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1989 Fiscal Year Final Research Report Summary

Biological control of fish viral diseases by the use of bacteria which produce anti viral substances.

Research Project

Project/Area Number 63470120
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionFaculty of Fisheries, Hokkaido University

Principal Investigator

KIMURA Takahisa  Faculty of Fisheries Professor, 水産学部, 教授 (60001583)

Co-Investigator(Kenkyū-buntansha) YOSHIMIZU Mamoru  Faculty of Fisheries Assistant, 水産学部, 助手 (40122915)
TAJIMA Kenichi  Faculty of Fisheries Associate Professor, 水産学部, 助手 (80002252)
EZURA Yoshio  Faculty of Fisheries Associate Professor, 水産学部, 助教授 (80001618)
Project Period (FY) 1988 – 1989
KeywordsFish disease / Auti viral substance / Bioreactor / Biological control / IHNV / OMV / Intestinal microflora
Research Abstract

The purpose of this study is to control fish viral disease by the use of bacteria which produce antiviral substances and are inherent in the aquatic environment.
Distribution of bacteria containing antiviral substance and its mechanism was studied.
Likewise, application of this bacteria to control fish viral disease was conducted.
Results were summarized as follows:
1. Antiviral-producing bacteria is widely distributed in both freshwater and seawater environments. Most bacteria were identified belonging to Pseudomonas spp.
2. A representative bactdrial strain capable of producing anti-IHNV substance was identified as Pseudomonas fluorescens biovar 1. Antiviral substance produced from this bacteria has a molecular weight of 1,126 and a newly discovered peptide.
3. This substance produced by Pseudomonas fluorescens show antiviral activity against OMV and did not show toxicity to rainbow trout and mice.
4. Antiviral mechanism showed its ability to block receptor of the virus, IHNV.
5. The bacteria, Pseudomonas fluorescens, was immobilized in alginate beads and produced anitiviral substance continuously.
6. Several antiviral-producing bacteria was isolated from fish intestines. A representative bacteria was able to produce antiviral substance in the pellet extract and fish intestine. Artificial infection was done by feeding fish with pellet y inoculated with antiviral-producing bacteria. Results obtained showed that mortality rates were different between fish fed with pellets inoculated with bacteria and fish fed with uninoculated pellets. This data suggests the possibility of this bacteria in controlling fish viral diseases.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Kimura,T.,M.Yoshimizu,Y.Kamei,and Y.Ezura: "An antiviral agent against fish viruses produced by Pseudomonas sp.46NW-04A from the aquatic environment." J.Aquatic Animal Health. 1. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura,T.,M.Yoshimizu,Y.Ezura and Y.Kamei: "Recent Advances in Microbial Ecology:Interaction between fish pathogenic viruses and microorganisms in environmental water of fish" Japan Scientific Societies, 587-591 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimizu,M.,H.Myouga,Y.Kamei,Y.Ezura and T.Kimura: "The Proceedings of First International Marine Biotechnology Conference:Production of antiviral substances by bacteria cells immobilized alginate beads." Japan Scientific Societies, (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, T.: "An antiviral agent against fish viruses produced by Pseudomonas sp. 46NW-04A from the aquatic environment." J. Aquatic Animal Health, 1, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, T.: "Interaction between fish pathogenic viruses and microorganisms in environmental water of fish." Japan Scientific Societies Press, 587-591, 1989, Recent Advances in Microbial Ecology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimizu, M.: "Production of antiviral substances by bacterial cells immobilized alginate beads." The Proceedings of First International Marine Biotechnology Conference, 1990.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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