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1989 Fiscal Year Final Research Report Summary

Use for research materials in Pathology--application for molecular pathology

Research Project

Project/Area Number 63480143
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionKobe University School of Medicine

Principal Investigator

MAEDA Sakan  Kobe University Pathology Professor, 医学部, 教授 (50030911)

Co-Investigator(Kenkyū-buntansha) HORIO Mitsuzou  Kobe Univ. Pathology Assistant, 医学部, 助手 (90209289)
FUJIMORI Takahiro  Kobe Univ. Pathology Assist. Prof., 医学部, 講師 (30095385)
Project Period (FY) 1988 – 1989
KeywordsFormalin fixation / Molecular pathology / Oncogenes / DNA degradation / Gene expression / Culture / Monoclonal antibody
Research Abstract

Department of Pathology has many chance to get materials from surgical operation and autopsy. However, their uses may be limited to histological diagnosis and other trials to use for another purpose must be necessary. Present experiment was undertaken to examine how to use l.fresh, 2.unfixed, and 3.fixed pathological materials for pathological research. In 1., the culture supernatant of human placenta contained the growth factor for human squamous cell lines and it further was examined which factor might be essential for that stimulation. For lung small cell carcinomas, L-cysteine, transferrin, and bathocuproine disulfo hate had also growth activity and L-cysteine was most important. In 2., extraction method for RNA was examined and modification of Rupp and Locker's method might be effective for massive extraction from degenerated tissues. In 3., the mechanism of degeneration of formalin-fixed tissues was examined basically. The extracted and purified DNA was not damaged when it was fixed in 10% formalin.
Because pulsed field gel electrophoresis and histone-DNA recombination experiment revealed the same damage to formalin-fixed tissues when it was extracted by usual DNA extraction method, the mechanical damage was not so important as the mechanism of degradation. Fixation in low temperature and addition of EDTA inhibited the degradation of DNA. Enzymatic activity such as DNase during formalin fixation might have responsibility for DNA degradation. Monoclonal antibodies which were specific to germ cells and germ cell tumors, were generated from formalin-fixed and parrafin-embedded tissues as mouse immunogen.
Several trials might become possible to use many pathological specimens for pathological research. However further experiments may be necessary for multi- purpose use of them.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] M.Yabe-Nagasaka,S.Maeda,O.Mabuchi,O.Misu,S.Nakamura,T.Sugiyama,: "Heterogeneous origin of established Non-T,Non-B cell lines from two adolesce nts with acute lymphoblastic leukemia," Japanese.Journal of Cancer Research. 79. 59-68 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Ishikawa,S.Maeda.,Kamidono,and T.Sugiyama,: "Restriction fragment length polymorphism and activation of c-Ha-ras gene in urothelial cancer." Anticancer Research.8. 915-924 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Takenaka,S.Kitazawa,S.Maeda,T.Kamidono,: "Monoclonal antibodies to human germ cell tumors from“Routine"paraffinembe dded pathological specimens." Histochemistry,(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 武中篤,北沢荘平,小山隆司,平山大介,藤盛孝博,後藤章暢,郷司和男,前田盛,杉山武敏,守殿貞夫: "デスモゾ-ム関連34KD蛋白を認識するモノクロ-ナル抗体の作製と膀胱移行上皮癌の悪性度に関する免疫組織学的検討" 日本泌尿器学会雑誌. 80. 1769-1775 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Yabe-Nagasaka, S.Maeda, O.Mabuchi, O.Misu, S.Nakamura, T.Sugiyama: "Heterogeneous origin of established Non-T,Non-B cell lines from two adolescents with acute lymphoblastic leukemia." Japanese Journal of Cancer Research, 79,59-68,1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.You, S.Maeda, S.Murao, R.Takahashi: "Establishment and characterization of mouse leukemia cell lines L615K and L7212K derived from transplantable murine leukemia maintained in China." Japanese Journal of Cancer Research, 80,444-451,1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kitazawa, S.Maeda, T.Sugiyama: "Immunocytochemical evaluation of abl-gene products in leukemic cell lines." Medical Oncology & Tumor Pharmacotherapy (1990).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Takenaka, S.Kitazawa, S.Maeda, T.Kamidono: "Monoclonal antibodies to human germ cell tumors from "Routine" paraffin-embedded pathological specimens." Histochemistry, (1990).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Tokuda, T.: "Fundamental study on the mechanism of DNA degradation in formaldehyde fixed tissues and its application for diagnoses. (in Japanese)" Kobe Medical Journal in Japanese (51-1).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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