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1989 Fiscal Year Final Research Report Summary

Studies on vaccine for laboratory animals using anti-idiotype antibodies

Research Project

Project/Area Number 62480440
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionTeikyo University

Principal Investigator

KUHARA Takatoshi  Teikyo U. Sch. Med., Assistant professor, 医学部, 講師 (70134616)

Co-Investigator(Kenkyū-buntansha) TAJIMA Tomoko  Teikyo U. Sch. Med., Research associate, 医学部, 助手 (90173145)
ISHIGURO Toshikazu  Teikyo U. Sch. Med., Research associate, 医学部, 助手 (30082275)
TAMURA Hiroshi  Teikyo U. Sch. Med., Associate professor, 医学部, 助教授 (30101728)
MAEJIMA Kazuyoshi  Keio U. Sch. Med., Professor, 医学部, 教授 (70051464)
ABE Yoshiaki  Teikyo U. Sch. Med., Professor, 医学部, 教授 (20089296)
Project Period (FY) 1987 – 1989
KeywordsMycoplasma / Sendai virus / Monoclonal antibodies / Antiidiotype antibodies
Research Abstract

Results of the Project
1.Both humoral and cell-mediated immune responses were depressed in Mycoplasma Pulmonis ( M. pulmonis )-infected DBA/2 mice 2 to 6 weeks after infection.
2.Antibody responses to sheep red blood cells were augmented in M. pulmonis-infected athymic BALB/C nude( nu/nu ) mice 1 to 4 weeks after infection.
3.About 30 hybridoma clones which produce monoclonal antibodies to M. pulmonis were obtained.
4.Among the monoclonal antibodies to M. pulmonis, 2 monoclonal antibodies were found to have the ability to inhibit slightly the formation of lung lesions caused by M. pulmonis infection.
5.Sera obtained from M. pulmonis-infected mice were found to have the ability to inhibit significantly the formation of lung lesions caused by M. pulmonis infection.
6.About 15 hybridoma clones which produce monoclonal antibodies to Sendai virus ( HVJ were obtained. Among the monoclonal antibodies to Sendai virus, none of them were found to have the ability to protect animals from Sendai virus infection.
7.Many hybridomas which produce antibodies to streptococcal erythrogenic toxin were obtained, but the cloning of the hybridomas has not yet been performed.
Future Aspects of the Project
1.Monoclonal antibodies to various pathogenic agents in laboratory animals will be produced. Anti-idiotype antibodies (preferably monoclonal anti-idiotype antibodies) to those monoclonal antibodies will be produced and applied as vaccine.
2.Hybridoma clones which produce monoclonal antibodies to streptococcal erythrogenic toxin will be produced.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 田中裕士 他: "マウスmycoplasma pulmonis肺炎の治療に関する検討-抗生剤とprednisoloneの投薬時期について-" マイコプラズマ研究. 16. 76-79 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中裕士 他: "マウスmycoplasma pulmonis肺炎における抗生剤、免疫抑制剤および免疫調節剤の治療効果に関する研究" 札幌医学雑誌. 58. 403-416 (1989)

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      「研究成果報告書概要(和文)」より
  • [Publications] 久原孝俊 他: "免疫研究と実験動物-とくに免疫不全動物について" 病態生理. 8. 199-208 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 久原孝俊 他: "modification of immune responses in mice experimentally infected with mycoplasma pulmonis" Infection and Immunity(投稿中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 久原孝俊 他: "Production and characterization of monoclonal antibodies to mycoplasma pulmonis" Journal of Immunology(投稿準備中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 久原孝俊 他: "Effect of serum thymic factor(FTS)on mycoplasma pulmonis infection in mice" Journal of Immunology(投稿準備中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 久原孝俊 他(分担執筆): "免疫学的性状(「実験動物の生物学的特性デ-タ」監修:田嶋嘉雄、編集:堀内茂友、輿水馨)" ソフトサイエンス社, 656 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 久原孝俊(分担執筆): "マウス白血病、マウス乳癌、自然発生腫瘍(「獣医実験動物学」編集:光岡知足、波岡茂郎、輿水馨、前島一淑,(印刷中)" 川島書店, (1990)

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      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka, Hiroshi et al.: "Studies on treatment of Mycoplasma pulmonis pneumonia in mice - Timing of administration of antibiotics and prednisolone - (in Japanese)" Proc. of the XVI Ann. Cong. of the Japn. Soc. of Mycoplasmology 16:76-79, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, Hiroshi et al.: "Studies on the effect of antibiotics, immunosuppressive agents, and immunomodulating agents on treatment of Mycoplasma pulmonis pneumonia in mice ( in Japanese )" The Sapporo Medical Journal 58:403-416, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuhara, Takatoshi et al.: "The use of laboratory animals in immunological studies - With special reference to immunodeficient animals ( in Japanese )" Medicina Philosophica 8:199-208, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuhara, Takatoshi et al.: "Modulation of immune responses in mice experimentally infected with Mycoplasma pulmonis" Infection and Immunity.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuhara, Takatoshi et al.: "Production and characterization of monoclonal antibodies to Mycoplasma pulmonis" Journal of Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuhara, Takatoshi et al.: "Effect of serum thymic factor ( FTS ) on Mycoplasma pulmonis infection in mice" Journal of Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuhara, Takatoshi et al.: "Immunological characteristics in "Biological Data of Laboratory Animals" (eds., S. Horiuchi and K. Koshimizu ) Soft Science, Ltd." pp. 656 1989.

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kuhara, Takatoshi et al.: "Murine leukemia, Mouse mammary tumor, Spontaneously occurring tumors in "Veterinary Laboratory Animal Science" (eds., T. Mitsuoka, S. Namioka, K. Koshimuzu, and K. Maejima ) Kawashima Shoten, Ltd." 1990.

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      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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