1989 Fiscal Year Final Research Report Summary
Determination of ABO blood group of seminal stain mixed with vaginal secretion using immunoblotting
| Project/Area Number |
63570279
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Kurume University |
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Principal Investigator |
TSUDA Ryouichi Kurume University, School of Medicine, assistant, 医学部, 助手 (20098875)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Blood group typing / Blood group ABO system / Seminal fluid / Vaginal fluid / sexual crime / electrophoresis |
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| Research Abstract |
A purpose of this research is development of a new technique for the determination of each ABO blood group of semen and vaginal fluid in sexual cases.
An ABO blood group glycoprotein, which was designated as "slpha_2-seminoglyco- protein (alpha_2-SGP)" was isolated from human seminal plasma. The anti-alpha_2-SGP sera were prepared by immunizing rabbits with the alpha_2-SGP emulsitied with complete Freund's adjuvant.
A new method for determination of each ABO blood group of semen and vaginal fluid in mixture of both semen and vaginal fluid by SDS-PAG electrophoresis-Immunoblotting using anti-alpha_2-SGP sera, anti-A and anti-B was carried out.
(1) The alpha_2-SGP cannot be detected in vaginal fluid. This result suggests that alpha_2-SGP is a seminal ABO blood group specific glycoprotein.
(2) ABO blood group activity of alpha_2-SGP overlaps with that of vaginal fluid ; therefore each ABO blood group of semen and vaginal fluid could not be distinguished.
Results show alpha_2-SGP to be a useful blood group marker for the determination of ABO blood group of semen. However, this method was found to be unsuitable for use with forensic science case.
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