International Activities Supporting Group for Nascent Chain Biology

• Project Area: Nascent-chain Biology
• Project/Area Number: 15K21743
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Multi-year Fund
• Review Section: Biological Sciences
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: 田口 英樹 東京工業大学, 科学技術創成研究院, 教授 (40272710)
• Co-Investigator(Kenkyū-buntansha): 稲田 利文 東北大学, 薬学研究科, 教授 (40242812) ; 田中 元雅 国立研究開発法人理化学研究所, 脳神経科学研究センター, チームリーダー (40321781) ; 河野 憲二 奈良先端科学技術大学院大学, 研究推進機構, 特任教授 (50142005) ; 藤木 幸夫 九州大学, 生体防御医学研究所, 特任教授 (70261237)
• Project Period (FY): 2015-11-06 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥52,260,000 (Direct Cost: ¥40,200,000、Indirect Cost: ¥12,060,000); Fiscal Year 2018: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2017: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2016: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000); Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: 新生鎖 / フォールディング / リボソーム / シャペロン / 翻訳 / 品質管理

Outline of Annual Research Achievements

【田口】新生ポリペプチド鎖（新生鎖）が細胞内で合成されてくるようすを直接可視化する新規の手法（NCT）についてコロラド州立大学と共同研究を実施した。このNCT法を用いることで、神経変性疾患に関わる非典型的な翻訳現象であるリピート関連非AUG翻訳の生細胞での可視化に一部成功した。
【河野】XBP1uがリボソームトンネル内で翻訳アレストを起こした時の立体構造をミュンヘン大学との共同研究でクライオ電子顕微鏡を用いて解析した。この結果、一時的な翻訳アレストにおけるリボソームトンネルと新生鎖の相互作用が明らかとなった。
【田中】複雑な複数のヘアピン構造を含むmRNAを光ピンセットで引っ張った結果、予想通りにヘアピン構造が壊れるシグナルがきれいに見られ、実験系を確立させた。また、ヒト翻訳システムを使い、光ピンセットによって1分子の新生ポリペプチド鎖を引っ張ることに成功した。
【稲田】ミュンヘン大学との共同研究により、品質管理RQCによって認識される異常翻訳の実体を解明した(EMBO J, 2019)。さらに新規品質管理因子RQT1が、特異的な翻訳停止状態のリボソームを認識しユビキチン化する反応を試験管内で再現した(NSMB, 2020)。
【藤木】ペルオキシソーム局在性カタラーゼのサイトゾルへの局在化による酸化ストレス応答機構を発見（Stanford大学：Cell Reports 2019）。b)プラスマローゲン合成障害マウスの小脳では、ペルオキシソーム局在性TAのFar1の増加とともに、ミエリン形成が障害される病態発症機構を解明（Wien医科大学：J. Biochem. 2019）。c) TAタンパク質Pex26pのF51P変異によるペルオキシソームタンパク質の輸送低下が聴覚低下・損失の病因となることを見出した(Columbia大学： Cold Spring Harb. Mol. Case Stud. 2019)。

Research Products

• [Int'l Joint Research] Ludwig-Maximilians-Universitat Munchen(ドイツ)
• [Int'l Joint Research] Colorado State University/Stanford University/Columbia University(米国)
• [Int'l Joint Research] Utrecht University(オランダ)
• [Int'l Joint Research] ウイーン医科大学(オーストリア)
• [Int'l Joint Research] University of Munich/University of Dusseldorf(ドイツ)
• [Int'l Joint Research] ユトレヒト大学(オランダ)
• [Int'l Joint Research] CSIR-IMTEC(インド)
• [Int'l Joint Research] スタンフォード大学/コロンビア大学/コロラド州立大学(米国)
• [Int'l Joint Research] Wien医科大学(オーストリア)
• [Int'l Joint Research] ミュンヘン大学(ドイツ)
• [Int'l Joint Research] ジュネーブ大学(スイス)
• [Int'l Joint Research] カリフォルニア大学バークレー校/スタンフォード大学/ハーバード大学(アメリカ)
• [Int'l Joint Research] ケンブリッジ大学(イギリス)
• [Int'l Joint Research] キング・ファイサル専門病院(サウジアラビア)
• [Int'l Joint Research] UCSF/UCバークレー/スタンフォード大学(アメリカ合衆国)
• [Int'l Joint Research] ハイデルベルク大学/ルートヴィヒ・マクシミリアン大学ミュンヘン/マックスプランク研究所(ドイツ)
• [Int'l Joint Research] ヘルシンキ大学(フィンランド)
• [Journal Article] Systematic Identification of Regulators of Oxidative Stress Reveals Non-canonical Roles for Peroxisomal Import and the Pentose Phosphate Pathway (2020)
  • Author(s): Dubreuil, M.M., Morgens, D.W., Okumoto, K., Honsho, M., Contrepois, K., Lee-McMullen, B., Traber, G.M., Sood, R.S., Dixon, S.J., Snyder, M.P., *Fujiki, Y., and *Bassik, M.C.
  • Journal Title: Cell Reports Volume: 30 Pages: 1417-1433
  • DOI: 10.1016/j.celrep.2020.01.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structure and function of Vms1 and Arb1 in RQC and mitochondrial proteome homeostasis (2019)
  • Author(s): Su T, Izawa T, Thoms M, Yamashita Y, Cheng J, Berninghausen O, Hartl FU, Inada T, Neupert W, Beckmann R.
