1988 Fiscal Year Final Research Report Summary
Research for Cell Behavior of Osteoclasts Receiving Mechanical Pressure
| Project/Area Number |
62570911
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KURIHARA Saburo Tokyo Medical and Dental University, School of Dentistry, 歯学部, 講師 (70126225)
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| Co-Investigator(Kenkyū-buntansha) |
IIDA Jun-ichiro Tokyo Medical and Dental University, School of Dentistry, 歯学部, 助手 (90151232)
MIURA Fujio Tokyo Medical and Dental University, School of Dentistry, 歯学部, 教授 (90013789)
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| Project Period (FY) |
1987 – 1988
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| Keywords | osteoclast / pressure / direct mechanical stimulus / activation / PMA / cell fusion of osteoclast / 変性型破骨細胞 |
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| Research Abstract |
The research projects in this grant of cell behavior of osteoclasts during mechanical pressure in vitro were divided into four groups: 1)Pressure into closed circuit of osteoclast culture system, 2)Direct mechanical pressure onto osteoclasts, 3)Behavior of activated osteoclasts for bone resorption and 4)Serial cinematograpic observations on degenarated osteoclast.
The results were summarized as follows: 1)The increase of numbers of preosteoclasts as well as multinucleated osteoclasts and the activated bone resorption were observed by means of 16mm cinematography after indirect pressure of about 50mmHg was given into closed circuit of Rose chamber culture system. 2)Direct pressure of culture medium from a jet nozzle of microcapillary to osteoclasts showing bone resorption. These cells showed inhibition of bone resorption just after reception of the direct mechanical pressure. The cells showed the same pattern of the cell behavior after reception of direct mechanical pressure by microcapillary itself. 3)Osteoclastic cell fusion which represents one process of active bone resorption by osteoclasts, were recorded by cinematography. The other process of the activity was demonstrated by using Phorbol Myristate acetate (PMA) onto an osteoclast by direct administration through microcapillary. 4)It was also recorded by cinematography that an osteoclast showed degenerative pattern after long term culture and that it was finally phagocytized by macrophases around the cell.
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