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1990 Fiscal Year Final Research Report Summary

Basic and Clinical Study of Arachidonic Acid Metabolism in Pathogenesis of Nasal Polyps

Research Project

Project/Area Number 63570812
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionKagoshima University

Principal Investigator

SHIMA Tetsuya  Kagoshima University Faculty of Medicine, Assistant Professor, 医学部附属病院, 助手 (30206156)

Co-Investigator(Kenkyū-buntansha) NOBORI Takuo  Kagoshima University Faculty of Medicine, Associate Professor, 医学部, 助教授 (40094164)
OHYAMA Masaru  Kagoshima University Faculty of Medicine, Professor, 医学部, 教授 (70024677)
Project Period (FY) 1988 – 1990
KeywordsNasal polyp / Arachidonic acid metabolites / LTB4 / 12-HETE / 15-HETE / Endothelial cell / Ciliated cell / Aspirine-induced asthma
Research Abstract

Arachidonic acid metabolites in nasal polyps were analyzed. Leukotriene (LT) B4, LTC4, LTD4, LTE4, 5-hydroxyeicosatetraenoic acid (HETE), 12-HETE, 15-HETE, Prostaglandin (PG) E2, 6-keto-PG Fla and thromboxane (TX) B2 were quantitated. Both 12-HETE and 15-HETE were detected dominantly in those metaboites. In addition, the enzyme activities of nasal polyps on ^<14>C-labelled arachidonic acid were measured by radiochromatoscanning. Predominancy of 12-and 15-lipoxygenase activities in the polyps was also noted. It has been considered that LTB4 and 5-HETE promoted the inflammatory cell migration and LTC4, LTD4 and LTE4 facilitated the permiability of the vessels which caused edema occurring in the polyps.
In order to investigate the cytotoxic activity of 12- and 15-HETE on the endothelial cells, we established a cell culture of the endothelial cells derived from nasal polyps and pursued the influence of 12- and 15-HETE on growth of the cultured endothelial cells. However, no significant inhi … More bition of the cell growth was observed. Further examination towards these two metabolites was required because of their predominance in the group of arachidonic acid products in nasal polyps.
Cell culture of ciliated cells were performed to determine the origin of 12-and 15-HETE. ^<14>C-labelled arachidonic acid was used as a substrate. The existence of 12- and 15-lipoxygenase activities in the cultured ciliated cells were recognized. It was conceivable that the ciliary epithelium in nasal polyps produced 12- and 15-HETE.
Nasal polyps accompanied with aspirine-induced asthma were treated by local steroid injection. Remarkable reduction of the size of the polyps was noted. And also, serious decrease of the number of migrated eosinophlic granulocytes was observed in morphological examination. These evidence indicated the characteristic pathogenesis of this type of polyps.
Application of Nd-YAG LASER for polypotomy was attained. The LASER irradiation have no significant influence for arachiconic acid metabolism. Compared to the ordinary surgery, the polypotomy using Nd-YAG LASAR achieved more improvement of clinical symptoms during the long term follow. Less

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 島 哲也: "鼻粘膜のリポキシゲ・ナ-ゼ代謝に関する検討" 耳鼻咽喉科臨床学会誌. 補26. 1-14 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大山 勝: "鼻副鼻腔粘膜病変における小青龍湯の抗炎症・抗アレルギ-作用の検討" 漢方と免疫・アレルギ-. 2. 81-93 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大山 勝: "鼻副鼻腔粘膜の炎症とアラキドン酸・カスケ-ド代謝物" 耳鼻咽喉科臨床学会誌. 81. 481-489 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 島 哲也: "アスピリン喘息と鼻茸" 日本耳鼻咽喉科免疫アレルギ-研究会誌. 7. 54-55 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 島 哲也: "鼻副鼻腔アレルギ-とアラキドン酸カスケ-ド代謝物" 頭頸部自律神経. 2. 68-71 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohyama,Masaru: "Laser polypectomy" Rhinology suppl.8. 35-43 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大山 勝: "鼻茸の病態からみた治療法" 耳鼻咽喉科・頭頸部外科. 62. 1149-1155 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大山 勝: "鼻茸の成因と和漢薬" 現代医療学. 6. 326-364 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大山 勝: "鼻茸(ポリ-プ)" 日本医師会雑誌. 101. KS1-KS2 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 島 哲也: "NdーYAG レ-ザ-サ-ミアの家兎舌組織中アラキドン酸代謝に及ぼす影響に関する研究" 日本ハイパ-サ-ミア誌. 5. 57-65 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大山 勝: "慢性副鼻腔炎の漢方治療" JOHNS. 6. 555-558 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 島 哲也: "鼻茸の成因におけるアラキドン酸代謝物の関与" 鼻科学会誌. 29. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shima, T.: "Lipoxygenase metabolites in chronic inflammation of the nasal mucosa" Practica otologica. suppl. 26. 1-14 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohyama, M.: "Anti-inflammatory and Anti-allergic effects of Syo-seiryuu-to on the pathological mucosa of nose and paranasal sinus" Kanpo and immune-allergy. 2. 81-93 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohyama, M.: "Inflammation of nasal and paranasal sinus mucosa and arachidonic acid metabolites" Practica otologica. 81. 481-489 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shima, T.: "Aspirine-induced asthma and nasal polyps" Journal of Japan Society of Immunology and allergology in Otolaryngology. 7. 54-55 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shima, T.: "Nose and paranasal allergy and arachidonic acid cascade metabolites" Head and neck autonomic nervous system. 2. 68-71 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohyama, M.: "Laser polypectomy" Rhinology. suppl. 8. 35-43 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohyama, M.: "Pathogenesis and treatment of nasal polyps" Otolaryngology-Head and neck Surgery. 62. 1149-1155 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohyama, M.: "Pathogenesis of nasal polyps and Wakan-yaku" Gendaiiryou. 6. 326-364 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohyama, M.: "Nasal polyps" The Journal of the Japan medical association. 101. 1-2 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shima, T.: "The effect of hyperthermia with Nd-YAG laser to arachidonic acid metabolites in the rabbit tongues" Jpn. J. Hyperthermic Oncol.5. 57-65 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohyama, M.: "Kampo-treatment of chronic sinusitis" JOHNS. 6. 555-558 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shima, T.: "Arachidonic acid metabolites in pathogenesis of nasal polyps" Jpn. Rhinology. 29. (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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