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1988 Fiscal Year Final Research Report Summary

The Effect of Eluate from Composite Resin on Lymphoid cells.

Research Project

Project/Area Number 62480386
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

TORII Mitsuo  Osaka University Faculty of Dentistry Associate Professor, 歯学部, 助教授 (30116066)

Co-Investigator(Kenkyū-buntansha) YASUNAGA Tetsuya  Osaka University Faculty of Dentistry Research Assistant, 歯学部, 助手 (70182342)
TSUCHITANI Yasuhiko  Osaka University Faculty of Dentistry Professor, 歯学部, 教授 (40028709)
Project Period (FY) 1987 – 1988
KeywordsComposite resin / Eluate / マイトジェン活性
Research Abstract

The mitogenic activity of eluate from a hardened composite resin (Photoclearfil A) for spleen cells derived from BALB/c mouse was investigated by using tritiated thymidine uptake. Resin discs (10 mm in diameter, 2mm in thickness) were immersed in RPMI-1640 medium (1 disc/5 ml) and the eluate was serially diluted by two fold. Dose-response was not established because the dispersion of data was very wide but both finally two and 16-fold diluted samples always showed clear mitogenic activity.
It was attempted to specify the mitogenic substances which were included in the eluate from composite resin. The eluate to distilled water was fructionated by using high performance liquid chromatography (HPLC). Unreacted monomers Bis-GMA and 3G were confirmed to be included. Many other peaks were observed but we could not qualify them. Mitogenic activity of Bis-GMA and 3G were investigated. Both of these two materials induced good mitogenic responses on BALB/c mouse spleen cells.
It was sugested that some of the mitogenic activity of eluate from composite resin depended on unreacted monomers Bis-GMA and 3G.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 鳥居光男: 日本歯科保存学雑誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuo, Torii: "The Mitogenic Activity of Eluate from Composite Resin." The Japanese Journal of Conservative Dentistry.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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