1988 Fiscal Year Final Research Report Summary
Molecular cloning of human elastin cDNA and its application to heveditary elastin disease
| Project/Area Number |
62480234
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Keio University |
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Principal Investigator |
TAJIMA Shingo Keio University School of Medicine, assist. Prof, 医学部・皮膚科, 講師 (60129525)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAOKA Koichi Keio University School of Medicine, 医学部・皮膚科, 助手 (70170652)
MIYAKAWA Shun-Ichi Keio University School of Medicine, 医学部・皮膚科, 助手 (10146623)
FUKASAWA Toshio Keio University School of Medicine, Prof., 医学部・分子遺伝, 教授 (90029934)
NISHIKAWA Takeji Keio University School of Medicine, Prof., 医学部・皮膚科, 教授 (50051579)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Elastin mRNA / Skin fibroblasts / Anetoderma |
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| Research Abstract |
We have investigated the elastin metabolism in anetoderma patient using human elastin cDNA as a probe. The patient showed multiple atrophic macules in the skin which lack elastic fibers on the histochemical observation. Assay of crude elastin content in the skin showed a remarkable reduction in the patients skin the fibroblasts from the patient and normal control skin were grown and RNA was isolated by 6M SH-Hcl and analyzed by northern blot analysis, using a human elastin cdna as a probe. No difference of elastin mRNA content between the patient and control was found. The result indicated that the metabolic defect in this disease is in the post-translational step of elastin synthesis, not in the transcriptional step.
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