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1989 Fiscal Year Final Research Report Summary

Parallel and convergent neuronal connections and neuroactive substances

Research Project

Project/Area Number 62480098
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIZUNO Noboru  Kyoto Univ., Fac. Med., Professor, 医学部, 教授 (10025596)

Co-Investigator(Kenkyū-buntansha) KONISHI Akira  Kyoto Univ., Sch. Med., Technol., Prof., 医療技術短期大学部, 教授 (00025660)
KANEKO Takeshi  Kyoto Univ., Fac. Med., Res. Assoc., 医学部, 助手 (90177519)
YASUI Yukihiko  Kyoto Univ., Fac. Med., Res. Assoc., 医学部, 助手 (30174501)
SUGIMOTO Tetsuo  Kyoto Univ., Fac. Med., Lecturer, 医学部, 講師 (90144352)
ITOH Kazuo  Kyoto Univ., Fac. Med., Assoc. Prof., 医学部, 助教授 (60093184)
Project Period (FY) 1987 – 1989
KeywordsGlutaminase / Enkephalin (ENK) / Substance P (SP) / Glutamate / Catecholamine / Cholecystokinin (CCK) / Serotonin / Vasoactive intestinal polypeptide (VIP)
Research Abstract

1. Distribution of putative glutamatergic neurons in the cerbral cortex, thalamus, lower brainstem and cerebellum of the rat was examined immunohistochemically by using a monoclonal antibody against phosphate-activated glutaminase (PAG), which has been proposed to be a major synthetic enzyme of transmitter glutamate. Intensely-stained ueuronal cell bodies were seen in the pyramidal cells in layers V and VI in the neocortex and mesocortex, and pyramidal cells in Ammon's horn, neurons in the main precerebellar nuclei sending mossy-fibers to the cerebellum. PAG was also shown in many monoaminergic neurons by a double-staining method using the anti-glutaminase antibody combined with an antiserum against TH, DBH,PNMT or serotonin.
2. Convergence of serotonin (5HT)-, enkephalin (ENK)- and substance P (SP)-like immuno reactive (LI) fibers on single pudendal motoneurons of Onuf's nucleus was shown by retrogradely labeling neurons in Onuf's nucleus with HRP, and then by immunohistochemically lab … More eling 5HT-, ENK- and SP-fibers in the same sections using distinct chromogen. Similar studies were also done on the medullary dorsal horn, mesencephalic trigeminal nucleus, and lumbar spinal cord.
3. Cholecystokinin (CCK)- and/or vasoactive intestinal polypeptide (VIP)-LI was shown in many ceurons in the thalmic VL neurons which were retrogradely labeled with WGA-HRP injected into the frontal cortex of the rat. VIP- or CCK-LI was observed in 70-80% or 50-60% of thalamocortical VL neurons, respectively. Almost all of thalamocortical VL neurons with CCK-LI exhibited VIP-LI.
4. Many fibers with calcitonin gene-related peptide (CGRP)-LI in the cerebellum of the cat were demonstrated to arise from the pontine nuclei: when a lesion was placed in the pontine prachium, mossy fiber endings with CGRP-LI were reduced in number in the cerebellar hemis phere of the posterior lobe ipsilateral to the lesion. Fine varicose fibers with CGRP-LI within the dentate nucleus were also decreased in number ipsilateral to the lesion.
5. A combined method of the tracing of WGA-HRP and the immunohistochemistry of substance P revealed that many SP-LU neurons in the supramammillary nucleus of cat hypothalamus sent their axons to the hippocampal formation: SP-LI axons in the hippocampal formation abd ento rhinal cortex were markedly resuced in number ipilaterally after placing an electrothermic lesion in the supramammillary region of the hypothalamus. Less

