1989 Fiscal Year Annual Research Report

薬物応答におけるオルガネラ間協調の解析

Research Project

Project/Area Number	63304054
Research Institution	Hiroshima University
Principal Investigator	石橋貞彦広島大学, 医学部, 教授 (90012616)
Co-Investigator(Kenkyū-buntansha)	野村靖幸北海道大学, 薬学部, 教授 (00034041) 市川厚京都大学, 薬学部, 教授 (10025695) 名取俊二東京大学, 薬学部, 教授 (50012662) 加藤敬太郎九州大学, 薬学部, 教授 (70037571) 市原明徳島大学, 酵素科学研究センター, 教授 (40035374)
Keywords	薬物応答 / 代謝制御 / オルガネラ / 細胞膜 / 細胞核 / リソソ-ム / 小胞体 / 細胞質因子
Research Abstract	細胞の刺激への応答は、オルガネラ等各反応部位の素反応について研究が深められているが、実際の反応は細胞質要因を含め種々のオルガネラの協調において行われている。本研究はこの協調という観点に立ち、薬物への応答の解析を総合的に行うことを目的として組織されたものであって、平成元年12月26日には全員で班会議および公開講演会を広島市で行い、成果を総合討論した。 1)細胞膜と細胞核間の協調については、市原、赤松、名取、野村らが中心になって検討を進め、細胞膜受容体に増殖因子、ホルモン、レクチン等のリガンドの結合による情報が細胞核に伝達される過程、核酸合成細胞増殖、酵素誘導等が発現する過程について、多くの新しい知見が得られた。 2)細胞質諸要素と細胞核間の協調については、大場、五十嵐らが主として検討し、ポリアミン産生系等の細胞質酵素やホルモンの細胞内受容体等の細胞質要素の変動とクロマチン機能等との協調関係を解析した。 3)細胞膜とリソソ-ム、小胞体等との協調の解析は、加藤、高野らが主として担当し、細胞膜におけるエンドサイト-シスやエキソサイト-シスとリソソ-ム機能との協調、リソソ-ム酵素の産生等についての新しい事実を明らかにした。 4)細胞膜と細胞質要素との協調に関しては、井上、市川、石橋らを中心に解析を進めた。細胞膜機能が細胞質要素と結合解離により制御される、あるいはその逆の例が近年種々認められているが、細胞膜リン脂質代謝酵素の細胞質への放出、プロスタグランジン受容体とその代謝制御への関与、細胞質因子の細胞膜への移行とその意義などについて多くの新しい知見が得られた。これらの具体的成果の一端は、II.の研究発表に示すようである。

Research Products

(19 results)

All Other

All Publications (19 results)

[Publications] Chiseko Noda: "Requirements of both glucocorticoids and glucagon as co-inducers for activation of transcription of the serine dehydratase gene in cultured rat hepatocytes" J.Biol.Chem.263. 14764-14768 (1988)
[Publications] Kazuhide Hasegawa: "Isolation and characterization of a chinese hamster ovary cell mutant with altered regulation of phosphatidylserine biosynthesis" J.Biol.Chem. 264. 19887-19892 (1989)
[Publications] Osamu Kuga: "Abortive infection with Sindbis of a chinese hamster ovary cell mutant defective in phosphatidylserine and phosphatidyl-ethanolamine biosynthesis" Biochim.Biophys.Acta. 986. 61-69 (1989)
[Publications] Ayako Kobayashi: "Cloning and in vitro transcription of the Sarcophaga lectin gene" Biochim.Biophys.Acta. 1009. 244-250 (1989)
[Publications] Michihisa Tohda: "Effects of antidepressants on serotonin-evoked current in Xenopus oocytes injected with rat brain mRNA" Eur.J.Pharmacol.166. 57-63 (1989)
[Publications] Yoichi Taya: "In vitro phosphorylation of the tumor suppressor gene RB protein by mitosis-specific histone H1 kinase" Biothem.Biophys.Res.Commun.164. 580-596 (1989)
[Publications] Makoto Murakami: "In vitro release and clearance of rat platelet phospholipase A_2" Biochim.Biophys.Acta. 1005. 270-276 (1989)
[Publications] Satomichi Yoshimura: "Prostaglandin D_2 receptor of mastocytoma P-815 cells-possible regulation by phosphorylation and dephosphorylation" Biochim.Biophys.Acta. 981. 67-76 (1989)
[Publications] Kimio Yatsunami: "Effect of tunicamycin on functions of PGE_1 receptors from mouse mastocytoma P-815 cells" Biochim.Biophys.Acta. 1051. 94-99 (1990)
[Publications] Toshiaki Ohtsuka: "Stimulatory effects of a short chain phosphatidate on superoxide anion production in guinea pig polymorphonuclear leukocytes" J.Biochem.106. 259-263 (1989)
[Publications] Masaki Ozawa: "Synergism between protein kinase C activator and fatty acids in stimulating superoxide anion production in guinea pig polymorphonuclear leukocytes" Arch.Biochem.Biophys.273. 491-496 (1989)
[Publications] Toshihiro Ogasawara: "Effect of polyamines on globin synthesis in a rabbit reticulocyte polyamine-free protein synthetic system." J.Biochem.105. 164-167 (1989)
[Publications] Tomohiko Kakegawa: "Effect of polyamine related tetraamines on anti-ulcerogenic activity and anti-H^+,K^+-ATPase activity" Res.Commun.Chem.Path.Pharm.64. 395-406 (1989)
[Publications] Masaru Himeno: "Isolation and sequencing of a cDNA clone encoding 107kDa sialoglycoprotein in rat liver lysosomal membranes" FEBS Lett.244. 351-356 (1989)
[Publications] Koji Furuno: "Biochemical analysis of the movement of a major lysosomal membrane glycoprotein in the endocytic membrane system" J.Biochem.106. 717-722 (1989)
[Publications] Osamu Shimasaki: "Accumulation of oleate-rich cholesteryl esters by acetyl-LDL in macrophages in the presence of an acyl-CoA:cholesterol acyltransferase(ACAT)inhibitor(Sandoz 58-035)" Biochemistry International.20. 389-396 (1990)
[Publications] Hiroshi Mizushima: "Purification of rabbit platelet secretory phospholipase A_2 and its characteristics" J.Biochem.105. 520-525 (1989)
[Publications] 石橋貞彦: "ATPと代謝制御" 東京大学出版会, 108 (1989)
[Publications] Sadahiko Ishibashi: "Physiology and pharmacology of transmembrane signalling" Elsevier Science Publishers B.V., 404 (1989)