  • Journal Title: Nature Volume: 570 Pages: 538-542
  • DOI: 10.1038/s41586-019-1307-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Collided ribosomes form a unique structural interface to induce Hel2‐driven quality control pathways (2019)
  • Author(s): Ikeuchi, K., Tesina, P., Matsuo, Y., Sugiyama, T., Cheng, J., Saeki, Y., Tanaka, T., Becker, T., Beckmann, R., and Inada, T.
  • Journal Title: The EMBO Journal Volume: 38
  • DOI: 10.15252/embj.2018100276
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural and mutational analysis of the ribosome-arresting human XBP1u (2019)
  • Author(s): Shanmuganathan, V.
  • Journal Title: eLife Volume: 8
  • DOI: 10.7554/elife.46267
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impaired plasmalogen synthesis dysregulates liver X receptor-dependent transcription in cerebellum. (2019)
  • Author(s): Honsho Masanori、Dorninger Fabian、Abe Yuichi、Setoyama Daiki、Ohgi Ryohei、Uchiumi Takeshi、Kang Dongchon、Berger Johannes、Fujiki Yukio
  • Journal Title: J. Biochem. Volume: 166 Pages: 353-361
  • DOI: 10.1093/jb/mvz043
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Large-scale analysis of diffusional dynamics of proteins in living yeast cells using fluorescence correlation spectroscopy (2019)
  • Author(s): Fukuda Takafumi、Kawai-Noma Shigeko、Pack Chan-Gi、Taguchi Hideki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 520 Pages: 237-242
  • DOI: 10.1016/j.bbrc.2019.09.066
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dynamics of the nucleoside diphosphate kinase protein DYNAMO2 correlates with the changes in the global GTP level during the cell cycle of <i>Cyanidioschyzon merolae</i> (2019)
  • Author(s): Imoto, Y., Abe, Y., Okumoto, K., Ohnuma, M., Kuroiwa, H., Kuroiwa, T., and Fujiki, Y.
  • Journal Title: Proc. Jpn. Acad., Ser. B Volume: 95 Issue: 2 Pages: 75-85
  • DOI: 10.2183/pjab.95.007
  • NAID: 130007591938
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A newly identified mutation in the PEX26 gene is associated with a milder type of Zellweger spectrum disorder. (2019)
  • Author(s): Tanaka, A. J., Okumoto, K., Tamura, S., Abe, Y., Hirsch, Y., Deng, L., Ekstein, J., Chung, W. K., and Fujiki Y.
  • Journal Title: Cold Spring Harbor Molecular Case Studies Volume: 5 Pages: 1-16
  • DOI: 10.1101/mcs.a003483
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The cytosolic peroxisome-targeting signal (PTS)-receptors, Pex7p and Pex5pL, are sufficient to transport PTS2 proteins to peroxisomes. (2019)
  • Author(s): Mukai Satoru、Matsuzaki Takashi、Fujiki Yukio
  • Journal Title: Biochim. Biophys. Acta-Mol. Cell Res. Volume: 1866 Pages: 441-449
  • DOI: 10.1016/j.bbamcr.2018.10.006
  • Peer Reviewed / Open Access
• [Journal Article] Molecular basis for diversification of yeast prion strain conformation (2018)
  • Author(s): Ohhashi Yumiko、Yamaguchi Yoshiki、Kurahashi Hiroshi、Kamatari Yuji O.、Sugiyama Shinju、Uluca Boran、Piechatzek Timo、Komi Yusuke、Shida Toshinobu、M?ller Henrik、Hanashima Shinya、Heise Henrike、Kuwata Kazuo、Tanaka Motomasa
  • Journal Title: Proc Natl Acad Sci U S A Volume: 115 Pages: 2389-2394
  • DOI: 10.1073/pnas.1715483115
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Onsite GTP fuellingvia DYNAMO1drives division of mitochondria and peroxisomes (2018)
  • Author(s): Imoto Yuuta、Abe Yuichi、Honsho Masanori、Okumoto Kanji、Ohnuma Mio、Kuroiwa Haruko、Kuroiwa Tsuneyoshi、Fujiki Yukio
  • Journal Title: Nat. Commun. Volume: 9 Pages: 1-12
  • DOI: 10.1038/s41467-018-07009-z
  • Peer Reviewed / Open Access
• [Journal Article] Peroxisome biogenesis deficiency attenuates the BDNF-TrkB pathway-mediated development of cerebellum. (2018)
  • Author(s): Abe Yuichi、Honsho Masanori、Itoh Ryota、Kawaguchi Ryoko、Fujitani Masashi、Fujiwara Kazushirou、Hirokane Masaaki、Matsuzaki Takashi、Nakayama Keiko、Ohgi Ryohei、Marutani Toshihiro、Nakayama Keiichi I、Yamashita Toshihide、Fujiki Yukio
  • Journal Title: Life Sci. Alliance. Volume: 1 Pages: 1-17
  • DOI: 10.26508/lsa.201800062
  • Peer Reviewed / Open Access
• [Journal Article] A newly isolated Pex7-binding, atypical PTS2 protein P7BP2 is a novel dynein-type AAA+ protein (2018)
  • Author(s): Niwa, H., Miyauchi-Nanri, Y., Okumoto, K., Mukai, S., Noi, K., Ogura, T., and Fujiki Y.
