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1990 Fiscal Year Final Research Report Summary

Molecular Physiology of Axoplasmic Transport

Research Project

Project/Area Number 63304032
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionYokohama City University

Principal Investigator

TAKENAKA Toshifumi  Yokohama, City Univ. Physiology, Prof.,, 医学部, 教授 (00045999)

Co-Investigator(Kenkyū-buntansha) AMANO Junichiro  Kyushu Dental Univ. Physiology, Prof., 歯学部, 教授 (60076015)
CHIHARA Etsuo  Kyoto Univ,. Ophtalmology, Lecture., 医学部, 講師 (20111958)
FUJISAWA Koushiro  Tokyo Metropolian Instit. for Neurosci., Chairman., 神経病理学研究室, 参事研究員 (90073064)
KOMIYA Yoshiaki  Gunma Univ., Biochem., Prof., 医学部, 教授 (50010046)
HIROKAWA Nobutaka  Tokyo Univ., Anatomy, Prof.., 医学部, 教授 (20010085)
Project Period (FY) 1988 – 1990
Keywordsaxoplasmic transport / kinesin / tissue cultured cell / neuronal dystrophy / microtubule / cytoskelet / neurotransmitter / MAPS
Research Abstract

The molecular physiological study of axoplasmic transport has been done in the three fields, cell cytoskeletal, physicobiochemical, and clinical medicine. Kinesin was found on the membrane organella moving anterogradely and dynein was found on the membrane organella moving both anterogradely and retrogradely. Microtubule was divided into three types, depolymerized, dynamic and stable types. These three types changes each other depending on physiological and pathological condition. These experiments were done 35 S-methionin labeling. The axoplasmic transport of mitochondria was studied by the video-enhanced microscope. The number of mitochondria passing though axon was twice in retrograde direction than in anterograde direction. The size of mitochondria moving retrogradely was about half compared with that of mitochondria moving anterogradely. These data implies that mitochondria divided into two in the terminal portion of axon. The latex beads coated with fluorescent dye was introduced into the cell body by the cell fusion method. This latex beads moving in the axon as the same movements of cell organella. The effect of neurotransmitter or the axoplasmic transport was also examined. Acetylcholin stopped the axoplasmic transport reversibly in the superior cervical ganglion. Muscarine receptor related in this effect. Glaucoma axonal dystrophy was also studied pathologically and was found that the anomaly of protein digestive enzyme was the main reason of this dystrophy. The recovery process of axoplasmic transport was also studied after transplantation. Glaucoma was also studied and its pathogenesis was related with the disorder of the axoplasmic transport.

