| Budget Amount *help |
¥79,430,000 (Direct Cost: ¥61,100,000、Indirect Cost: ¥18,330,000)
Fiscal Year 2020: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2019: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2018: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2017: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
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| Outline of Final Research Achievements |
In this study, we investigated the scrap-and-build mechanism of neurons in the brain. Particularly we focused on the elimination mechanism of unwanted compartments in living neurons. We started to analyze the activation mechanism of scramblase Xkr4, which is specifically expressed in neurons. We developed a screening system using CRISPR sgRNA library, which we call revival screening. This system enables identification of factors even in non-proliferating or dying cells, and we identified the nuclear factor XRCC4 as its activation factor. XRCC4 is released into the cytoplasm when cleaved by caspases, and binds to and activates Xkr4. Xkr4 knockout mice were also analyzed and found to exhibit behavioral abnormalities.
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