  • Journal Title: The Journal of Biochemistry Volume: 164 Pages: 437-447
  • DOI: 10.1093/jb/mvy073
  • NAID: 40021789527
  • Peer Reviewed / Open Access
• [Journal Article] A LEA model peptide protects the function of a red fluorescent protein in the dry state (2018)
  • Author(s): Furuki T, Niwa T, Taguchi H, Hatanaka R, Kikawada T, *Sakurai M
  • Journal Title: Biochem Biophys Rep Volume: 17 Pages: 27-31
  • Peer Reviewed
• [Journal Article] Electrostatic interactions between middle domain motif-1 and the AAA1 module of the bacterial ClpB chaperone are essential for protein disaggregation. (2018)
  • Author(s): Sugita S, Watanabe K, Hashimoto K, Niwa T, Uemura E, Taguchi H, *Watanabe YH
  • Journal Title: J Biol Chem Volume: 293 Pages: 19228-19239
  • Peer Reviewed
• [Journal Article] The absence of thioredoxin m1 and thioredoxin C in Anabaena sp. PCC 7120 leads to oxidative stress. (2018)
  • Author(s): Deschoenmaeker F, Mihara S, Niwa T, Taguchi H, Wakabayashi KI, *Hisabori T.
  • Journal Title: Plant Cell Physiol. Volume: 59 Pages: 2432-2441
  • Peer Reviewed
• [Journal Article] Large-scale aggregation analysis of eukaryotic proteins reveals an involvement of intrinsically disordered regions in protein folding (2018)
  • Author(s): Uemura E, Niwa T, Minami S, Takemoto K, Fukuchi S, Machida K, Imataka H, Ueda T, Ota M, Taguchi H.
  • Journal Title: Sci Rep Volume: 8 Pages: 678-678
  • DOI: 10.1038/s41598-017-18977-5
  • Peer Reviewed / Open Access
• [Journal Article] Genome-Wide Translation Profiling by Ribosome-Bound tRNA Capture (2018)
  • Author(s): チェンウェン チェン、田中元雅
  • Journal Title: Cell Reports Volume: 23 Pages: 608-621
  • DOI: 10.1016/j.celrep.2018.03.035
  • Peer Reviewed / Open Access
• [Journal Article] New splicing variants of mitochondrial Rho GTPase-1 (Miro1) transport peroxisomes (2018)
  • Author(s): Okumoto, K., Ono, T., Toyama, R., Shimomura, A., Nagata, A., and Fujiki Y.
  • Journal Title: The Journal of Cell Biology Volume: 217 Pages: 619-633
  • DOI: 10.1083/jcb.201708122
  • Peer Reviewed / Open Access
• [Journal Article] ペルオキシソームの恒常性と生理機能制御 (2018)
  • Author(s): 本庄雅則, 藤木幸夫
  • Journal Title: 生化学 Volume: 90
  • Peer Reviewed
• [Journal Article] Identification and functional study of the endoplasmic reticulum stress sensor IRE1 in Chlamydomonas reinhardtii (2018)
  • Author(s): Yasuyo Yamaoka
  • Journal Title: The Plant Journal Volume: 94巻 Pages: 91-104
  • DOI: 10.1111/tpj.13844
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Reduction of Ether-Type Glycerophospholipids, Plasmalogens, by NF-κB Signal Leading to Microglial Activation (2017)
  • Author(s): Hossain, Md. S., Abe, Y., Ali, F., Youssef, M., Honsho, M., Fujiki, Y., and *Katafuchi, T.
  • Journal Title: The Journal of Neuroscience Volume: 37 Pages: 4074-4092
  • DOI: 10.1523/jneurosci.3941-15.2017
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] BAK regulates catalase release from peroxisomes (2017)
  • Author(s): *Fujiki, Y., Miyata, N., Mukai, S., Okumoto, K., Cheng, E. H.
  • Journal Title: Molecular & Cellular Oncology Volume: -
  • DOI: 10.1080/23723556.2017.1306610
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional Complementation (2017)
  • Author(s): *Fujiki, Y.
  • Journal Title: eLS Volume: - Pages: 1-6
  • DOI: 10.1002/9780470015902.a0005676.pub3
  • Peer Reviewed / Open Access
• [Journal Article] Plasmalogen homeostasis: regulation of plasmalogen biosynthesis and its physiological consequence in mammals. (2017)
  • Author(s): Honsho Masanori、Fujiki Yukio
  • Journal Title: .FEBS Lett. Volume: 591 Pages: 2720-2729
  • DOI: 10.1002/1873-3468.12743
  • Peer Reviewed / Open Access
• [Journal Article] Deficiency of a retinal dystrophy protein, Acyl-CoA Binding Domain-containing 5 (ACBD5), impairs peroxisomal β-oxidation of very-long-chain fatty acids. (2017)
  • Author(s): Yagita, Y., Shinohara, K., Liu, Y., and *Fujiki, Y.
  • Journal Title: J Biol Chem. Volume: 292 Pages: 691-705
  • DOI: 10.1074/jbc.m116.760090
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The VDAC2-BAK axis regulates peroxisomal membrane permeability (2017)
  • Author(s): Hosoi, K., Miyata, N., Mukai, S., Furuki, S., Okumoto, K., Cheng, E. H., and *Fujiki, Y.
