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1990 Fiscal Year Final Research Report Summary

Mechanism of Latency and its Control in Herpesvirus Infections

Research Project

Project/Area Number 63304037
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionKyushu University

Principal Investigator

MORI Ryoichi  Kyushu University, Faculty of Medicine, Professor, 医学部, 教授 (50038692)

Co-Investigator(Kenkyū-buntansha) FURUKAWA Toru  Kanazawa Medical University, Faculty of Medicine, Professor, 教授 (00148157)
MINAMISHIMA Youichi  Miyazaki Medical College, Faculty of Medicine, Professor, 教授 (80041284)
TAKAHASHI Michiaki  Osaka University, Research Institute for Microbial Diseases, Professor, 微生物病研究所, 教授 (50029758)
SHIMIZU Yoshinobu  Tohoku University, Faculty of Dentistry, Associate Professor, 歯学部, 助教授 (20005078)
NII Shiro  Okayama University, Faculty of Medicine, Professor, 医学部, 教授 (40029757)
Project Period (FY) 1988 – 1990
KeywordsHerpesvirus / Herpes simplex virus / Varicella-zoster virus / Cytomegalovirus / HHV-6 / Latent infection / Reactivation
Research Abstract

The mechanisms of latent infection and reactivation from latency of herpesviruses, especially herpes simplex virus (HSV), varicella zoster virus(VZV), cytomegalovirus (CMV) and human herpes virus No.6 (HHV-6) were investigated as follows;
Immune defense mechanism against HSV infection was analysed using SCID and nude mice. One of these studies showed that HSV could latently infect in these immunocompromised mice and it was reactivated by pregnancy (Mori).
To distinguish HSV-1 from HSV-2 infection, type-specific DNA regions were searched for and the basic conditions for DNA diagnosis of them were determined (Kurimura).
A comparison between strains of HSV-1 was performed as for the ability of latent infection and reactivation (Nii).
The nervous system as target site of HSV infection and latency was analysed in detail (Kurata).
The mode of latent infection and reactivation of HHV-6 and VZV was analysed in vitro and in vivo. For this purpose, the existence of these viruses in human was examined by PRC. The results showed that HHV-6 DNA was detected both in CD4^+ lymphocytes and in macrophages during the acute phase of exanthem subitum and it was detected only in macrophages during the recovery phase of the disease (Tskahashi).
The target organs and the mode of latent infection of human CMV were investigated by PCR (Furukawa).
The titer of complement-dependent neutralizing antibodies against HCMV was examined during pregnancy in which HCMV become to be excreted from the cervix of the uterus and the influence of their change on reactivation of the virus was discussed (Tanaka).
The significance of CMV infection in Sjogren's syndrome was examined by analysing autoimmune antibodies (Shimizu).
Molecular analysis of lethal and persistent infections was performed by using attenuated mouse CMV (Minamishima).

  • Research Products

    (59 results)

All Other

All Publications (59 results)

