秩序形成のロジックの総括研究

• Project Area: From molecules, cells to organs : trans-hierarchical logic for higher-order pattern and structures
• Project/Area Number: 22127001
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Research Institution: The University of Tokyo
• Principal Investigator: 武田 洋幸 The University of Tokyo, 理学(系)研究科(研究院), 教授 (80179647)
• Project Fiscal Year: 2010-06-23 – 2015-03-31(Estimated)
• Project Status: Completed(Fiscal Year 2014)
• Budget Amount: ¥51,480,000 (Direct Cost : ¥39,600,000、Indirect Cost : ¥11,880,000); Fiscal Year 2014 : ¥11,570,000 (Direct Cost : ¥8,900,000、Indirect Cost : ¥2,670,000); Fiscal Year 2013 : ¥10,140,000 (Direct Cost : ¥7,800,000、Indirect Cost : ¥2,340,000); Fiscal Year 2012 : ¥10,660,000 (Direct Cost : ¥8,200,000、Indirect Cost : ¥2,460,000); Fiscal Year 2011 : ¥10,530,000 (Direct Cost : ¥8,100,000、Indirect Cost : ¥2,430,000); Fiscal Year 2010 : ¥8,580,000 (Direct Cost : ¥6,600,000、Indirect Cost : ¥1,980,000)
• Keywords: 器官形成 / 体軸形成 / 数理モデル / 力学的パラメーター / パターン形成 / 力学的パラメータ

Outline of Annual Research Achievements

領域代表の武田および連携研究者6名（計画研究代表）を中心として、平成25年度および平成26年度に繰り越した予算で、以下の活動を行った。
・平成25年度は、6/12-14に、ゲストスピーカーに森島圭祐氏（大阪大学工学研究科）を迎え、兵庫県淡路夢舞台にて班会議を開催し、8/26-27には同じ場所で夏合宿を行い、生物実験と数理モデル化についての密な討論と共同研究の打合せを行った。夏合宿は絞られた研究に関して時間制限無く議論する場であり、生物と理論系研究者との相互理解がさらに深まった。12/3-6に開催された第36回日本分子生物学会年会ではシンポジウムを企画し、Antonio Giraldez (Yale School Medicine) 氏を招へいし、秩序形成のロジックに関する研究発表と情報交換を行った。また発表者3名に対しての旅費支援を行った。2014年1/19-22に香港科技大学で行われた6th Asia Oceania Zebrafish Meeting HKUST, Hong Kongに、代表の武田が出席し研究成果発表を行った。この他、4回のテクニカルセミナーと、研究室間交流のための旅費支援を実施した。
・平成26年12/2-4に、シンガポール・メカノバイオロジー研究所と共催で行われたシンポジウム「Mechanobiology of Development and Multicellular Dynamics」において、計画研究代表2名が研究発表を行ったほか、参加する公募班員と学生計7名に対して旅費支援を実施した。このシンポジウムでは、組織、細胞が発生する力と組織変形の相関、力の実測に関して、最先端の技術と研究手法について密な討論が行われ、領域内の研究がさらに活性化された。
以上のように総括班として、領域内の共同研究の活性化、新技術の導入、成果発表を積極的に支援した。

Current Status of Research Progress

Research has progressed on the whole more than it was originally planned.