  • Journal Title: J Cell Biol. Volume: 216 Pages: 709-22
  • DOI: 10.1083/jcb.201605002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Defective ATG16L1-mediated removal of IRE1α drives Crohn’s disease-like ileitis (2017)
  • Author(s): Tschurtschenthaler, M., Adolph, T.E., Ashcroft, J.W., Niederreiter, L., Bharti, R., Saveljeva, S., Bhattacharyya, J., Flak, M.B., Shih, D.Q., Fuhler, G.M., Parkes, M., Kohno, K., Iwawaki, T., van der Woude, C.J., Harding, H., Smith, Peppelenbosch, M.P., Targan, S.R., Ron, D., Rosenstiel, P., Blumberg, R.S., Kaser, A.
  • Journal Title: The Journal of Experimental Medicine Volume: 214 Pages: 401-422
  • DOI: 10.1084/jem.20160791
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing. (2016)
  • Author(s): Chadani Y, Niwa T, Chiba S, Taguchi, H., Ito K.
  • Journal Title: Proc Natl Acad Sci USA Volume: 113
  • DOI: 10.1073/pnas.1520560113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] XBP1u translational pausing requires the interaction between own nascent chain and uL4 (2019)
  • Author(s): 大古殿 美加
  • Organizer: 第71回日本細胞生物学会
• [Presentation] 重複機能ドメインの解析から得られたXBP1遺伝子の起源と進化 (2019)
  • Author(s): 小池 雅昭
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] XBP1u翻訳休止におけるリボソームタンパク質uL4の関与 (2019)
  • Author(s): 大古殿 美加
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] ヒト細胞小胞体機能の低下を鋭敏に検出するレポーターの開発と応用 (2019)
  • Author(s): 八巻 聡
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Peroxisome homeostasis: Tightly regulated import and export of matrix enzyme, catalase (2019)
  • Author(s): Yukio Fujiki
  • Organizer: EMBO Workshop “Current advances in protein translocation across membranes”,2019,,1,1
  • Int'l Joint Research / Invited
• [Presentation] Pathological mechanism of peroxisome biogenesis disorders (2019)
  • Author(s): Yuichi Abe, Masanori Honsho, Ryota Itoh, Ryoko Kawaguchi, Masashi Fujitani, Kazushirou Fujiwara, Masaaki Hirokane, Takashi Matsuzaki, Keiko Nakayama, Ryohei Ohgi, Toshihiro Marutani, Keiichi I. Nakayama, Toshihide Yamashita, and Yukio Fujiki
  • Organizer: EMBO Workshop “Current advances in protein translocation across membranes”
  • Int'l Joint Research
• [Presentation] General role of ribosome ubiquitination in quality controls induced by ribosome stalling (2018)
  • Author(s): Toshifumi Inada
  • Organizer: International Symposium on “Proteins; from the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] Peroxisome homeostasis: Tightly regulated import and export of matrix enzyme, catalase (2018)
  • Author(s): Yukio Fujiki
  • Organizer: International Symposium on “Proteins; from the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] Biogenesis of peroxisomal tail-anchored membrane proteins, new splicing variants of Miro1, acyl-CoA binding domain containing protein 5 (ACBD5), and peroxin Pex26p (2018)
  • Author(s): Kanji Okumoto, Yuichi Yagita, Masanori Honsho, Yukio Fujiki
  • Organizer: International Symposium on “Proteins; from the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] Maintenance and de novo formation of mammalian peroxisomes in the absence of membrane assembly peroxin, Pex16p (2018)
  • Author(s): Yuichi Yagita, Yukio Fujiki
  • Organizer: International Symposium on “Proteins; from the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] Peroxisome homeostasis and human disorders (2018)
  • Author(s): Yukio Fujiki
  • Organizer: BIT's 8th Annual World Congress of Molecular & Cell Biology
  • Int'l Joint Research
• [Presentation] Peroxisome biogenesis, dysfunctions, and disorders (2018)
  • Author(s): Yukio Fujiki
  • Organizer: International Conference on Plasmalogen 2018 (ICP 2018)
