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1990 Fiscal Year Final Research Report Summary

Studies on Mechanism of Proliferation of Lymphocytis to Produce Platelet Associated Immunoglobulin.

Research Project

Project/Area Number 63480240
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionTokyo Jikei University School of Medicine Department of Pediatrics

Principal Investigator

AKATSUKA Jun-ichi  Professor, Department of Pediatrics, Tokyo Jikei Univ. School of Medicine, 医学部・小児科学教室, 教授 (20056550)

Co-Investigator(Kenkyū-buntansha) IKEGAMI Mayumi  Research Assistant, Department of Pediarrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (40184410)
KOBAYASHI Naoaki  Research Assistant, Department of Pediatrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (50186756)
UCHIYAMA Hiroshi  Research Assistant, Department of Pediatrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (10168718)
ISHIDOYA Naoko  Research Assistant, Department of Pediatrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (80168244)
FUJISAWA Koji  Research Assistant, Department of Pediatrics, Tokyo Jikei Univ. School of Medici, 小児科学教室, 助手 (10130197)
Project Period (FY) 1988 – 1990
KeywordsIDIOPATHIC THROMBOCYTOPENIC PURPURA / PAIGG / ELIZA-PLAQUE ASSAY / LAIGG / ANTIGEN COMBINE WITH AUTOIMMUNE ANTIBODY / GPIIb / IIIa
Research Abstract

1) Mechanism of proliferation of PAIgG-secreting lymphocytes.
We developed the new modified methods of ELIZA-plaque assay which could identify and count the lymphocytes to secrete platelet associated IgG (PAIgG) in mouse spleen cells sensitized with human platelets. The proliferation of PAIgG secreting spleen cells of mouse were suppressed with such drugs as cyclophosphamide, bolus prednisolone and prednisolone of long period. However, we could not detect PAIgG-secreting lymphocytes in spleen cells of children with chronic ITP and the peripheral lymphocytes of the patient sensitized with isologous platelets transfusion.
On the other hand, we immunized mouse with IgG fraction of the children whose sera contained platelet bound IgG and produced the polyclonal antibody for antihuman IgG. Using this antibody, we could found incresed number of the lymphocytes combined with IgG (LAIgG) in the peripheral blood of children with chronic ITP, Evans syndrome and ulcerative colitis. Then, the lymphocytes with LAIgG were shown to be T-cells. The student are going to make clear the significance of the increase of lymphocytes with LAIgG in those children.
2) Detection of antigen site on the palatelet membrane combined with autoantibody.
We measured the amount of monoclonal antibodies to conbine with platelet membrane as for platelets of children with chronic ITP, Evans syndrome and thromboasthenia. Five of 22 children with chronic ITP showed decreased amount of monoclonal antibodies combined with platelet membrane. This phenomenon suggested that there were the antigen site to combine with autoimmune platelet antibodies near the epitope of GPIIb/IIIa.

  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] 赤塚 順一 他: "小児ITP患児の発育障害および残存異常、" 厚生省特定疾患、特発性造血障害調査研究班、昭和62年度業績報告、. 248-249 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 赤塚 順一 他: "小児ITP患児の血小板結合免疫グロブリン及び補体の検討" 厚生省特定疾患、特発性造血障害調査研究班、昭和62年度業績報告、. 250-251 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] 内田 浩志 他: "ELIAAーplaque assayによる血小板抗体産生脾細胞の検出、" 医学のあゆみ. 144. 823-824 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] 藤沢 康司 他: "小児急性及び慢性ITPに対するメチルプレドニソロン療法と免疫グロブリン大量療法の比較検討、" 日児誌、. 92. 91-97 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 赤塚 順一: "PAIgGの測定とその臨床的意義、" 日小血会誌、. 2. 1-8 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] 赤塚 順一: ":血小板と免疫、Currents in Hematoimmunology," Excerpta Medica,. 4. 3-8 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] 赤塚 順一: "特発性血小板減少性紫斑病(小児)" ,Medicina,. 25. 2330-2331 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] 藤沢 康司: "血小板機能異常症" 小児内科. 20. 995-1000 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akatsuka J,et al.: "Longーterm followーup studies of children of children with chronic ITP." Blut. 59. 105-108 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安永 幸二郎 他: "完全分子型液状免疫グロブリン(Cー425)大量投与による特発性血小板減少性紫斑病の治療成績、" 基礎と臨床. 23. 3269-3279 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uciyama H.et al.: "ELIZAーplaque assay for detection of platelet antibody secreting cells.I.Studies on optimization of reagent concentrations and culture conditions for enumeration of spotーforming cells." J of Immunological Methods,. 117. 199-203 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 赤塚 順一: ":種々の疾患における脾摘の適応とその遠隔成績" 小児外科. 21. 131-135 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchiyama H,et al.: "ELIZAーplaque assay for the detection of platelet antibodyーsecreting cellsーEffects of various immunosuppressive treatment on platelet antibodysecreting cells." Jikeikai Med J.37. 407-413 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤沢 康司 他: "全血flow cytometryによる慢性ITPにおける自己抗体対応抗原の検討、" 臨床血液、. 31. 1769-1773 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 赤塚 順一: "免疫グロブリン療法の最近の知見、特発性血小板減少性紫斑病、" 小児医学、. 23. 673-691 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池上 真由美、藤沢 康司、赤塚 順一: "小児特発性血小板減少性紫斑病における血小板結合免疫グロブリンに関する研究第1編、フロ-サイトメトリ-による血小板結合グロブリンの検出と血小板形態解折、" 日児誌、. 92. 1866-1873 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池上 真由美、藤沢 康司、赤塚 順一: "小児特発性血小板減少性紫斑病における血小板結合免疫グロブリンに関する研究、第2編、血清中の血小板結合性IgGの同種血小板への作用について、" 日児誌、. 94. 2135-2159 (1990)

