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1990 Fiscal Year Final Research Report Summary

The Studied of Axoplasmic Transport Using Cultured Neuronal Cells by Fusion with Erythrocyte Ghosts.

Research Project

Project/Area Number 63480099
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionYokohama City University

Principal Investigator

TAKENAKA Toshifumi  Yokohama, City Univ., Physiology, Prof.,, 医学部, 教授 (00045999)

Co-Investigator(Kenkyū-buntansha) KAWAKAMI Tadashi  Yokohama, City Univ., Physiology, Lecture., 医学部, 助手 (60177649)
HORI Hideaki  Yokohama, City Univ., Physiology, Lecture., 医学部, 助手 (30117759)
Project Period (FY) 1988 – 1990
Keywordsaxoplamic transport / cell fusion / erythocyte ghosts / cultured cell / dorsal root ganlion / supper cervical ganglion / latex beads / axonal flow
Research Abstract

In order to study the axoplasmic transport, the macromolecules were injected into primary cultured neuronal cells by fusion with human erythrocyte ghosts containing macromolecules. FITC-BSA or FITC-IgG trapped into cultured mouse dorsal root ganglion (DRG) cells using photohaemaagglutinin E (PHA-E) and polyethylene glycol 1000. The injection efficiency was about 30% and 80% of the cells extended neurites like neuronal cells. The latex beads transported in neurites both anterogradely and retrogradely The velocities of beads were 0.53 mu m/sec in anterograde direction and 0.43 mu m/sec in retrograde direction respectively. The velocity depends on the size of beads. The velocity of small beads is faster than that of bigger beads.
Cells were dissociated from the superior cervical ganglion (SCG) of mice and cultured on coverslips for 3 days. The particle movements in neurites and neuronal architecture were observed by video-enhanced microscopy. The movements of particles in neurites becomes slow and finally stops with the application of Acetylcholine. These changes were completely reversible by removing Acetylcholine. Arecoline stopped the axoplasmic transport like acetylcholine. QNX restrained the acetylcholine effects. These pharmacological data show that the effect of acetylcholine related with muscarinic receptor. Inslet Activating Protein (IAP) also restrained the effect of acetylcholine on the axoplasmic transport. When axoplasmic transport was stopped, the transported material dropped off from the microtubule in the neurites. Then from that area the new branches of the neurites grow. This result demonstrates that neurotransmitter can play a prominent role in regulating neuronal architecture as a type of plasticity besides their classical role in neurotransmission.

  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Takenaka,T.: "Axoplasmic transport of mitochondria in cultured dorsal root ganglion cells" Brain Research. 528. 285-290 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horie,H.: "NGF enhances neurite regeneration from nerveーtransected terminals of young adult and aged mouse dorsal root ganglia in vitro" Neuroscience Letters. 121. 125-128 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takenaka,T.: "Neurotransmitter regulation of axoplasmic transport and neuronal growth" Biomedical Research. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horie,H.: "Membrane elasticity of mouse dorsal root ganglion neurons decreases with aging" FBS Letters. 269. 23-25 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hikawa,N.: "Introduction of macromolecules into primary cultured neuronal cells by fusion with erythorocyte ghosts" Brain Research. 481. 162-164 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horie,H.: "Adaptation of cultured mammalian neurons to a hypotonic environment with ageーrelated response." Brain Research. 477. 233-240 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹中 敏文: "ニュ-ロンとリセプタ-" 倍風館, 163 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takenaka,T.: "Metabolic regulation of membrane function" Armenian Academy of Sciences Publishers, 283 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takenaka, T., Kawakani, T., Hikawa, N. & Gotoh, H: "Axoplasmic transport of mitochondria in cultured dorsal root ganglion cells." Brain Research. 528. 285-290 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takenaka, T., Kawakami, T., Hikawa, N. & Bando, Y.: "Neurotransmitter regulation of axoplasmic transport and neuronal growth." Biomed. Res. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horie, H., Bando, Y., Tsu, H. & Takenaka, T.: "NGF enhances neurite regeneration from nerve-transected terminals of young adult and aged mouse dorsal root ganglia in vitro." Neuroscience Letters. 121. 125-128 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takenaka, T., Suleymanian, M. A. & Ayrapetyan, S. N.: "The effects of short-chain fatty acids on membrane electrical properties of snail neurons" Metabolic regulation of membrane function, ed. Ayrapetyan, S. N. Armenian Academy of Sciences Publishers Yerevan. 154-166 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horie, H., Ikuta, S. & Takenaka, T.: "Membrane elasticity of mouse dorsal root ganglion neurons decreases with aging." FEBS Letters. 269. 23-25 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hikawa, N., Horie, H., Kawakami, T., Okuda, K. & Takenaka, T.: "Introduction of macromolecules into primary cultured neuronal cells by fusion with erythrocyte ghosts." Brain Research. 481. 162-164 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hikawa, N., Kawakami, T., Kanou, M. & Takenaka, T.: "Axoplasmic transport of beads injected by fusion with erythrocyte ghosts." Jan. J. Physiol. 39, (Suppl). S. 27 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horie, H., Ikuta, S., Takenaka, T. & Ito, S.: "Adaptation of cultured mammalian neurons to a hypotonic environment with age-related response." Brain Research. 477. 233-240 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horie, H., Kim, S. U. & Takenaka, T.: "Immunofluorescence demonstration of neurofilament polypeptide expression in fetal human neurons in culture." Neurosci. Res. 6. 463-469 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi, M., Yamada, S., Daimon, N., Yamamoto, I., Kawakami, T. & Takenaka, T.: "Electromagnetic mechanism of magnetic nerve stimulation." J. Appl. Phys.66. 1459-1465 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takenaka, T., Horie, H., Hori, H. & Kawakami, T.: "Effect of arachidonic acid and the other longchain fatty acids on the membrane currents in the squid giant axon." J. Membrane Biol.106. 141-147 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawakami, T., Hikawa, N., Kanou, M., Gotoh, H., Takenaka, T. & Horie, H.: "Axoplasmic transport of mitochondria in tissue cultured neuron." Jpn. J. Physiol. 39(Suppl). S45 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gotoh, H., Kawakami, T., Hikawa, N., Kusakabe, S. & Takenaka, T.: "Axoplasmic transport and intracellular calcium during capsaicin action in dorsal root ganglion cells in culuture." Jap. J. Physiol.39, (Suppl). S44 (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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