  • Int'l Joint Research / Invited
• [Presentation] Local GTP fueling via nucleoside diphosphate kinase like protein DYNAMO1 drives division of peroxisome and mitochondrion (2018)
  • Author(s): Yukio Fujiki, Yuuta Imoto, Yuichi Abe, Masanori Honsho, Kanji Okumoto, Mio Ohnuma, Haruko Kuroiwa, Tsuneyoshi Kuroiwa
  • Organizer: ASCB/EMBO 2018 Meeting
  • Int'l Joint Research
• [Presentation] ペルオキシソームの細胞内移動の制御機構 (2018)
  • Author(s): 奥本寛治
  • Organizer: 「新生鎖の生物学」第５回若手ワークショップ
• [Presentation] Pex14欠損マウスにおける小脳形態異常の分子機構 (2018)
  • Author(s): ◯阿部雄一 、本庄雅則 、藤木幸夫
  • Organizer: 平成３０年度日本生化学会九州支部例会,2018
• [Presentation] テイルアンカー型タパク質ACBD5 の 翻訳速度とペルオキシソームへの 輸送・局在化効率の関連性の検討 (2018)
  • Author(s): ○小山桂恵奈、奥本寛治、田村茂彦、藤木幸夫
  • Organizer: 平成３０年度日本生化学会九州支部例会,2018
• [Presentation] ペルオキシソーム形成因子 Pex14p の細胞周期依存的なリン酸化による機能制御 (2018)
  • Author(s): ◯山下昂一郎、田村茂彦、藤木幸夫
  • Organizer: 平成３０年度日本生化学会九州支部例会,2018
• [Presentation] ペルオキシソームの構成酵素および膜タンパク質の局在性と生理機能制御（シンポジウム：深化したオルガネラ研究） (2018)
  • Author(s): 藤木幸夫
  • Organizer: 第91回日本生化学会大会
• [Presentation] シンポジウム：「New aspects of lipid homeostasis: Regulation of cholesterol and plasmalogen homeostasis and physiological consequences」コレステロールとプラスマローゲンのホメオスタシス機構と疾患 (2018)
  • Author(s): 藤木幸夫、佐藤隆一郎
  • Organizer: 第91回日本生化学会大会京都.,2018
• [Presentation] Plasmalogen homeostasis links to cholesterol biosynthesis in brain.[1S08m-05] プラスマローゲン依存的なコレステロール生合成調節とその障害による病態 (2018)
  • Author(s): Masanori Honsho○本庄 雅則,藤木 幸夫
  • Organizer: 第91回日本生化学会大会,京都.,2018
• [Presentation] ペルオキシソーム形成因子 Pex14p の細胞周期依存的なリン酸化による機能制御メカニズムの解析 (2018)
  • Author(s): ◯山下昂一郎、田村茂彦、藤木幸夫
  • Organizer: 第94回日本生化学会大会,京都.
• [Presentation] 過酸化水素分解酵素カタラーゼの細胞内局在制御による酸化ストレス応答の分子機構 (2018)
  • Author(s): ○藤木 幸夫, 奥本 寛治, 宮田 暖, Emily Cheng
  • Organizer: 第13回臨床ストレス応答学会大会
• [Presentation] 新しく単離したPex7結合PTS2タンパク質P7BP2は新規ダイニンタイプAAA+である(ワークショップ：AAA+ ATPaseリングが織りなす多彩な細胞機能とそのしくみVersatile cellular and molecular functions of AAA+ ATPase rings), (2018)
  • Author(s): ○藤木 幸夫, 丹羽 一, 宮内(南里) 康弘, 奥本 寛治, 向井 悟, 野井 健太郎, 小椋 光, 遠藤 斗志也
  • Organizer: 第41回日本分子生物学会年会,2018
• [Presentation] ペルオキシソーム局在性テイルアンカー型膜タンパク質の輸送局在化機構（ワークショップ： 新生鎖オルガネラ膜タンパク質局在化と品質管理Nascent chain polypeptides of organelle proteins: selectivetransport and quality control）, (2018)
  • Author(s): ○奥本 寛治, 田村 茂彦, 八木田 悠一, 本庄 雅則, 藤木 幸夫
  • Organizer: 第41回日本分子生物学会年会,2018
• [Presentation] ワークショップ： 新生鎖オルガネラ膜タンパク質局在化と品質管理 Nascent chain polypeptides of organelle proteins: selective transport and quality control (2018)
  • Author(s): 藤木 幸夫、川原 裕之
  • Organizer: 第41回日本分子生物学会年会,2018
• [Presentation] 酵母プリオン株におけるタンパク質の凝集と抗凝集 (2018)
  • Author(s): 田中元雅
  • Organizer: 第18回 日本蛋白質科学会・新潟2018
  • Invited
• [Presentation] Understanding Prion Biology in Yeast (2018)
  • Author(s): Motomasa Tanaka
  • Organizer: APPS2018
  • Int'l Joint Research / Invited
• [Presentation] Molecular basis for diversification of yeast prion strain conformationand phenotype, (2018)
  • Author(s): Motomasa Tanaka
  • Organizer: The 23rd Taiwan Biophysics Conference,2018,,1,1
  • Int'l Joint Research / Invited
• [Presentation] The role and mechanism of XBP1u-translational pausing in the ER stress signaling (2018)
  • Author(s): Kenji Kohno
  • Organizer: EMBO Workshop
  • Int'l Joint Research
• [Presentation] 新生鎖によるリボソーム制御の分子機構から新規翻訳産物の生理機能へ (2018)
  • Author(s): 田口英樹
  • Organizer: 第41回 日本分子生物学会年会 ワークショップ
  • Invited
• [Presentation] “Nascent chain-induced ribosome dynamics regulation.” (2018)
  • Author(s): Hideki Taguchi
  • Organizer: International Symposium on Proteins: From the Cradle to the Grave, August 26-29, 2018,
  • Int'l Joint Research
• [Presentation] 翻訳途上の新生ポリペプチド鎖が引き起こす翻訳速度調節、翻訳の途中終了 (2018)
  • Author(s): 田口英樹
