1990 Fiscal Year Final Research Report Summary
Heterogeneity of Prolactin in Sera and CSF in Prolactinoma Patients
| Project/Area Number |
63570670
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Akita University |
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Principal Investigator |
SAKAMOTO Tetsuya Akita University School of Medicine Assistant Professor, 医学部, 助教授 (00004713)
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| Co-Investigator(Kenkyū-buntansha) |
WAKISAKA Akira Kyorin University School of Medicine Professor, 医学部, 教授 (60006707)
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| Project Period (FY) |
1988 – 1990
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| Keywords | Prolactin / Prolactinoma / Radioimmunoassay / Radioreceptor assay / Bioassay |
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| Research Abstract |
In order to study the heterogeneity of prolactin, sera and cerebrospinal fluid obtained from prolactinoma patients were studied employing gel chromatography, radioimmunoassay, radioreceptor assay and bioassay. Chromatography in sera and cerebrospinal fluid gave three types of prolactin with different molecular weights. Radioreceptor assay was used by using a receptor propagation from bovine ovaries. Bioassay was used by using the rat Nb_2 lymphoma cell line. In sera, there was a good correlation between receptor assay, bioassay and radioimmunoassay.
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