1989 Fiscal Year Final Research Report Summary
Studies on Treatment of Diabetes by Transplantation of Cryopreserved Langerhans islets of Pancreas
| Project/Area Number |
62480272
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | FUKUSHIMA MEDICAL COLLEGE |
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Principal Investigator |
MOTOKI Ryoichi Fukushima Medical College, School of Medicine, Professor, 医学部, 教授 (90045617)
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| Co-Investigator(Kenkyū-buntansha) |
KAMEDA Toshio Fukushima Medical College, School of Medicine, 医学部, 副手
MUTO Atsushi Fukushima Medical College, School of Medicine, 医学部, 副手
ASANO Hiroshi Fukushima Medical College, School of Medicine, 医学部, 副手
MIURA Junichi Fukushima Medical College, School of Medicine, 医学部, 助手 (60209715)
INOUE Hitoshi Fukushima Medical College, School of Medicine, Associate Professor., 医学部, 助教授 (90045782)
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| Project Period (FY) |
1987 – 1989
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| Keywords | Pancreatic Transplantation / Islets Transplant / Cryopreservation / Diabetes |
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| Research Abstract |
The aim of these studies was to evaluate the viability of cryopreserved islets of Langerhans in vitro and in vivo. Islets were isolated from rats by collagenase digestion and Ficoll purification, cooled slowly a a rate of 0.25ﾟC/min or rapidly at 25ﾟC/min to -40ﾟC and stored at -196ﾟC in liquid nitrogen. Frozen islets were thawed and cultured for 24 hours. In the in vitro study, cultured islets were assessed for viability by their dynamic response to glucose challenge during perofisopn. In the in vivo study, cultured islets were transplanted into the intravenous glucose tolerance test ( ivGTT ) and histological study. The results obtained were: (1) The yield of islets after collagenase digestion was 594.3<plus-minus> 103.7 per pancreas , and after Ficoll purification , 509<plus-minus>79.7 (85.5<plus-minus> 3.5%. (2) In freslily-isolated islets, insulin secretion was bipliasic, and itreturiled to the baseline value. (3)Frozen-thawed islets showed an abnormal response to glucose, but it was reversible under appropriate culture conditions. (4) Insulin secretion was slightly low and in rapidly cooled islets, but their dynamic response was similar to that of slowly cooled islets. (5) Transplantation produced normoglycemia in diabetic rats. (6) lntravenous glucose tolerance tests showed good K-value of 1.49 % /min which was 2.18 % /mon in freshly isolated i
These results indicate that the perifusion method is useful in evaluating the vitality of cryopreserved islets of Langerhans.
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