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      「研究成果報告書概要(和文)」より
  • [Publications] 赤塚 順一: ":小児の血小板と免疫、血小板1988ー血小板と免疫ー 蔵本 淳、青木 信雄、大熊 稔、日高 弘義、 山崎 博男編" 、科学評論社, 117-129, (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 赤塚 順一: ":小児特発性血小板減少性紫斑病の特徴と治療、小児科Mook,馬場 一雄、小林 登、山田 兼雄、" 金原出版, 119-128 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 赤塚 順一: ":血液疾患に対する免疫グロブリン療法、新小児医学体系、小児医学の進歩、" 153-167 (1989)

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      「研究成果報告書概要(和文)」より
  • [Publications] Fujisawa K, Akatsuka J. et al.: "Comparison of bolus methylprednisolone therapy and high-dose immunoglobulin intravenous infusion of children with acute and chronic ITP" J of Jap Pediatr Soc. 92. 91-97 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akatsuka J.: "Clinical significance of measurement of platelet associated IgG." Jap J of Pediatr Hematol. 2. 1-8 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akatsuka J.: "Platelet and immunology" Excerp Medica. 4. 3-8 (1988)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Akatsuka J.: "Characteristics treatment of children with idiopathic thrombo-cytopenic purpura" Pediatric book. 54. 119-128 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujisawa K, Akatsuka J.: "Thrombocytopathy" Jap J of Pediatr Med. 20. 995-1000 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikegami M, Fujisawa K, Akatsuka J.: "Platelet associated immunoglobulin in idiopathic thrombocytopenic purpura in childhood. Part. 1. Analysis of platelet associated immunoglobulins and platelet volume by flow cytometry." J of Jap Pediatr Soc. 92. 1866-1873 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akatsuka J, Fujisawa K, et al.: "Long-term follow-up studies of children with chronic idiopathic thrombocytopenic purpura" Blut. 59. 105-108 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchiyama H, Akatsuka J.: "ELIZA-plaque assay for the detection of platelet antibody-secreting cells. 1. Studies on optimization of reagent concentrations and culture condition for enumeration of spot-forming cells" J Immunol Methods. 117. 199-203 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akatsuka J.: "Indication and prognosis of splenectomy of various kinds of diseases in children" Jap J of Pediatr Surgery. 21. 131-135 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akatsuka J.: "Immunoglobulin therapy of children with hematologic diseases" Shinshoniigakutaikei, Nakayama Publisher, Tokyo. 153-167 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchiyama H, Akatsuka J.: "ELIZA-plaque assay for the detection of platelet antibody-secreting cells. Effects of various immunosuppressive treatment on platelet antibody-secreting cells" Jikei Med J. 37. 407-413 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujisawa K, Akatsuka. et al.: "Detection of antigenic determinations of autoantibodies in idiopathic thrombocytopenic purpura using anti-platelet monoclonal antibodies and flow cytometry in whole blood" Rinshoketsueki. 31. 1769-1773 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akatsuka J.: "Recent review of immunoglobulin therapy. -Idiopathic thrombocytopenic thrombocytopenic purpura-" Pediatric Review. 23. 673-691 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikegami M, Fujisawa K, Akatsuka J.: "Platelet associated immunoglobulin in idiopathic thrombocytopenic purpura in childhood. Part 2. The effect of anti-platelet IgG antibody on platelet binding proteins and its morphology."

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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