1989 Fiscal Year Final Research Report Summary
Biological control of fish viral diseases by the use of bacteria which produce anti viral substances.
| Project/Area Number |
63470120
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Faculty of Fisheries, Hokkaido University |
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Principal Investigator |
KIMURA Takahisa Faculty of Fisheries Professor, 水産学部, 教授 (60001583)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMIZU Mamoru Faculty of Fisheries Assistant, 水産学部, 助手 (40122915)
TAJIMA Kenichi Faculty of Fisheries Associate Professor, 水産学部, 助手 (80002252)
EZURA Yoshio Faculty of Fisheries Associate Professor, 水産学部, 助教授 (80001618)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Fish disease / Auti viral substance / Bioreactor / Biological control / IHNV / OMV / Intestinal microflora |
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| Research Abstract |
The purpose of this study is to control fish viral disease by the use of bacteria which produce antiviral substances and are inherent in the aquatic environment.
Distribution of bacteria containing antiviral substance and its mechanism was studied.
Likewise, application of this bacteria to control fish viral disease was conducted.
Results were summarized as follows:
1. Antiviral-producing bacteria is widely distributed in both freshwater and seawater environments. Most bacteria were identified belonging to Pseudomonas spp.
2. A representative bactdrial strain capable of producing anti-IHNV substance was identified as Pseudomonas fluorescens biovar 1. Antiviral substance produced from this bacteria has a molecular weight of 1,126 and a newly discovered peptide.
3. This substance produced by Pseudomonas fluorescens show antiviral activity against OMV and did not show toxicity to rainbow trout and mice.
4. Antiviral mechanism showed its ability to block receptor of the virus, IHNV.
5. The bacteria, Pseudomonas fluorescens, was immobilized in alginate beads and produced anitiviral substance continuously.
6. Several antiviral-producing bacteria was isolated from fish intestines. A representative bacteria was able to produce antiviral substance in the pellet extract and fish intestine. Artificial infection was done by feeding fish with pellet y inoculated with antiviral-producing bacteria. Results obtained showed that mortality rates were different between fish fed with pellets inoculated with bacteria and fish fed with uninoculated pellets. This data suggests the possibility of this bacteria in controlling fish viral diseases.
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