  • Organizer: 第18回日本蛋白質科学会年会ワークショップ「拡大する蛋白質の世界：Anfinsenのドグマを超えて」
  • Invited
• [Presentation] Intrinsic ribosome destabilization underlies translation and provides an organism with a strategy of environmental sensing. (2018)
  • Author(s): Hideki Taguchi
  • Organizer: Cold Spring Harbor Laboratory meeting on Protein Homeostasis in Health and Diseases., April 17-21, 2018
  • Int'l Joint Research / Invited
• [Presentation] Lab overview; Drawer data (2018)
  • Author(s): Yukio Fujiki
  • Organizer: New Horizons in Peroxisome Biology
  • Int'l Joint Research / Invited
• [Presentation] Pex5-Ub化によるTraffic制御とストレス応答 (2018)
  • Author(s): 藤木 幸夫
  • Organizer: 第１回ユビキチン研究会
• [Presentation] Core components of peroxisomal membrane translocator of matrix proteins (2017)
  • Author(s): Yukio Fujiki, Yuko Kawamura,and Shigehiko Tamura
  • Organizer: EMBO Conference: Protein translocation and cellular homeostasis
  • Place of Presentation: Hotel Valamar Lacroma (Dubrovnik, Croatia)
  • Year and Date: 2017-03-18
  • Int'l Joint Research
• [Presentation] Bidirectional conversion of chaperonin dependency in the obligate chaperonin substrates (2017)
  • Author(s): Hideki Taguchi
  • Organizer: IPR Seminar/RIKEN Symposium/MEXT Nascent Chain Biology "New Frontiers in Protein Misfolding and Aggregation"
  • Place of Presentation: 大阪大学 蛋白質研究所（吹田市）
  • Year and Date: 2017-01-26
  • Int'l Joint Research
• [Presentation] Genome-wide translation profiling by ribosome-bound tRNA capture (2017)
  • Author(s): Motomasa Tanaka
  • Organizer: International Symposium on
  • Int'l Joint Research
• [Presentation] Abnormal local translation in dendrites impairs cognitive functions in neuropsychiatric disorders (2017)
  • Author(s): Motomasa Tanaka
  • Organizer: The 12th Uehara international symposium
  • Int'l Joint Research / Invited
• [Presentation] Peroxisome Homeostasis, Dysfunctions, and Disorders (2017)
  • Author(s): Yukio Fujiki
  • Organizer: 8th International Conference and Exhibition on
  • Int'l Joint Research / Invited
• [Presentation] Peroxisome homeostasis: Protein translocation and quality control (2017)
  • Author(s): Yukio Fujiki
  • Organizer: 2017 Nascent Chain Biology International Symposium
  • Int'l Joint Research / Invited
• [Presentation] VDAC2-BAK axis regulates peroxisomal membrane permeability and catalase release (2017)
  • Author(s): Yukio Fujiki, Non Miyata, Satoru Mukai, Kenichiro Hosoi, Kanji Okumoto, and Emily H. Cheng
  • Organizer: Gordon Research Conference on Organellar Channels & Transporters
  • Int'l Joint Research / Invited
• [Presentation] Bcl-2 ファミリータンパク質 BAK を介したペルオキシソーム膜透過性制御 (2017)
  • Author(s): 細井謙一郎, 宮田暖, 向井悟, 奥本寛治, Emily Cheng, 藤木幸夫
  • Organizer: New Horizons in Peroxisome Biology
• [Presentation] 過酸化水素分解酵素カタラーゼによる新たな酸化ストレス応答機構の発見 - アポトーシス因子BAKの標的膜依存的な死と生の振り分け - (2017)
  • Author(s): Yukio Fujiki, Non Miyata, Satoru Mukai, Kanji Okumoto, and Emily H. Cheng
  • Organizer: 第69回日本細胞生物学会大会
• [Presentation] プラスマローゲン生合成機構:acyl/alkyl dihydroxyacetonephosphate還元酵素(DHRS7B)のペルオキシソームと小胞体への標的化機構 (2017)
  • Author(s): 田中恵美, 本庄雅則, 藤木幸夫
  • Organizer: 第69回日本細胞生物学会大会
• [Presentation] NDR2 のペルオキシソーム膜への局在と一次繊毛形成における役割 (2017)
  • Author(s): 増川萌瑛 , 阿部彰子, 永井友朗, 奥本寛治, 本間悠太, 藤木幸夫, 水野健作
  • Organizer: 第69回日本細胞生物学会大会
• [Presentation] エーテルリン脂質プラスマローゲンの生合成を制御するセンシング機構 (2017)
  • Author(s): 本庄雅則, 阿部雄一, 藤木幸夫
  • Organizer: 第59回日本脂質生化学会大会
• [Presentation] ペルオキソソームの恒常性：膜およびマトリックスタンパク質輸送の分子基盤 (2017)
  • Author(s): Yukio Fujiki, Kanji Okumoto, Yuichi Yagita, and Shigehiko Tamura
  • Organizer: 第17回日本蛋白質科学会年会
• [Presentation] 過酸化水素分解酵素カタラーゼによる新たな酸化ストレス応答機構の発見 (2017)
  • Author(s): 藤木幸夫, 細井謙一郎, 宮田暖, 向井悟, 奥本寛治, Emily Cheng.
