2019 Fiscal Year Annual Research Report
Role of DNA topology in gene expression.
Publicly Offered Research
| Project Area | Chromatin potential for gene regulation |
|---|
| Project/Area Number |
19H05261
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
Canela Andres 京都大学, 白眉センター, 特定准教授 (90837585)
|
|---|
| Project Period (FY) |
2019-04-01 – 2021-03-31
|
| Keywords | DNA Topoisomerases / Transcription / DNA torsion / RNA polymerase 2 / Chromatin loop |
|---|
| Outline of Annual Research Achievements |
During the fiscal year 2019, I set up a single-cell system to measure the dynamics of transcription activation. Based on previous genomic data I selected genes that activate transcription upon interferon beta treatment in mouse pre-B cells and use a combination of genomics and single-cell imaging changes to monitor transcription. In addition, I have generated cell lines that are deficient for Topoisomerases to evaluate in the second fiscal year the consequences in the dynamics of transcription.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
During the fiscal year 2019:
(1)Setup of a single-cell system to measure the impact of torsional stress in transcription. I have made significative advances in this aim. In addition, (2) I have generated cell lines that will be used during the second year and will allow me to analyze the role of topoisomerase activity in the dynamics of transcription.
|
| Strategy for Future Research Activity |
During the fiscal year 2020, I will use Nascent RNA FISH in combination with ChIP-seq for PolII to quantify monitor transcription dynamics (transcription bursting and intensity of transcription, and the rates of PolII binding, and pause release) in cell lines that are deficient for Topoisomerases. This will identify how torsional tension in the chromatin affects transcription and the role of DNA topoisomerases. In addition, I will evaluate how transcription is affected by the presence of abortive topoisomerase complexes.
|
-
-
[Journal Article] 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination.2020
Author(s)
Callen E, Zong D, Wu W, Wong N, Stanlie A, Ishikawa M, Pavani R, Dumitrache LC, Byrum AK, Mendez-Dorantes C, Martinez P, Canela A, Maman Y, Day A, Kruhlak MJ, Blasco MA, Stark JM, Mosammaparast N, McKinnon PJ, Nussenzweig A
-
Journal Title
Molecular Cell
Volume: 77
Pages: 26-38
DOI
Peer Reviewed / Int'l Joint Research
-
[Journal Article] Topoisomerase II-Induced Chromosome Breakage and Translocation Is Determined by Chromosome Architecture and Transcriptional Activity.2019
Author(s)
Canela A, Maman Y, Huang SN, Wutz G, Tang W, Zagnoli-Vieira G, Callen E, Wong N, Day A, Peters JM, Caldecott KW, Pommier Y, Nussenzweig A
-
Journal Title
Molecular Cell
Volume: 75
Pages: 252-266
DOI
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