1989 Fiscal Year Annual Research Report

薬物応答におけるオルガネラ間協調の解析

Principal Investigator

石橋 貞彦 広島大学, 医学部, 教授 (90012616)

Research Products

[Publications] Chiseko Noda: "Requirements of both glucocorticoids and glucagon as co-inducers for activation of transcription of the serine dehydratase gene in cultured rat hepatocytes" J.Biol.Chem.263. 14764-14768 (1988)

[Publications] Kazuhide Hasegawa: "Isolation and characterization of a chinese hamster ovary cell mutant with altered regulation of phosphatidylserine biosynthesis" J.Biol.Chem. 264. 19887-19892 (1989)

[Publications] Osamu Kuga: "Abortive infection with Sindbis of a chinese hamster ovary cell mutant defective in phosphatidylserine and phosphatidyl-ethanolamine biosynthesis" Biochim.Biophys.Acta. 986. 61-69 (1989)

[Publications] Ayako Kobayashi: "Cloning and in vitro transcription of the Sarcophaga lectin gene" Biochim.Biophys.Acta. 1009. 244-250 (1989)

[Publications] Michihisa Tohda: "Effects of antidepressants on serotonin-evoked current in Xenopus oocytes injected with rat brain mRNA" Eur.J.Pharmacol.166. 57-63 (1989)

[Publications] Yoichi Taya: "In vitro phosphorylation of the tumor suppressor gene RB protein by mitosis-specific histone H1 kinase" Biothem.Biophys.Res.Commun.164. 580-596 (1989)

[Publications] Makoto Murakami: "In vitro release and clearance of rat platelet phospholipase A_2" Biochim.Biophys.Acta. 1005. 270-276 (1989)

[Publications] Satomichi Yoshimura: "Prostaglandin D_2 receptor of mastocytoma P-815 cells-possible regulation by phosphorylation and dephosphorylation" Biochim.Biophys.Acta. 981. 67-76 (1989)

[Publications] Kimio Yatsunami: "Effect of tunicamycin on functions of PGE_1 receptors from mouse mastocytoma P-815 cells" Biochim.Biophys.Acta. 1051. 94-99 (1990)

[Publications] Toshiaki Ohtsuka: "Stimulatory effects of a short chain phosphatidate on superoxide anion production in guinea pig polymorphonuclear leukocytes" J.Biochem.106. 259-263 (1989)

[Publications] Masaki Ozawa: "Synergism between protein kinase C activator and fatty acids in stimulating superoxide anion production in guinea pig polymorphonuclear leukocytes" Arch.Biochem.Biophys.273. 491-496 (1989)

[Publications] Toshihiro Ogasawara: "Effect of polyamines on globin synthesis in a rabbit reticulocyte polyamine-free protein synthetic system." J.Biochem.105. 164-167 (1989)

[Publications] Tomohiko Kakegawa: "Effect of polyamine related tetraamines on anti-ulcerogenic activity and anti-H^+,K^+-ATPase activity" Res.Commun.Chem.Path.Pharm.64. 395-406 (1989)

[Publications] Masaru Himeno: "Isolation and sequencing of a cDNA clone encoding 107kDa sialoglycoprotein in rat liver lysosomal membranes" FEBS Lett.244. 351-356 (1989)

[Publications] Koji Furuno: "Biochemical analysis of the movement of a major lysosomal membrane glycoprotein in the endocytic membrane system" J.Biochem.106. 717-722 (1989)

[Publications] Osamu Shimasaki: "Accumulation of oleate-rich cholesteryl esters by acetyl-LDL in macrophages in the presence of an acyl-CoA:cholesterol acyltransferase(ACAT)inhibitor(Sandoz 58-035)" Biochemistry International.20. 389-396 (1990)

[Publications] Hiroshi Mizushima: "Purification of rabbit platelet secretory phospholipase A_2 and its characteristics" J.Biochem.105. 520-525 (1989)

[Publications] 石橋貞彦: "ATPと代謝制御" 東京大学出版会, 108 (1989)

[Publications] Sadahiko Ishibashi: "Physiology and pharmacology of transmembrane signalling" Elsevier Science Publishers B.V., 404 (1989)

石橋貞彦広島大学, 医学部, 教授 (90012616)