1989 Fiscal Year Final Research Report Summary
Identification of the region in the hCG cDNA associating its bioactivity by using biotechnology
| Project/Area Number |
63480367
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | OSAKA UNIVERSITY |
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Principal Investigator |
TANIZAWA Osamu OSAKA UNIVERSITY MEDICAL SCHOOL PROFESSOR, 医学部, 教授 (30028443)
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| Co-Investigator(Kenkyū-buntansha) |
KOYAMA Masayasu OSAKA UNIVERSITY MEDICAL SCHOOL CLINICAL FELLOW, 医学部, 助手 (00183351)
AZUMA Chihiro OSAKA UNIVERSITY MEDICAL SCHOOL CLINICAL FELLOW, 医学部, 助手 (20151061)
SAJI Fumitaka OSAKA UNIVERSITY MEDICAL SHOOL ASSISTANT PROFESSOR, 医学部, 講師 (90093418)
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| Project Period (FY) |
1988 – 1989
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| Keywords | HCG / Beta subunit / CAGY region / Invitro metagenesis / Invitro transcription / Invitro translation / Xenopus Oocytes / Gly→Arg、Asp変換 |
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| Research Abstract |
The heterodimeric glycoprotein hormones, hCG, LH, TSH and FSH consist of two noncovalently linked subunits, the alpha and beta subunits. The beta subunit is specific for each hormone and is responsible for the biological specificity, but the beta subunits of different hormones show some degree of structural homology. The CAGY (cysteine-alanine-glycine-tyrosine) region is one of the amino acid sequences that is homologous in different beta subunits and is highly conserved beyond species. In the present study, we used site-specific in vitro mutagenesis to change three individual nucleotides in the center of the CAGY region of the hCG beta subunit, and determined the effects of these mutations on hCG production by in vitro transcription and then translation in Xenopus oocytes. The results indicated that the CAGY region, particularly the glycine residue at position 36 in the beta subunit, is essential for the production of hCG. This finding is consistent with previous studies showing that this region is necessary for the biological activity of hTSH.
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[Publications] Chihiro Azuma, K.Miyai, F.Saji, S.Kamiura, Y.Tokugawa, T.Kimura, K.Ohashi, Y.Iijima, T.Kashiwai, T.Hayashizaki, O.Tanizawa.: "Site-specific mutagenesis of human chrionic gonadotropin (hCG) beta subunit ; influence of mutation on hCG production" The journal of Molecular Endocrinology 1990.
Description
「研究成果報告書概要(欧文)」より
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