1989 Fiscal Year Final Research Report Summary
Molecular Analysis of Structural Changes of Plant Nuclear DNA.
| Project/Area Number |
62480007
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
植物生理学
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
TANIFUJI Shigeyuki Hokkaido Univ., Fac. of Sci., Professor, 理学部, 教授 (50000774)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KATO Atsushi Hokkaido Univ., Fac. of Sci., Lecturer, 理学部, 講師 (90177428)
|
|---|
| Project Period (FY) |
1987 – 1989
|
| Keywords | Satellite DNA / 5.8S rRNA gene / Intergenic spacer of rDNA / Imitation-Methionine tRNA gene / Mutated histidine tRNA gene / Arabidopsis thaliana / Vicia faba / Pisum sativum |
|---|
| Research Abstract |
For an understanding of structural differentiation of plant nuclear genomes, we analyzed molecular structure of highly repeated DNA sequences, rRNA genes and tRNA genes in carrot (Daucus carota), broad bean (Vicia faba), pea (Pisum sativum) and Arabidopsis thaliana.
Our previous studies-have shown that when carrot root disks were cultured in the presence of auxin, certain portions of cells initiated DNA replication synchronously, and a GC-rich repeated DNA component began to replicate earlier than the main band DNA. By the use of benzoylated naphthoylated DEAE-cellulose column chromatography, we obtained evidence that this early replicating DNA remained at the state of the double-stranded molecules containing substantial single-stranded regions for much longer period than the main DNA component.
We determined the nucleotide sequence of the 18S rRNA coding region of broad bean rRNA gene.
We made a phylogenetic tree of 5.8S rRNA sequences of some representative eukaryotes including broad bean and carrot plants with which the sequences of their genes were determined by us.
The complete nucleotide sequence of the intergenic spacer (IGS) of rRNA gene was determined for broad bean and pea plants. This spacer region showed a considerable sequence similarity between these two legume plants. However, the patterns of molecular organization were different from each other. The pea IGS contained only one type of repeated sequence, whereas the broad bean IGS contained five kinds of repeated sequences. The sequence of 17 bp surrounding the imitation site of transcription which was tentatively determined by Sl mapping experiment was identical between broad bean and pea plants, and also similar, though not identical, to the corresponding regions of maize and wheat rRNA genes.
The nucleotide sequences of a gene for methionine-initiation tRNA and a mutated gene for histidine tRNA of Arabidopsis thaliana were also determined.
|