  • Organizer: 第12回臨床ストレス応答学会大会
• [Presentation] ペルオキシソームの機能と細胞内配置・運動性の新たな制御機構 (2017)
  • Author(s): 奥本 寛治、小野 立晃、外山 隆介、細井 謙一郎、宮田 暖、向井 悟、Emily H. Cheng、藤木 幸夫
  • Organizer: 2017年度 生命科学系学会合同年次大会
• [Presentation] エーテルリン脂質プラスマローゲンの恒常性維持機構とその生理的意義 (2017)
  • Author(s): 本庄 雅則、藤木 幸夫
  • Organizer: 2017年度 生命科学系学会合同年次大会
• [Presentation] PEX14障害マウスにおける小脳形態異常 (2017)
  • Author(s): 阿部 雄一、本庄 雅則、大城 遼平、藤木 幸夫
  • Organizer: 2017年度 生命科学系学会合同年次大会
• [Presentation] ペルオキシソーム膜透過装置複合体の同定と輸送メカニズムの解明 (2017)
  • Author(s): 田村 茂彦、河村 優子、藤木 幸夫
  • Organizer: 2017年度 生命科学系学会合同年次大会
• [Presentation] Molecular mechanism of XBP1u translational pausing (2017)
  • Author(s): Miku Ofurudono
  • Organizer: EMBO conference
  • Int'l Joint Research
• [Presentation] Translational pausing in the delivery of XBP1u mRNA to the ER (2017)
  • Author(s): Kenji Kohno
  • Organizer: FASEB summer conference
  • Int'l Joint Research / Invited
• [Presentation] Translational pausing in the ER stress signaling (2017)
  • Author(s): 河野 憲二
  • Organizer: International Symposium on Protein Quality Control
  • Int'l Joint Research / Invited
• [Presentation] Conversion of a chaperonin GroEL-independent protein into an obligate substrate, and vice versa (2016)
  • Author(s): Hideki Taguchi
  • Organizer: The Third International Symposium on Protein Folding and Dynamics
  • Place of Presentation: National Centre for Biological Sciences(NCBS)（Bangalore, India）
  • Year and Date: 2016-11-08
  • Int'l Joint Research
• [Presentation] Peroxisome Homeostasis, Dysfunctions, and Disorders (2016)
  • Author(s): Yukio Fujiki
  • Organizer: The 1st INTERNATIONAL PLASMALOGEN SYMPOSIUM
  • Place of Presentation: Centennial Hall Kyushu University School of Medicine（Fukuoka）
  • Year and Date: 2016-11-07
  • Int'l Joint Research
• [Presentation] Global analysis of yeast protein aggregations using a reconstituted cell-free translation system. (2016)
  • Author(s): 田口 英樹
  • Organizer: 第14回 酵母国際会議（The 14th International Congress on Yeasts; ICY14）
  • Place of Presentation: 淡路夢舞台国際会議場（淡路市）
  • Year and Date: 2016-09-11
  • Int'l Joint Research
• [Presentation] Mechanism of nascent-chain quality control using a reconstituted cell-free translation system of Escherichia coli (2016)
  • Author(s): Hideki Taguchi
  • Organizer: 新生鎖の生物学 国際シンポジウムNascent Chain Biology
  • Place of Presentation: 富士レークホテル（河口湖）
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Autonomous translational pausing is required for XBP1u mRNA recruitment to the ER via the SRP pathway (2016)
  • Author(s): Satoshi Kanda, Kota Yanagitani, and Kenji Kohno
  • Organizer: International Meeting; Nascent Chain Biology
  • Place of Presentation: Fuji Lake Hotel （山梨県富士河口湖町）
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Ultrastructure and molecular mechanism of Ring-shaped Supramolecular Nanomachinery for Peroxisome Division Revealed by Single Organelle EM Analysis (2016)
  • Author(s): Yuuta Imoto, Yuichi Abe, Masaki Yoshida, Masanori Honsho, Kanji Okumoto, Mio Ohnuma, Haruko Kuroiwa,Tsuneyoshi Kuroiwa, and Yukio Fujiki
  • Organizer: 1st Cyanidioschyzon merolae Symposium
  • Place of Presentation: 東京大学（東京都）
  • Year and Date: 2016-07-04
  • Int'l Joint Research
• [Presentation] Ribosome-associated quality control(RQC)requires the ubiquitylation of the ribosomal protein uS10 and novel RQC-triggering factors (2016)
  • Author(s): 稲田 利文
  • Organizer: RNA2016
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing (2016)
  • Author(s): 田口 英樹
  • Organizer: RNA2016 satellite-symposium
  • Place of Presentation: 京都大学 稲盛ホール（京都市）
  • Year and Date: 2016-06-27
  • Int'l Joint Research
• [Presentation] オルガネラ恒常性とその障害の分子基盤 (2016)
  • Author(s): 藤木幸夫
  • Organizer: H28年度日本生化学会九州支部例会
  • Place of Presentation: 鹿児島大学（鹿児島市）
  • Year and Date: 2016-05-14
  • Int'l Joint Research
• [Presentation] Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing (2016)
  • Author(s): Hideki Taguchi
  • Organizer: Cold Spring Harbor Laboratory Meeting "Protein Homeostasis in Health & Disease"
  • Place of Presentation: Cold Spring Harbor Laboratory（New York, USA）
  • Year and Date: 2016-04-18
  • Int'l Joint Research
• [Presentation] Autonomous translational pausing is required for ER targeting and cytoplasmic splicing of XBP1u mRNA (2016)
  • Author(s): Kenji Kohno
  • Organizer: NAIST International Workshop "New era of pre-mRNA splicing world"
  • Place of Presentation: NAIST, (Ikoma, Japan)
  • Year and Date: 2016-03-14
  • Invited
• [Book] Encyclopedia of Food Chemistry (2019)
  • Author(s): Honsho, M. and *Fujiki, Y.