  • [Publications] Hidaka,Y.Sakuma,S.Kumano,Y.minagawa,H.Mori,R.: "Characterization of glycoprotein C-negative mutants of herpes simplex virus type 1 isolated from a patient with keratitis" Arch.Virol.113. 195-207 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kumano,Y.Yamoto,M.Inomata,H.Sakuma,S.Hidaka,Y.Minagawa,H.Mori,R.: "Recurrent Herpetic Keratitis:Failure to Detect Herpes simplex Virus Infection Using the Syva MicroTrak^<TM> HSV1/HSV2 Direct Specimen Identification/Typing Test" Ophthalmologica. 201. 169-173 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Li,Yu.-Yu.Hidaka,Y.Kino,Y.Mori,R.: "Seroepidemiology of Herpes Simplex Virus Type 1 in Yanji,Jilin,China" Microbiol.Immunol.34. 551-555 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arao,Y.Yoshida,M.Bai,Z.Kori,Y.Nakatsukasa,A.Takei,Y.Aoji,K.Yamada,M.Uno,F.Miyoshi,K.Nii,S.: "Detection of Varicella-Zoster Virus DNA by Field-Inversion Gel Electrophoresis" Microbiol.Immunol.34. 269-282 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida,M.Uno,F.Yamanishi,.K.Ikuta,K.Kurata,T.Nii,S.: "Electron microscopic study of human herpesvirus-6(HHV-6)" in Electron Microscopy. 3. 384-385 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nii,S.Yoshida,M.Uno,F.Kurata,T.Ikuta,K.Yamanishi,K.: "Replication of Human Herpesvirus 6(HHV-6):Morphological Aspects" in Immunobiology and Prophylaxis of Human Herpesvirus Infections. 19-28 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nii,S.Yoshida,M.Uno,F.Bai Z.Arao,Y.Yamada,M.Kurata,T.Ikuta,K.Yamanishi,K.: "Electron microscopic observations of human herpesvirus-6" in Recent Development of Electron Microscopy.1990. (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arao,Y.Hatano,A.Yamada,M..Uno,F.Nii,S.: "Reactivatable latency of three avirulent strains of herpes simplex virus type 1 after intranasal inoculation in mice" Acta Med,Okayama. 45. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arao,Y.Hatano,A.Yamada,M.Uno,F.Nii,S.: "Neurovirulent strains of herpes simplex virus type 1 are not necessarily competent for reactivatable latency" Acta Med,Okayama. 45. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水 義信: "口腔ヘルペス群ウイルス感染と免疫" 歯科ジャーナル. 31. 990-998 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada,O.Matsumoto,T.Nakashima,M.Hagari,S.Kamahora,T.Ueyama,H.Kishi,Y.Uemura,H.Kurimura,T.: "A new method for extracting DNA or RNA for polymerase chain reaction" J.Virol.Methods. 27. 203-210 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 栗村 敬: "HIV感染におけるHHV-6の関与" 臨床免疫. 22. 446-448 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松本 俊也、釜洞 俊雄、栗村 敬: "単純ヘルペスウイルス1型、2型特異的識別DNA診断法" 臨床とウイルス. 18. 49-52 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurata,T.Iwasaki,T.Sata,T.Wakabayashi,T.Yamaguchi,K.Okuno T.Takei,Y.: "Viral pathology of human herpesvirus 6 infection" in Immunobiology and prophylaxis of human Herpesvirus Infections. 39-47 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishij,K.Kurata,T.Nomura,Y.: "Experiments on herpes simplex viral infections of the facial nerve in the tympanic cavity" Eur.Arch.Otorhinolaryngol.247. 165-167 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito,M.Kamiya,T.Kitamura,K.Sakurai,M.Ihara,T.kamiya,H.Kurata,T.: "Rapid diagnosis of varicella zoster virus(VZV)infection in immunocompromized children by derect immunofluorescence stainning using mouse monoclonal antibodies" Mie Med.J.40. 81-84 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujiwara,Y.Yanagihara,N.Kurata,T.: "Middle ear mucosa in Ramsay Hunt syndrome" Ann Otol,Rhinol & Laryngol. 99. 359-362 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimidbauer,M.Budka,H.Pilz,P.Kurata,T.Hondo,R.: "Presence,distribution,and spread of productive varicella-zoster virus(VZV)infection in nervous tissues" Acta Neuropathol. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okuno,T.Shao,H.Asada,H.Shiraki,K.Takahashi,M.Yamanishi,K.: "Analysis of a glycoprotein of human herpesvirus6(HHV-6)using monoclonal antibodies" Virology. 176. 625-628 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okuno,T.Higashi,K.Shiraki,K.Yamanishi,K.Takahashi,M.Kokado Y.Ishibasi,M.Takahara,S.Sonoda,T.Tanaka,K.Bada,K.Yabuuchi,H.Kurata,T.: "Human herpesvirus 6 infections in renal transplantation" Transplantation. 49. 519-522 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kido,S.Kondo,K.Kondo,T.Morishima T.Takahashi,M.Yamanishi,K.: "Detection of human herpesvirus 6 DNA in throat swabs by polymerase chain reaction" J.Med.Virol.32. 