1989 Fiscal Year Annual Research Report

細胞増殖阻害因子の分子的多様性と生理的役割

Research Project

Project/Area Number	63304060
Research Institution	Yokohama City University
Principal Investigator	宮崎香横浜市立大学, 木原生物学研究所, 助教授 (70112068)
Co-Investigator(Kenkyū-buntansha)	西川克三金沢大学, 医学部, 教授 (10029960) 名取俊二東京大学, 薬学部, 教授 (50012662) 田中亮名古屋大学, 歯学部, 教授 (90094383) 鈴木不二男大阪大学, 歯学部, 教授 (40028717) 今西二郎京都府立大学, 医学部, 教授 (40112510)
Keywords	増殖阻害因子 / 増殖因子 / 動物細胞 / 細胞分裂 / 増殖調節 / 癌 / 細胞培養 / 精製
Research Abstract	生体内に存在する多様な細胞増殖阻害因子の生理的役割を明らかにするために、各班員が異なる生体材料に存在する増殖阻害因子については以下の研究を行った。 1)ウサギの血清に存在する腫瘍細胞増殖阻害因子を約3000倍に精製した。この因子は分子量60k-70kの、酸や熱に不安定な蛋白質で、0.5ng/ml以上の濃度で種々のヒト癌細胞の増殖を阻害した。(宮崎)2)ヒト線維芽細胞が産生する腫瘍変性因子TDFとミドリ十字社が見いだした細胞障害因子の異同を検討した結果、両者は異なる因子であることが分かった。(今西)3)抗腫瘍多糖体の投与によってマウス血清中に誘導される腫瘍退縮因子を、牛肺動脈内皮細胞に対する増殖阻害能を指標にしてさらに精製を進めた。(国元)4)軟骨由来抗腫瘍因子(CATF)の産生細胞株として、幼弱マウス成長助軟骨より新しい細胞株MGC/TI.4を樹立した。(鈴木)5)TGF-β1がTNFやIGN-αと協調的に単球性白血病細胞の増殖を阻害し、マクロファ-ジへの分化を誘導することを見いだした。(武田)6)ラットおよびヒトのグリア細胞が産生する神経芽細胞増殖抑制因子(前者の分子量は71k、後者は10k)をほぼ完全に精製した。(田中)7)センチニクバエの蛹の体液細胞による幼虫細胞破壊作用に、体液細胞の膜表面に存在する分子量200kの蛋白質が関与することを見いだした。(名取)8)新生期マウス由来の増殖阻害因子NBCF(分子量62k)の14個のアミノ酸配列を決定した結果、この因子が既知の蛋白質ではないことが判明した。(三羽)9)線維芽細胞増殖因子bFGFに対する中和モノクロ-ナル抗体が、ヌ-ドマウス体内でヒト扁平上皮癌細胞A431やラット線維肉腫細胞の増殖を強く阻害することを見いだした。(西川)

Research Products
(20 results)

All Other

All Publications (20 results)

[Publications] K.Miyazaki: "Growth inhibitors-molecular diversity and roles in cell proliferation." In Vitro Cell.Develop.Biol.25. 866-872 (1989)
[Publications] K.Miyazaki: "Characterization of fibronectin-hydrolyzing metallo-proteinase secreted from virally transformed rat liver cell line RSV-BRL." Intracellular Protein Catabolism Japan Scientific Societies Press,Tokyo. 559-565 (1989)
[Publications] K.Miyazaki: "Potent growth inhibition of human tumor cells in cuilture by arginine deiminase purified from culture medium of mycoplasma-infected cell line." Cancer Res.(1990)
[Publications] 宮崎香: "ウサギの血清中に存在する腫瘍細胞増殖阻害因子" Human Cell. 3. (1990)
[Publications] K.Okumura-Noji: "Inhibition by dipheny lhydation of growth and morphological differentiation of glial cells;implication of calcium." Neurochem.Int.14. 353-359 (1989)
[Publications] T.Ohsawa: "Expression of tumor necrosis factor at a specific developmental stage of mouse embryo." Devel.Biol.135. 459-461 (1989)
[Publications] 今西芳二郎: "ヒト腫瘍変性因子(TDF)" 松仁会誌. 28. 1-7 (1989)
[Publications] M.Takigawa: "Establishment from mouse growth cartilage of clonal cell lines with responsiveness to parathyroid hormone,alkaline phosphatase activity,and an ability to produce an endothelial cell growth inhibitor." Calcif.Tissue.Int.45. 305-313 (1989)
[Publications] M.Takigawa: "Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes." Cancer Res.49. 3996-4002 (1989)
[Publications] R.Kamijo: "Effects of combinations of transforming growth factor-β1 and TNF on induction of differentiation of human myelogenous leukemic cell lines." J.Immunol.
[Publications] R.Kamijo: "Suppression of TNF-stimulated proliferation of diploid fibroblasts and TNF-induced cytotoxicity against transformed fibroblasts by TGF-β1." Biochem.Biophys.Res.Commun.15. 98-101 (1989)
[Publications] J.Ito: "Induction of astroglial growth inhibition and differentiation by sialosyl cholesterol." Brain Res.4818. 335-343 (1989)
[Publications] S.Kurata: "Dissociation of Sarcophaga peregrina(flesh fly) fat body by pupal hemocytes in vitro" J.Insect.Physiol.35. 559-565 (1989)
[Publications] H.Tanaka: "Identification of a family of insulin-like growth factor II secreted by cultured rat epithelial-like cell line 18,54-SF." Endocrinology. 124. 870-877 (1989)
[Publications] K.Matszaki: "Monoclonal antibodies against heparin-binding growth factor II/basic fibroblast growth factor that block its biological activity:invalidity of the antibodies for tumor angiogenesis." Proc.Natl.Acad.Sci.USA. 86. 9911-9915 (1989)
[Publications] N.Miwa: "Carcinostatic substances secreted from that animal brain." Reports Res.Meat Products. 7. 483-490 (1989)
[Publications] N.Miwa: "Tumor growth-inhibitory glycoprotein secreted from mouse brain at the neonatal stage of the ontogenesis:molecular homogeneity and requirement of the protein conformation for exhibition of the cytostatic action." Biochim.Biophys.Acta.
[Publications] K.Hanai: "Central action of acidic fibroblast growth factor in feeding regulation." Am.J.Physiol.256. 217-223 (1989)
[Publications] 宮崎香: "上皮細胞「無血清細胞マニュアル」" 講談社, 248( P89-104) (1989)
[Publications] 今西二郎: "新しいサイトカイン" 金芳堂, 171 (1990)

