1988 Fiscal Year Final Research Report Summary

Immunological Study of Periapical Exudate, Polymorphonuclear Leucocyte Function in Periapical Lesions

Research Project

Project/Area Number	62570865
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Conservative dentistry
Research Institution	Nihon University
Principal Investigator	TSURUMACHI Tamotsu Nihon University, Assistant Professor, 歯学部, 講師 (60139201)
Project Period (FY)	1987 – 1988
Keywords	Periapical lesions / Periapical exudate / Polymorphonuclear leucocyte / 免疫学的検索
Research Abstract	Periapical exudate(PE) are considered to be key components in evaluating the conditions of periapical lesions. Due to their clinical availability, inflammatory cells from PE have been used as a reliable clinical index of disease activity. The aim of the present study was to characterize some functional and morphological aspects of periapical exudate polymorphonuclear leucocytes(PE-PMN) in periabical lesions. The study sample comprised 20 patients referred for endodontic treatment of periapical lesions. In this study, PE-PMN were examined for viability, NBT reduction, FITC conjugated beads phagocytosis and ultrastructure. It is suggested that PE-PMN may exhibit redused bactericidal activity and phagocytic activity compared with peripheral blood polymorphnuclear leucocytes(PB-PMN) from patients, resulting in reduced resistance to infection.