  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] Takenaka,T.: "Axoplasmic transport of mitochondria in cultured dorsal root qanglion cells" Brain Research. 528. 285-290 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hikawa,N: "Introduction of macromolecules into primary cultured neuronal cells by fusion with erythorocite ghosts" Brain Research. 481. 162-164 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horie,H.: "NGF enhances neurite regeneration from nerveーtransected terminals of young adult and aged mouse dorsal root ganglia" Neuroscience Letters. 121. 125-128 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirokawa,N.: "Brain dynein (MAPIC) localizes on both anterogradely and retrogradely transprted membranous organelles" J.Cell Biology. 111. 1027-1037 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okabe,S.: "Turnover of fluorescently labeled tubuline and actin in the axon" Nature. 343. 479-482 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakata,T.: "Conformational change and localization of calpactin 1 complex involved in exocytosis as revealed by quickーfreeze deepーeth technique and immunocytochemistry" J.Cell Biology. 110. 13-25 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisanaga,S.: "Molecular architecture of neurofilament. 1.Subunit arrangement of neurofilamentーL protein in the intermediate sized filament" J.Molecular Biology. 211. 857-869 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisanaga,S.: "Dephosphorylation ーinduced interactions of neurofilaments with microtubules" J.Biological Chemistry. 265. 21852-21858 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Igarashi,M.: "Vinculin is one of the major endogenous substrates for intrinsic tyrosine kinases in neuronal growth cones isolated from fetal rat brain" Europian J.Biochem.193. 551-558 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tashiro,T.: "Stable and dynamic forms of cytoskeletal proteins in slow axonal transport" J.Neuroscience. 9.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koike,H.: "Computer analysis of threeーdimensional structure of neurons using videomicroscopy" Neuroscience Research. sup.11. S87 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita,S.: "Axonal transport of acetylcholineesterase in sciatic nerve of mdx mouse" J.Niurological Science. 92. 267-279 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirokawa,N.: "The cytoskeletal architecture of the presynaptic terminal and molecular structure of synapsin 1" Journal of Cell Biology. 108. 111-126 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okabe,S.: "Immunocytochemical localization of mcrotubuleーassociated proteins 1A and 2 in the rat retina" Brain Research. 483. 335-346 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisanaga,S.: "The effects of dephosphorylation on the structure of the projections of neurofilament" Journal of Neuroscience. 9. 959-966 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisanaga,S.: "The molecular structure of adrenal medulla kinesin" Cell Motility and cytoskeleton. 12. 264-272 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okabe,S.: "Rapid turnover of microtubuleーassociated protein 2 in the axon" Proc.Natl Acad Sci.USA. 86. 4127-4131 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okabe,S.: "Incorporation and turnover of biotinーlabeled actin microinjected into fibroblastic cells : An immunoelectron microscopic study" Journal of Cell Biology. 109. 1581-1595 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Komiya,Y.: "Effects of taxol on slow and fast axonal transport" Cell Motil.Cytoskel.11. 151-156 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zimmermann,H.: "Axonal transport studied in a single vertebrate neuron: the giant electromotor neuron of the electric catlish,Malapーterurus electricus" Neurosci.Res.6. 248-256 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koike,H.: "Silictive axonal transport in a single cholinergic axon of AplysiaーRole of colchicine resistant microtubules" Neuroscience. 32. 539-555 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujisawa,H.: "study of axonal dystrophy.111. Posterior funiculus and posterior column of ageing old rats" Acta Neuropathol.(Berl.). 76. 115-127 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazui,H.: "Radiculoneuropathy of ageing rats: a quantitative study" Neuropathol.Appl.Neurobiol.14. 137-156 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chihara,E.: "Studies on mid brain lesion of patients with down ward gaze paralysis by NMRーCT" Acta Concil Ophthalmol,(Roma). 25. 1741-1744 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chihara,E.: "Atypical nerve fiber defects in myopic eyes with high tension glaucoma" Arch.Ophthalmol.108. 228-232 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koshima,I.: "Experimental study of vascularized muscle : Multifactorial analysis of muscle regeneration follwing denervation" J.Reconstr.Microsurg.5. 225-230 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshino,Y.: "Innervation of the tooth pulp by the mesencephalic trigeminal nucleus in the cat : A retrograde horseradish peroxidase study" Brain Research. 503. 152155 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takenaka,T.: "Metabolic reguration of membrane function" Armenian Acad emy of Sciences, 283 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹中 敏文: "ニュ-ロンとリセプタ-" 倍風館, 163 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹中 敏文、野沢 昭典 訳: "神経細胞の桟能と 軸索輸送" 秀文インタ-ナショナル, 338 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takenaka, T., Kawakami, T., Hikawa, N. & Gotoh, H.: "Axoplasmic transport of mitochondria in cultured dorsal root ganglion cells." Brain Research. 528. 285-290 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hikawa, N., Horie, H., Kawakami, T., Okuda, K. & Takenaka, T.: "Introduction of macromolecules into primary cultured neuronal cells by fusion with erythorocyte ghosts." Brain Research. 481. 162-164 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horie, H., Bando, Y., Tsu, h & Takenaka, T.: "NGF enhances neurite regeneration from nerve-transected terminals of young adult and aged mouse dorsal root ganglia in vitro." Neuroscience Letters. 121. 125-128 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirokawa, N., Yoshida, T., Sato-Yoshitake, R. & Kawasima, T.: "Brain dynein(MAP1C)localizes on both anterogradely and retrogradely transported membranous organelles." Journal of Cell Biology. 111. 1027-1037 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okabe, S. & Hirokawa, N.: "Turnover of fluorescently labeled tubulin and actin in the axon." Nature. 343. 479-482 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakata, T., Sobue, K. & Hirokawa, N.: "Conformational change and localization of calpactin 1 complex involved in exocytosis as revealed by quick-freeze deep-etch technique and immunocytochemistry." J. Cell Biology. 110. 13-25 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisanaga, S., Ikai. A. & Hirokawa, N.: "Molecular architecture of neurofilament. 1. Subunit arrangement of neurofilament-L protein in the intermediate sized filament." J. Molecular Biology. 211. 857-869 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisanaga, S. & Hirokawa, N.: "Dephosphorylation-induced interactions of neurofilaments with microtubules." J. Biological Chemistry. 265. 21852-21858 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Igarashi, M., Sairo, S. & Komiya, Y.: "Vinculin is one of the major endogeneous substrates for intrinsic tyrosine kinases in neuronal growth cones isolated from fetal rat brain." Europian J. Biochem. 193. 551-558 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tasiro, T. & Komiya, Y.: "Stable and dynamic forms of cytoskeletal proteins in slow axonal transport." J. Neuroscience. 9. 760-768 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koike, H., Umitsu, Y., Imanisi, M. & Shibutani, H.: "Computer analysis of three-dimensional structure of neurons using videomicroscopy." Neuroscience Research. Sup. 11. S87 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamashita, S., Takenaka, H., Sugimoto, S., Chihara, E., Sawada, A., Hamada, M. & Matsukura, S.: "Axonal transport of acetylcholinesterase in sciatic nerve of mdx mouse." J. Neurological Science. 92. 267-279 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takenaka, T., Horie, H., Hori, H. & kawakami, T.: "Effect of arachidonic acid and the other longchain fatty acids on the membrane currents in the squid giant axon." J. Membrane Biol. 106. 141-147, (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takenaka, T., Kawakami, T., Hikawa, N. & Bando, Y.: "Neurotransmitter regulation of axoplasmic transport and neuronal growth." Biomed. Res. (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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