1989 Fiscal Year Annual Research Report

細胞増殖阻害因子の分子的多様性と生理的役割

Principal Investigator

宮崎 香 横浜市立大学, 木原生物学研究所, 助教授 (70112068)

Research Products

[Publications] K.Miyazaki: "Growth inhibitors-molecular diversity and roles in cell proliferation." In Vitro Cell.Develop.Biol.25. 866-872 (1989)

[Publications] K.Miyazaki: "Characterization of fibronectin-hydrolyzing metallo-proteinase secreted from virally transformed rat liver cell line RSV-BRL." Intracellular Protein Catabolism Japan Scientific Societies Press,Tokyo. 559-565 (1989)

[Publications] K.Miyazaki: "Potent growth inhibition of human tumor cells in cuilture by arginine deiminase purified from culture medium of mycoplasma-infected cell line." Cancer Res.(1990)

[Publications] 宮崎香: "ウサギの血清中に存在する腫瘍細胞増殖阻害因子" Human Cell. 3. (1990)

[Publications] K.Okumura-Noji: "Inhibition by dipheny lhydation of growth and morphological differentiation of glial cells;implication of calcium." Neurochem.Int.14. 353-359 (1989)

[Publications] T.Ohsawa: "Expression of tumor necrosis factor at a specific developmental stage of mouse embryo." Devel.Biol.135. 459-461 (1989)

[Publications] 今西芳二郎: "ヒト腫瘍変性因子(TDF)" 松仁会誌. 28. 1-7 (1989)

[Publications] M.Takigawa: "Establishment from mouse growth cartilage of clonal cell lines with responsiveness to parathyroid hormone,alkaline phosphatase activity,and an ability to produce an endothelial cell growth inhibitor." Calcif.Tissue.Int.45. 305-313 (1989)

[Publications] M.Takigawa: "Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes." Cancer Res.49. 3996-4002 (1989)

[Publications] R.Kamijo: "Effects of combinations of transforming growth factor-β1 and TNF on induction of differentiation of human myelogenous leukemic cell lines." J.Immunol.

[Publications] R.Kamijo: "Suppression of TNF-stimulated proliferation of diploid fibroblasts and TNF-induced cytotoxicity against transformed fibroblasts by TGF-β1." Biochem.Biophys.Res.Commun.15. 98-101 (1989)

[Publications] J.Ito: "Induction of astroglial growth inhibition and differentiation by sialosyl cholesterol." Brain Res.4818. 335-343 (1989)

[Publications] S.Kurata: "Dissociation of Sarcophaga peregrina(flesh fly) fat body by pupal hemocytes in vitro" J.Insect.Physiol.35. 559-565 (1989)

[Publications] H.Tanaka: "Identification of a family of insulin-like growth factor II secreted by cultured rat epithelial-like cell line 18,54-SF." Endocrinology. 124. 870-877 (1989)

[Publications] K.Matszaki: "Monoclonal antibodies against heparin-binding growth factor II/basic fibroblast growth factor that block its biological activity:invalidity of the antibodies for tumor angiogenesis." Proc.Natl.Acad.Sci.USA. 86. 9911-9915 (1989)

[Publications] N.Miwa: "Carcinostatic substances secreted from that animal brain." Reports Res.Meat Products. 7. 483-490 (1989)

[Publications] N.Miwa: "Tumor growth-inhibitory glycoprotein secreted from mouse brain at the neonatal stage of the ontogenesis:molecular homogeneity and requirement of the protein conformation for exhibition of the cytostatic action." Biochim.Biophys.Acta.

[Publications] K.Hanai: "Central action of acidic fibroblast growth factor in feeding regulation." Am.J.Physiol.256. 217-223 (1989)

[Publications] 宮崎香: "上皮細胞「無血清細胞マニュアル」" 講談社, 248( P89-104) (1989)

[Publications] 今西二郎: "新しいサイトカイン" 金芳堂, 171 (1990)

宮崎香横浜市立大学, 木原生物学研究所, 助教授 (70112068)