cells." FEBS Lett., 218, 47-51, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Miki: "Reversible interconversion between primitive endoderm- and parietal endoderm like F9 cells demonstrated by mRNA expression." J. Biochem., 102, 385-392, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Fukuoka: "Competition of a growth stimulating/cholecystokinin (cck)-releaing peptide (monitor peptide) with epidermal growth factor for binding to 3T3 fibroblasts." Biochem. Biophys. Res. Commun., 145, 646-650, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Shintani: "Differentiation of intestinal epithelial cell line (IEC-18) by an acid extract of rat small intestine." FEBS Lett., 255, 423-426, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Kawada: "Comparative investigation of vitamins and their analogues on terminal differentiation, from preadipocytes to adipocytes, of 3T3-L1 cells." Com. Biochem. Biophys.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Kawada: "Proliferation of 3T3-L1 preadipocytes in completely defined serum-free medium."

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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