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1988 Fiscal Year Final Research Report Summary

Studies on Host Recognition Mechanisms in Symbiotic Nitrogen-fixing System

Research Project

Project/Area Number 62480054
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionThe University of Tokyo

Principal Investigator

MARUYAMA Yoshiharu  Faculty of Agriculture, The University of Tokyo, 農学部, 教授 (60011863)

Project Period (FY) 1987 – 1988
KeywordsSymbiotic nitrogen fixation / Host specificity / Lectin / Leghemoglobin / Non-nodulating soybean / Astragalus sinicus L. / 熱帯性マメ科植物
Research Abstract

(1) Host specificity of Rhizobium sp. Astragalus sinicus L. the rhizibium showed high host specificity against Astragalus sinicus L. by cross inoculation test. Lectin was isolated from the seed of Astragalus and tested the reaction with various other root nodule bacteria. The lectin showed the reactivity only with Rhizobium sp. Astragalus. The lectin was purified about 30 times of specific activity by ammonium sulfate fractionation, DEAE- and CM- cellulose column chromatography. Mega-plasmids were isolated from R. sp. Kohnosu and R. sp. Nagano of Astragalus and named pRko 1, pRko 2, pRna 1 and pRna 2. Their molecular weight was 72.7 201,112 and 293 Md. pRko 1 was hybridized with nif D.
(2) Non-nodulating strain of soybean (rj 1). The difference of lectin of root between ri 1 and wild strain (Rj 1) was examined by using antilectin antibody. No difference was observed. PAGE pattern of proteins extracted from roots was not also any significant differences between rj 1 and Rj 1.
(3) Symbiotic system in some tropical cultivated legumes. Root nodule bacteria were isolated from Vigna sinensis, Psophocarpus tetragonolobus and Vigna umbelleta and their chracters, host specificity, nitrogenfixing activity were examind. Leghemoglobins were also investigated.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 石井忠雄: 日本土壌肥料学雑誌. 58. 7-11 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] W.Y.Shieh: Can.J.Microbiol.34. 886-890 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Sekiguchi: J.Basic Microbiol.28. 529-539 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] F.Tagawa: Plant and Cell Physiol.(1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.M.Merestela: Agric.Biol.Chem.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Ishii: "Estimation of nodulation by inoculum strain with marker strain method." Jap. J. Soil Science and Plant Nutr.58. 7-11 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] W. Y. Shieh: "New marine nitrogen-fixing bacteria isolated from an eelgrass (Zostera marina) bed." Can. J. Microbiol.34. 886-890 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Sekiguchi: "Assimilatory reduction of nitrate in Rhizobium meliloti." J. Basic Microbiol.28. 529-539 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F. Tagawa: "Characterization of non-nodulating soybean." Plant and Cell Physiol.(1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. M. Merestela: "Symbiotic nitrogen fixation by some tropical cultivated legumes." Agric. Biol. Chem.(1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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