1989 Fiscal Year Final Research Report Summary
DEVELOPMENT OF BIOSENSORS FOR DETERMINING ENZYME ACTIVITIES
| Project/Area Number |
63860026
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | TOKYO UNIVERSITY OF FISHERIES |
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Principal Investigator |
NAGAYAMA Fumio TOKYO UNIVERSITY OF FISHERIES, DEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY, PROFESSOR, 水産学部, 教授 (90017020)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Etsuo TOKYO UNIVERSITY OF FISHERIES DEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY ASSOCIAT, 水産学部, 助教授 (00017055)
IKEDA Yayoi TOKYO UNIVERSITY OF FISHERIES DEPARTMENT OF AQUATIC BIOSCIENCES PROFESSOR, 水産学部, 教授 (70017078)
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| Project Period (FY) |
1988 – 1989
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| Keywords | Biosensor / Enzyme activity / GOT / GPT / LDH / Serum |
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| Research Abstract |
Several biosensor systems for determining enzyme activities have been developed. 1. Lactate dehydrogenase (lactate + NAD - pyruvate + NADH) was determined with the oxygen electrode on which immobilized pyruvate oxidase (pyruvate + O_2 + H_20 -> acetyl phosphate + CO_2 + H_2O_2)membrane was equipped. 2. Activity of glutamate-pyruvate transaminase(GPT; alanine + oxoglutarate -> pyruvate + glutamate) was determined with the same electrode. 3. A dual enzyme sensor system was devised for simultaneous determination of lactate dehydrogenase and glutamate-pyruvate transaminase. 4. Activity of glutamate-oxaloacetate transaminase (GOT; aspartate + oxoglutarate -> oxaloacetate + glutamate) was determined with the oxygen electrode on which immobilized oxaloacetate decarboxylase (oxaloacetate -> pyruvate + CO_2) and pyruvate oxidase membrane was equipped. T hese sensor systems may be applicable for diagnostic determination of enzymes in fish sera.
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