139-142 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondo,K.Hayakawa,Y.Mori,H.Sato,S.Kondo,T.Takahashi,K.Minamishima,Y.Takahashi,M.Yamanishi,K.: "Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA in peripheral blood of patients with exanthem subitum" J.Clin.Microbiol.28. 970-974 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiraki,K.Mukai,T.Okuno T.Yamanishi,K.Takahashi,M.: "Physicochemical charactorization of human herpesvirus 6 infectivity" J.Gen.Vorol.(1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondo,K.Kondo,T.Okuno,T.Takahashi,M.Yamanishi,K.: "Latant infection of human herpesvirus 6(HHV-6)in human monocyte/macrophages" J.Gen.Virol.(1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kido,S.Ozaki,T.Asada,H.Higashi,K.Kondo,K.Hayakawa,Y.Morishima,T.Takahashi,M.Yamanishi,K.: "Detection of varicella-zoster virus(VZV)DNA in clinical samples from patients with VZV by the polymerase chain reaction" J.Clin.Microbiol.29. 76-79 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi,M.Baba,K.Horiuchi,K.Kamiya,H.Asano,Y.: "A live varicella vaccine" in Immunobiology and Prophylaxis of Human herpesvirus Infections. 49-58 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ibusuki,K.Minamishima,Y.: "Effect of Chlorella vulgaris extracts on murine cytomegalovirus infections" Nat.Immun.Cell Growth Regul.9. 121-128 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kumura,K.Ibusuki,K.Minamishima,Y.: "Independent existence of mutations responsible for temperature sensitivity and attenuation in a mutant of murine cytomegalovirus" Virology. 175. 572-574 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Xu-Bin: "β_2 microglobulin Receptor is Located on a Major Human Cytomegalovirus Protein 65 kDa Sharing the Site of IgG Fc Receptor." J.Kanazawa Med.Univ.15. 142-148 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 村山 次哉、古川 宣、地崎 赴美子、館野 恵美子、平井 莞二、: "PCR法のサイトメガロウイルス診断への応用" 臨床とウイルス. 18. 345-348 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 古川 宣: "サイトメガロウイルスワクチン" 日本臨床. 48. 743-748 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lopez,C.Mori,R.Roizman,B.Whitley,R.J.,Edt.: "Immunobiology and Prophylaxis of Human Herpesvirus Infections" Plenum Pless,New York and London, 296 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hidaka, Y. Sakuma, S. Kumano, Y. Minagawa, H. Mori, R.: "Characterization of glycoprotein C-negative mutants of herpes simplex virus type 1 isolated from a patient with keratitis" Arch. Virol. 113. 195-207 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumano, Y. Yamamoto, M. Inomata, H. Sakuma, S. Hidaka, Y. Minagawa, H. Mori, R.: "Recurrent Herpetic Keratitis: Failure to Detect Herpes simplex Virus Infection Using the Syva MicroTraK^<TM> HSV1/HSV2 Direct" Ophthalmologica. 201. 169-173 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li, Yu-Yu. Hidaka, Y. Kino, Y. Mori, R.: "Seroepidemiology of Herpes Simplex Virus Type in Yanji, Jilin, China" Microbiol. Immunol.34. 269-282 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arao, Y. Yoshida, M. Bai, Z. Kori, Y. Nakatsukasa, A. Takei, Y. Aoji, K. Yamada, M. Uno, F. Miyoshi, K. Nii, S.: "Detection of Varicella-Zoster Virus DNA by Field-Inversion Gel Electrophoresis" Microbiol. Immunol.34. 269-282 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, M. Uno, F. Yamanishi, K. Ikuta, K. Kurata, T. Nii, S.: "Electron microscopic study of human herpesvirus-6 (HHV-6)" Electron Microscopy. 3. 384-385 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nii, S. Yoshida, M. Uno, F. Kurata, T. Ikuta, K. Yamanishi, K.: "Replication of Human Herpesvirus 6 (HHV-6): Morphological Aspects" Immunobiology and Prophylaxis of Human Herpesvirus Infections. 19-28 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nii, S. Yoshida, M. Uno, F. Bai, Z. Arao, Y. Yamada, M. Kurata, T. Ikuta, K. Yamanishi, K.: Recent Development of Electron Microscopy 1990. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arao, Y. Hatano, A. Yamada, M. Uno, F. Nii, S.: "Reactivatable latency of three avirulent strains of herpes simplex virus type 1 after intranasal inoculation in mice" Acta Med, Okayama. 45. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arao, Y. Hatano, A. Yamada, M. Uno, F. Nii, S: "Neurovirulent strains of herpes simplex virus type 1 are not nevessarily competent for reactivatable latency" Acta Med, Okayama. 45. