1988 Fiscal Year Final Research Report Summary
Microangiographic Study on the relationship between Blood Flow Pattern and Cardiovascular Morphogenesis in the Early Chick Embryo
| Project/Area Number |
62570443
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
NISHIBATAKE Makoto Associate, Tokyo Women's Medical College, 医学部, 助手 (90192252)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Hitoshi Associate, Tokyo Women's Medical College (90201657)
IKEDA Kazuo Associate, Tokyo Women's Medical College (10168122)
TAKAOI Atsuyoshi Professor, Tokyo Women's Medical College (70075167)
NAKAZAWA Makoto Associate Professor, Tokyo Women's Medical College (10075567)
TOMITA Sachiko Associate, Tokyo Women's Medical College (10159171)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Embryonic blood flow pattern / Flow molding theory / Laminar flow / Spiral blood flow Embryonic Cardiac Contraction pattern / 胎生期心収縮パターン / 弁様機能 |
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| Research Abstract |
It has been generally accepted that early embryonic hemodynamics greatly contribute to the cardiovascular morphogenesis. Using microangiography in the chick embryo, we studied blood flow pattern in the early embryonic stages. In the first year of the project, intracardiac streams of blue dye injected into peripheral vitelline veins were recorded by 35mm microscopic camera on the color slide films. Multiple laminar flows, which made clockwise spiral fashion in their direction, were recognized before septation of the outflow tract. In the later stages, those multiple spiral flows increased the degree of spirals and tended to be divided into two major flows, right and left heart flow, in parallel with the development of truncoconal ridges. In the second year of the project, high-speed video recording system was applied to confirm the results obtained during the first year. Moreover, we analyzed the relationship between cardiac cycle and cardiac contraction pattern. We also analyzed how the presumptive atrioventricular and semilunar valve regions establish their valve-like function in the early stages. As a model for abnormal cardiovascular morphogenesis, cardiac neural crest-ablated embryos were used in this microangiographic study because preliminary study induced high incidence of cardiovascular malformations with similar procedure.
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Research Products
(9 results)
