1989 Fiscal Year Final Research Report Summary
Construction of a new centrifuge microscope and its application on biological sciences
| Project/Area Number |
62840021
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理学
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| Research Institution | HITOTSUBASHI UNIVERSITY |
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Principal Investigator |
KAMITSUBO Eiji Hitotsubashi Univ., Dept. Comm., Prof., 商学部, 教授 (60111697)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUYAMA Munehiro The Univ. of the Air, Assoc. Prof., 助教授 (90131010)
YOSHIMOTO Yasuaki Kansai Medical School, Prof., 教養部, 教授 (30124225)
HIRAMOTO Yukio The Univ. of the Air, Prof., 教授 (50011440)
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| Project Period (FY) |
1987 – 1989
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| Keywords | centrifuge microscope / cytoplasmic streaming / echinoderm egg / plasmodial cytoplasmic streaming / characean cell / Vallisneria / 原形質流動 / 非接触電気刺激法 |
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| Research Abstract |
We constructed a new centrifuge microscope of the stroboscopic type(CMS-11). The CMS-11 is composed of a video-enhanced contrast microscope combined with a stroboscopic light source, a mechanism for centrifugation of a specimen, and a control panel for triggering the light source synchlonously with the rotation of the specimen [for detail see Kamitsubo et al Protoplasma (1989) 152:148-155].
Using the new CMS-11 the cyto lasmic streaming in characean internodal cells, was studied (Kamitsubo et al 1989). Plasmodial cytoplasmic streaming under centrifugal acceleration was observed with the CMS-11 (Kamitsubo 1989). The mechanical structure of the cytoplasm of the echinoderm egg was determined by "Gold particle method" using centrifuge microscope (Kaneda et al 1990). Using the CMS-11, effects. of light irradiation on the passive movement of chloroplasts in dark-adapted Vallisneria mesophyll cells were examined (Takagi et al 1989).
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