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, O. Matsumoto, T. Nakashima, M. Hagari, S. Kamahora, T. Ueyama, H. Kishi, Y. Uemura, H. Kurimura, T.: "A new method for extracting DNA or RNA for polymerase chain reaction" J. Virol. Methods. 27. 203-210 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurata, T. Iwasaki, T. Sata, T. Wakabayashi, T. Yamaguchi, K. Okun, T. Takei, Y.: "Viral pathology of human herpesvirus 6 infection" Immunobiology and prophylaxis of human Herpesvirus Infections. 39-47 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii, K. Kurata, T. Nomura, Y.: "Experiments on herpes simplex viral infections of the facial nerve in the tympanic cavity" Eur. Arch. Otorhinolaryngol. 247. 165-167 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, M. Kimiya, T. Kitamura, K. Sakurai, M. Ihara, T. Kimiya, H. Kurata, T.: "Rapid diagnosis of varicella zoster virus(VZV) infection in immunocompromized children by direct immunofluorescence staining using mouse monoclonal antibodies" Mie Med. J.40. 81-84 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujiwara, Y. Yanagihara, N. Kurata, T.: "Middle ear mucosa in Ramsay Hunt syndrome" Ann Otol, Rhinol & Laryngol. 99. 359-362 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimidbauer, M. Budka, H. Pilz, P. Kurata, T. Honda, R.: "Presence, distribution, and spread of productive varicella-zoster virus (VZV) infection in nervous tissues" Acta Neuropathol. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuno, T. Shao, H. Asada, H. Shiraki, K. Takahashi, M. Yamanishi, K.: "Analysis of a glycoprotein of human herpesvirus 6 (HHV-6) using monoclonal antibodies" Virology. 176. 519-522 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuno, T. Higashi, K. Shiraki, K. Yamanishi, K. Takahash, M. Kokado, Y. Ishibashi, M. Takahara, S. Sonoda, T. Tanaka, K. Baba, K. Yabuuchi, H. Kurata, T.: "Human herpesvirus 6 infections in renal transplantation" Transplantation. 49. 519-522 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kido, S. Kondo, K. Kondo, T. Morishima, T. Takahashi, M. Yamanishi, K.: "Detection of human herpesvirus 6 DNA in throat swabs by polymerase chain reaction" J. Med. Virol.32. 139-142 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondo, K. Hayakawa, Y. Mori, H. Sato, S. Kondo, T. Takahashi, K. Minamishima, Y. Takahashi, M. Yamanishi, K.: "Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA in peripheral blood of patients with exanthem subitum" J.Clin. Microbiol.28. 970-974 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiraki, K. Mukai, T. Okuno, T. Yamanishi, K. Takahashi, M.: "Physicochemical characterization of human herpesvirus 6 infectivity" J.Gen. Virol. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondo, K. Kondo, T. Okuno, T. Takahashi, M. Yamanishi, K.: "Latent infection of human herpesvirus 6 (HHV-6) in human monocyte/macrophages" J. Gen. Virol.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kido, S. Ozaki, T. Asada, H. Higashi, K. Kondo, K. Hayakawa, Y. Morishima, T. Takahashi, M. Yamanishi, K.: "Detection of varicella-zoster virus(VZV) DNA in clinical samples from patients with VZV by the polymerase chain reaction" J. Clin. Microbiol.29. 76-79 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, M. Baba, K. Horiuchi, K. Kamiya, H. Asano, Y.: "A live varicella vaccine" Immunobiology and Prophylaxis of Human herpesvirus Infections. 49-58 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ibusuki, K. Minamishima, Y.: "Effect of Chlorella vulgaris extracts on murine cytomegalovirus infections" Nat. Immun. Cell Growth Rgul.9. 121-128 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kumura, K. Ibusuki, K. Minamishima, Y.: "Independent existence of mutations responsible for temperature sensitivity and attenuation in a mutant of murine cytomegalovirus" Virology. 175. 572-574 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Xu-Bin: "beta_2 microglobulin Receptor is Located on a Major Human Cytomegalovirus Protein 65 kDa Sharing the Site of IgG Fc Receptor" J. Kanazawa Med. Univ.15. 142-148 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lopez, C. Mori, R. Roizman, B. Whitley, R.J., Edt.: "Immunobiology and Prophylaxis of Human Herpesvirus Infections" Immunobiology and Prophylaxis of Human Herpesvirus Infections Plenum Pless, New York and London. 296. (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-03  

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