Biological and biochemical analysis of autocrine growth factor produced by a cell line of human malignant astrocytoma (TM-1)

1989 Fiscal Year Final Research Report Summary

• Project/Area Number: 62570650
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Toyama Medical and Pharmaceutical University
• Principal Investigator: OKA Nobuo Toyama Medical and Pharmaceutical University, Department of Neurosurgery, Assistant Professor, 附属病院, 講師 (10135006)
• Co-Investigator(Kenkyū-buntansha): ARAI Kenji Toyama Medical and Pharmaceutical University, Department of Neurosurgery, Resear, 附属病院, 助手 (90159476) ; NOGAMI Kanehito Toyama Medical and Pharmaceutical University, Department of Neurosurgery, Resear, 医学部, 助手 (90208306) ; KURIMOTO Masanori Toyama Medical and Pharmaceutical University, Department of Neurosurgery, Resear, 附属病院, 助手 (10161770) ; YAMATANI Kazumasa Toyama medical and Pharmaceutical University, Department of Neurosurgery, Resear, 附属病院, 助手 ; NAKADA Junichi Toyama Medical and Pharmaceutical University, Department of Neurosurgery, Resear, 医学部, 助手 (50143855)
• Project Period (FY): 1987 – 1989
• Keywords: Malignant Glioma / Growth Factor / Tissue Culture / Autocrine / Cell Line

Research Abstract

A cell line was established from an anaplastic astrocytoma from a 69-year-old female and designated as TM-1. The cells expressed S-100 protein, vimentin, and GFAP, characteristic of astrocytes. The tumorigenicity was observed by xenografting them into nude mice, indicating that TM-1 cells were derived from the neoplastic cells of astrocyte origin. Culture medium conditioned by TM-1 cells promoted growth of these cells in serum-free conditions and also of normal rat glial cells and human fibroblasts in serum-depleted culture. The growth promoting effect of conditioned medium was lost by trypsinization and reduced by boiling. By the method of Dot blotting, transforming growth factors (TGF) alpha. and platelet-derived growth factor (PDGF) were found in conditioned medium from TM-1 cells. Colony- forming activity of NRK49 cells cultured in soft-agar medium was promoted by conditioned medium. Furthermore, a large number of TM-1 cells were positive for EGF-receptor by StreptABC method. These findings suggest the following conclusions: 1. The conditioned medium from TM-1 cells contains factors that stimulate proliferation of TM-1 cell itself and possibly also DNA replication. 2. PDGF- and TGFalpha-like growth factors are expressed in TM-1 cells and excreted out of the cells. 3. The observed factors appear to be less stable in heat treatment compared to PDGF and TGFalpha.