  • Total Pages: 16
  • Publisher: Elsevier Inc.
  • ISBN: 9780128140451
• [Book] 生化学 特集：「オルガネラの生物機能と疾患における破綻機構」オーガナイザー 伊藤 孝司Journal of Japanese Biochemical Society 90(1): 5-13 (2018),2018,9 (2018)
  • Author(s): 本庄雅則, 藤木幸夫.
  • Total Pages: 9
  • Publisher: 公益社団法人日本生化学会
• [Book] 生体の科学 特集：細胞高次機能をつかさどるオルガネラコミュニケーション「細胞の分子構造と機能―核以外の細胞小器官」,2018,5 (2018)
  • Author(s): 藤木幸夫、奥本寛治、本庄雅則
  • Total Pages: 5
  • Publisher: 医学書院
• [Book] 医学のあゆみ蛋白質代謝医学-構造・機能の研究から臨床応用まで蛋白質の一生（揺籠から墓場まで）「輸送（細胞内物流システム）」 (2018)
  • Author(s): 藤木幸夫、奥本寛治
  • Total Pages: 6
  • Publisher: 医歯薬出版株式会社
• [Book] シャペロンによるプリオン凝集体の形成と伝播の制御、蛋白質代謝医学-構造・機能の研究から臨床応用まで、田中啓二企画、医学のあゆみ (2018)
  • Author(s): 中川幸姫、田中元雅
  • Publisher: 医歯薬出版
• [Book] 蛋白質代謝医学 医学のあゆみ別冊-「新生鎖の生物学：翻訳途上の新生ポリペプチド鎖が積極的に関与する生命現象」 (2018)
  • Author(s): 田口英樹
  • Total Pages: 8
  • Publisher: 医歯薬出版株式会社
• [Book] バイオサイエンスとインダストリー-「終止コドンに依らず翻訳を途中終了させる酸性アミノ酸の連続配列」 (2018)
  • Author(s): 田口英樹、茶谷悠平、千葉志信、伊藤維昭
  • Total Pages: 2
  • Publisher: バイオインダストリー協会
• [Book] 実験医学 36, 5月号-「翻訳途上の新生ポリペプチド鎖が引き起こすリボソームの不安定化とその生理的意義」 (2018)
  • Author(s): 茶谷 悠平、千葉 志信、伊藤 維昭、田口 英樹
  • Total Pages: 3
  • Publisher: 羊土社
• [Book] "実験医学 (2017)
  • Author(s): 藤木幸夫, 山下俊一, 奥本寛治, 本庄雅則
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] Analysis of plasmalogen synthesis in cultured cells In: Schrader, M (ed) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed: Walker, JM) (2017)
  • Author(s): Honsho, M, and Fujiki, Y
  • Total Pages: 7
  • Publisher: Springer, Humana Press, New York, USA
• [Book] Experimental systems to study yeast pexophagy In: Schrader, M (ed) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed: Walker, JM) (2017)
  • Author(s): Yamashita, S, Oku, M, Sakai, Y, and Fujiki, Y
  • Total Pages: 7
  • Publisher: Springer, Humana Press, New York, USA
• [Book] In vitro PMP import analysis using cell-free synthesized PMP and isolated peroxisomes In: Schrader, M (ed) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed: Walker, JM) (2017)
  • Author(s): Liu,Y, Honsho, M, and Fujiki, Y
  • Total Pages: 6
  • Publisher: Springer, Humana Press, New York, USA
• [Book] Generation of peroxisome-deficient somatic animal cell mutants In: Schrader, M (ed) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed: Walker, JM) (2017)
  • Author(s): Okumoto, K, and Fujiki, Y
  • Total Pages: 9
  • Publisher: Springer, Humana Press, New York, USA
• [Book] Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system In: Schrader, M (ed) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed: Walker, JM) (2017)
  • Author(s): Okumoto, K, Honsho, M, Liu, Y, and Fujiki, Y
  • Total Pages: 7
  • Publisher: Springer, Humana Press, New York, USA
• [Book] Assessing pexophagy in mammalian cells In: Schrader, M (ed) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed: Walker, JM) (2017)
  • Author(s): Yamashita, S, and Fujiki, Y
  • Total Pages: 6
  • Publisher: Springer, Humana Press, New York, USA
• [Book] Blue-Native PAGE: Applications to study on peroxisome biogenesis In: Schrader, M (ed) Peroxisomes: Methods and Protocols, Methods in Molecular Biology (Series Ed: Walker, JM) (2017)
  • Author(s): Okumoto, K, Tamura, S, and Fujiki, Y
  • Total Pages: 9
  • Publisher: Springer, Humana Press, New York, USA
• [Book] 別冊・医学のあゆみ ストレスシグナルと疾患―細胞恒常性維持機構の破綻と病態 『ペルオキシソーム形成異常と疾患』 (2016)
  • Author(s): 藤木幸夫, 奥本寛治, 本庄雅則
  • Total Pages: 5
  • Publisher: 医歯薬出版株式会社