Reason

総括班として、班会議、夏合宿、班内の共同研究に関わる旅費支援、シンポジウム参加への支援などを通して、領域内の研究活動が活発化し、研究成果も得られた。

Research Output

• [Journal Article] Left-right asymmetry is formed in individual cells by intrinsic cell chirality (2014)
  • Author(s): Hatori, R., Ando, T., Sasamura, T., Nakazawa, N., Nakamura, M., Taniguchi, K., Hozumi, S., Kikuta, J., Ishii, M., *Matsuno, K.
  • Journal Title: Mech Dev Volume : 133 Pages : 482-95
  • DOI: S0012-1606(13)00391-6 [pii]10.1016/j.ydbio.2013.07.023
• [Journal Article] Estimation of the mechanical connection between apical stress fibers and the nucleus in vascular smooth muscle cells cultured on a substrate (2014)
  • Author(s): Nagayama K, Yamazaki S, Yahiro Y, Matsumoto T.
  • Journal Title: J Biomechanics Volume : 47 Pages : 1422-29
  • DOI: S0021-9290(14)00073-6 [pii]10.1016/j.jbiomech.2014.01.042
• [Journal Article] In vitro analysis suggests that difference in cell movement during direct interaction can generate various pigment patterns in vivo (2014)
  • Author(s): Yamanaka H, Kondo S
  • Journal Title: PNAS Volume : 111 Pages : 1867-72
  • DOI: 1315416111 [pii]10.1073/pnas.1315416111
• [Journal Article] Involvement of Delta–Notch signaling in zebrafish adult pigment stripe patterning (2014)
  • Author(s): Hamada H, Watanabe M, Lau HE, Nishida T, Hasegawa T, Parichy DM, Kondo S, Parichy DM, Kondo S
  • Journal Title: Development Volume : 141 Pages : 318-24
  • DOI: dev.099804 [pii]10.1242/dev.099804
• [Journal Article] Directional migration of leading-edge mesoderm generates physical forces: Implication in Xenopus notochord formation during gastrulation. (2013)
  • Author(s): Hara Y, Nagayama K, Yamamoto TS, Matsumoto T, Suzuki M, Ueno N
  • Journal Title: Dev Biol Volume : 382 Pages : 482-95
  • DOI: S0012-1606(13)00391-6 [pii]10.1016/j.ydbio.2013.07.023
• [Journal Article] Haemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow-dependent expression of miR-21 (2013)
  • Author(s): Banjo T, Grajcarek J, Yoshino D, Osada H, Miyasaka KY, Kida YS, Ueki Y, Nagayama K, Kawakami K, Matsumoto T, Sato M, Ogura T
  • Journal Title: Nature Commun Volume : 4 Pages : 1978
  • DOI: ncomms2978 [pii]10.1038/ncomms2978
• [Journal Article] Trunk exoskeleton in teleosts is mesodermal in origin (2013)
  • Author(s): Shimada, A., Kawanishi, T., Kaneko, T., Yoshihara, H., Yano, T., Inohaya, K., Kinoshita, M., Kamei, Y., Tamura, K. & Takeda, H.
  • Journal Title: Nat Commun, Volume : 4 Pages : ‐
  • DOI: ncomms2643 [pii]10.1038/ncomms2643
• [Journal Article] Modular development of the teleost trunk along the dorsoventral axis and zic1/zic4 as selector genes in the dorsal module (2013)
  • Author(s): Kawanishi, T., Kaneko, T., Moriyama, Y., Kinoshita, M., Yokoi, H., Suzuki, T., Shimada, A. & Takeda, H.
  • Journal Title: Development Volume : 140 Pages : 1486-96
  • DOI: dev.088567 [pii]10.1242/dev.088567
• [Journal Article] The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution (2012)
  • Author(s): Moriyama, Y.
  • Journal Title: Current Biology Volume : 22 Pages : 601-607
  • DOI: S0960-9822(12)00132-7 [pii]10.1016/j.cub.2012.01.063
• [Journal Article] Pigment pattern formation by contact-dependent depolarization (2012)
  • Author(s): Inaba, M.
  • Journal Title: Science Volume : 335 Pages : 677
  • DOI: 335/6069/677 [pii]10.1126/science.1212821
• [Journal Article] Cell movements of the deep layer of non-neural ectoderm underlie complete neural tube closure in Xenopus (2012)
  • Author(s): Morita, H.
  • Journal Title: Development Volume : 139 Pages : 1417-1426
  • DOI: dev.073239 [pii]10.1242/dev.073239
• [Journal Article] Rescue of Notch signaling in cells incapable of GDP-L-fucose synthesis by gap junction transfer of GDP-L-fucose in Drosophila (2012)
  • Author(s): Ayukawa, T., Matsumoto, K., Ishikawa, HO., Ishino, A., Yamakawa, T., Aoyama, N., Suzuki, T., Matsuno. K.
  • Journal Title: Proceeding of the National Academic Sciences Volume : 109(38) Pages : 15318-15323
  • DOI: 10.1073/pnas.1202369109
• [Journal Article] Changing clothes easily: connexin41.8 regulates skin pattern variation (2012)
  • Author(s): Watanabe, m., Kondo, S.
  • Journal Title: Pigmnet Cell & Melanoma Reserch Volume : 25(3) Pages : 326-330
  • DOI: 10.1111/j.1755-148x.2012.00984.x
• [Journal Article] Deficient Notch signaling associated with neurogenic pecanex is compensated for by the unfolded protein response in Drosophila (2012)
  • Author(s): Yamakawa, T., Yamada, K., Sasamura, T., Nakazawa, N., Kanai, M., Suzuki, E., Fortini, ME., Matsuno, K.
  • Journal Title: Development Volume : 139(3) Pages : 558-567
  • DOI: 10.1242/dev.073858
• [Journal Article] Multiple functions of PBRM-1/Polybromo- and LET-526/Osa-containing chromatin remodeling complexes in C.elegans development (2012)
  • Author(s): Shibata, Y., Uchida, M., Takeshita, H., Nishiwaki, K., Sawa, H.
  • Journal Title: Developmental Biology Volume : 361(2) Pages : 349-357
  • DOI: 10.1016/j.ydbio.2011.10.035
• [Journal Article] The Medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution (2012)