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Mizuno,Noboru: "Direct projections from the subthalamic nucleus of Luys to the spinal cord in the Japanese monkey" Neuroscience Letters. 89. 13-18 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamiya,Hiroto: "A somatosensory and auditory relay nucleus in the rostral part of the ventrolateral medulla:a mophological study in the cat" Journal of Comparative Neurology. 273. 421-435 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasui,Yukihiko: "The cingulate gyrus of the cat receives projection fibers from the thalamic region ventral to the ventral border of the ventrobasal complex in the cat" Journal of Comparative Neurology. 274. 91-100 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ino,Tadashi: "Direct projections from Ammon's horn to the rostral raphe regions in the brainstem of the cat" Brain Research. 479. 157-161 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tashiro,Takashi: "Convergence of serotonin-,enkephalin-and substance P-like immunoreactive afferent fibers on single pudendal motoneurons in the Onuf's nucleus of the cat" Brain Research. 481. 392-398 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogawa,Reiko: "Co-existence of vasoactive intestinal polypeptide(VIP)-and cholecystokinin(CCK)-like immunoreactivities in thalamocortical neuron in the ventrolateral nucleus of the rat" Brain Research. 490. 152-156 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko, Takeshi: "An immunohistochemical study of glutaminase-containing neurons in the cerebral cortex and thalamus of the rat" J. Comp. Neurol., 267: 590-602, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugimoto, Tetsuo: "Calcitonin gene-related peptide-like immunoreactivity in neuronal elements of the cat cerebellum" Brain Res., 439: 147-154, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashiro, Takashi: "Distribution of axons exhibiting both enkephalin- and serotonin-like immunoreactivities in the lumbar cord segments: an immunohistochemical study in the cat" Brain Res., 440: 357-362, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizuno, Noboru: "Direct projections from the subthalamic nucleus of Luys to the spinal cord in the Japanese monkey" Neurosci. Lett., 89: 13-18, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamiya, Hiroto: "A somatosensory and auditory relay nucleus in the rostral part of the ventrolateral medulla: a morphological study in the cat" J. Comp. Neurol., 272: 421-435, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasui, Yukihiko: "The cingulate gyrus of the cat receives projection fibers from the thalamic region ventral to the ventral to the ventral border of the ventrobasal complex in the cat" J. Comp. Neurol., 274: 91-100, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ino, Tadashi: "The supramammillary region of the cat sends substace P-like immunoreactive axons to the hippocamal formation and the entorhinal cortex" Neurosci. Lett., 90: 259-264, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko, Takeshi: "Correlation between immunochemical characteristics and immunohistochemical applicability of nine lines of monoclonal antibodies against rat brain glutaminase" J. Histochem. Cytochem., 36: 997-1004, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ino, Tadashi: "Direct projections of non-pyramidal neurons of Ammon's horn to the supramammillary region in the cat" Brain Res., 460: 173-177, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ino, Tadashi: "Direct projections from Ammon's horn to the rostral raphe regions in the brainstem of the cat" Brain Res., 479: 157-161, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashiro, Takashi: "Convergence of serotonin-, enkephalin- and substance P-like immunoreactive afferent fibers on single pudendal motoneurons in the Onuf's nucleus of the cat: A light microscope study by the triple immunocytochemical staining technique combined with the retrograde HRP-tracing method" Brain Res., 481: 392-398, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa, Reiko: "Co-existence of vasoactive intestinal polypeptide (VIP)- and cholecytokinin (CCK)-like immunoreactivities in thalamocortical neuron in the ventrolateral nucleus of the rat" Brain Res., 490: 152-156, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashiro, Takashi: "Convergence of serotonin-, enkephalin- and substance P-like immunoreactive afferent fibers onto cat medullary dorsal horn projection neurons: a triple immunocytochemical staining technique combined with the retrograde HRP-tracing method." Brain Res., 491: 360-365, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashiro, Takashi: "Enkephalin-, substance P- and serotonin-like immunoreactive axonal varicosities in close apposition to perikarya of mesencephalic trigeminal nucleus in the cat" Brain Res., 494: 162-167, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashiro, Takashi: "Possible origins of substance P-like immunoreactive axons within Onuf's nucleus of the cat" Brain Res., 497: 177-182, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko, Takeshi: "Glutaminase-like immunoreactivity in the lower brainstem and cerebellum of the adult rat" Neuroscience, 32: 79-98, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko, Takeshi: "Immunohistochemical demonstration of glutaminase in catecholaminergic and seroninergic neurons of rat brain" Brain Res., 507: 151-154.1990.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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