  • Author(s): Moriyama, Y., Nakamura, R., Tsukahara, T., Sumiyama, K., Suster, M.L., Kawakami, K., Toyoda, A., Fujiyama, A., Yasuoka, Y., Nagao, Y., Sawatari, E., Shimizu, A., Wakamatsu, Y., Hibi, M., Taira, M., Okabe, M., Naruse, K., Hashimoto, H., Shimada, A., Takeda, H.
  • Journal Title: Current Biology Volume : 22(7) Pages : 601-607
  • DOI: 10.1016/j.cub.2012.01.063
• [Journal Article] Pigment Pattern Formation by Contact-Dependent Depolarization (2012)
  • Author(s): Inaba, M., Yamanaka, H., Kondo, S.
  • Journal Title: Science Volume : 335 Pages : 677
  • DOI: 10.1126/science.1212821
• [Journal Article] Dynamic Changes of Traction Force at Focal Adhesions during Macroscopic Cell Stretching Using an Elastic Micropillar Substrate: Tensional homeostasis of aortic smooth muscle cells (2012)
  • Author(s): Nagayama, K., Adachi, A., Matsumoto, T.
  • Journal Title: Journal of Biomechanical Science and Engineering Volume : 7(2) Pages : 130-140
  • DOI: 10.1299/jbse.7.130
• [Journal Article] Chirality in planar cell shape contributes to left-right asymmetric epithelial morphogenesis (2011)
  • Author(s): Taniguchi, K.
  • Journal Title: Science Volume : 333 Pages : 339-341
  • DOI: 333/6040/339 [pii]10.1126/science.1200940
• [Journal Article] Wnt regulates spindle asymmetry to generate asymmetric nuclear β-catenin in C. elegans (2011)
  • Author(s): Sugioka, K.
  • Journal Title: Cell Volume : 146 Pages : 942-954
  • DOI: S0092-8674(11)00887-7 [pii]10.1016/j.cell.2011.07.043
• [Journal Article] Heterogeneous Response of Traction Force at Focal Adhesions of Vascular Smooth Muscle Cells Subjected to Macroscopic Stretch on a Micropillar Substrate (2011)
  • Author(s): Nagayama, K.
  • Journal Title: Journal of Biomechanics Volume : 44 Pages : 2699-2705
  • DOI: S0021-9290(11)00545-8 [pii]10.1016/j.jbiomech.2011.07.023
• [Presentation] Cell chirality derives the left-right directional torsions Drosophila gut (2014)
  • Author(s): 松野健二
  • Organizer: MBI-Japan Joint Symposium2014
  • Place of Presentation: National University of Singapore
  • Year and Date: 2014-12-03
• [Presentation] Engineering approaches toward estimation of stress and strain distributions in Xenopus laevis embryos (2014)
  • Author(s): 松本健郎
  • Organizer: MBI-Japan Joint Symposium2014
  • Place of Presentation: National University of Singapore
  • Year and Date: 2014-12-03
• [Presentation] Cell Chirality Induces Mechanical Force Driving the Left-Right Directional Rotations of the Gut in Drosophila (2014)
  • Author(s): 松野健二
  • Organizer: Force in Development
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2014-11-18
• [Presentation] Establishment of robust compartments in fish somites along the dorsoventral axis (2014)
  • Author(s): 武田洋幸
  • Organizer: Force in Development
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2014-11-18
• [Presentation] Biomechanical analyses of Xenopus laevis embryo: Toward estimation of stress and strain distributions during morphogenesis (2014)
  • Author(s): 松本健郎
  • Organizer: Force in Development
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2014-11-18
• [Presentation] Ca2+ dynamics during neural tube formation of Xenopus (2014)
  • Author(s): 上野直人
  • Organizer: Force in Development
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2014-11-17
• [Presentation] Hemodynamic Forces as a Regulator of Cardiogenesis and Circulatory Homeostasis (2014)
  • Author(s): 小椋利彦
  • Organizer: Force in Development
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2014-11-17
• [Presentation] Epijenetic regulation of key developmental genes in the medaka genome: Large DNA hypomethylated domains as a robust repression platform in pluripotent cells (2014)
  • Author(s): 武田洋幸
  • Organizer: 6th Asia oceania Zebrafish Meeting HKUST, Hong Kong
  • Place of Presentation: 香港（中国）
  • Year and Date: 2014-01-21
• [Presentation] 新しいメカノバイオロジーのために‐医学/生物学的意義と融合的研究の重要性‐ (2013)
  • Author(s): 小椋利彦
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2013-12-04
• [Presentation] 平面内細胞極性の数理モデル (2012)
  • Author(s): 秋山正和
  • Organizer: 応用数学合同研究集会2012
  • Place of Presentation: 龍谷大学（滋賀）
  • Year and Date: 20121220-20121222
• [Presentation] From molecules and cells to organs : trans-hierarchical logic for higher-order pattern and structures (2011)
  • Author(s): Takeda, H.
  • Organizer: JSDB-GFE Joint meeting of Developmental Biology
  • Place of Presentation: ドレスデン(ドイツ)
  • Year and Date: 20110323-20110326
• [Presentation] Casting Genetic Light on Morphological Diversity in the Fish Body Shape
  • Author(s): Takeda, H.
  • Organizer: 22nd Pacific Science Congress.
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Presentation] “波”が生み出す多彩な生命機能
  • Author(s): 近藤 滋
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館（松本市）
• [Remarks]
  • URL: http://www.morphologic.jp/
• [Remarks] ミクロからマクロへ階層を超える秩序形成のロジック
  • URL: http://www.morphologic.jp
• [Remarks] ミクロからマクロへ階層を超える秩序形成のロジック
  • URL: http://www.morphologic.jp
• [Remarks] ミクロからマクロへ階層を超える秩序形成のロジック
  • URL: http://www.morphologic.jp/