Control of proton motive force components by ion transport system

• Project Area: New Photosynthesis : Reoptimization of the solar energy conversion system
• Project/Area Number: 16H06558
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: Uozumi Nobuyuki 東北大学, 工学研究科, 教授 (40223515)
• Co-Investigator(Kenkyū-buntansha): 川崎 寿 東京大学, 生物生産工学研究センター, 特任教授 (90349788) ; 池内 昌彦 東京大学, 大学院総合文化研究科, 名誉教授 (20159601)
• Project Period (FY): 2016-06-30 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥85,410,000 (Direct Cost: ¥65,700,000、Indirect Cost: ¥19,710,000); Fiscal Year 2020: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2019: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2018: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2017: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2016: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
• Keywords: 膜輸送体 / 膜電位 / プロトン駆動力 / イオン / チラコイド膜 / 光合成 / イオン輸送体 / 浸透圧 / 恒常性 / 輸送体 / 駆動力 / チャネル / 生体膜 / アンチポーター / 葉緑体 / 藍藻 / カリウム / ナトリウム / イオン透過性

Outline of Final Research Achievements

We showed that Arabidopsis chloroplasts possess both influx and efflux of K-transport activities in KEA1-KEA6. In addition, the mutant, dpgr (KEA3-G422R), exhibited increased spectifc transport activity, compared with that of the wild type KEA3, consistent with physiological results. We also detected the Na and H exchange transport activity of the cyanobacterial Na/H antiporters, NhaS1-NhaS6. Some of NhaS located in the thylakoid membranes, and played an essential role in photosynthetic function under high light conditions.

Academic Significance and Societal Importance of the Research Achievements

光合成の明反応は葉緑体や藍藻で行われており，両者の長所を生かして詳細な検討をすすめた．チラコイド膜におけるプロトン駆動力の形成に必要なNaやK輸送体を同定して，イオン輸送活性を明らかにした．このことにより，チラコイドの膜電位形成には，H以外のNaやKなどの陽イオンが関与しており，その輸送体の実体の一部が明らかにされた．これらの輸送体が光エネルギーの変換でチラコイド膜に蓄積される電気化学エネルギーの生成と変換に重要な役割を担うことが示された．

Research Products

• [Int'l Joint Research] University of Milan(イタリア)
• [Int'l Joint Research] University of Munster(ドイツ)
• [Int'l Joint Research] University of Milan(イタリア)
• [Int'l Joint Research] University of Munster(ドイツ)
• [Int'l Joint Research] University of Munster(ドイツ)
• [Int'l Joint Research] University of Padova(イタリア)
• [Int'l Joint Research] Stockholm University(Sweden)
• [Journal Article] Loss of cell wall integrity genes cpxA and mrcB causes flocculation in Escherichia coli (2021)
  • Author(s): Sugawara Keita、Toyoda Hayato、Kimura Mami、Hayasaka Shunsuke、Saito Hiromi、Kobayashi Hiroshi、Ihara Kunio、Ida Tomoaki、Akaike Takaaki、Ando Eiji、Hyodo Mamoru、Hayakawa Yoshihiro、Hamamoto Shin、Uozumi Nobuyuki
  • Journal Title: Biochemical Journal Volume: 478 Issue: 1 Pages: 41-59
  • DOI: 10.1042/bcj20200723
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• [Journal Article] Biosynthesis of a sulfated exopolysaccharide, Synechan, and bloom formation in the model cyanobacterium Synechocystis sp. strain PCC 6803. (2021)
  • Author(s): Kaisei Maeda, Yukiko Okuda, Gen Enomoto, Satoru Watanabe, Masahiko Ikeuchi
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• [Journal Article] Calcium-Regulated Phosphorylation Systems Controlling Uptake and Balance of Plant Nutrients (2020)
  • Author(s): Saito Shunya、Uozumi Nobuyuki
  • Journal Title: Frontiers in Plant Science Volume: 11 Pages: 1-11
  • DOI: 10.3389/fpls.2020.00044
  • Peer Reviewed / Open Access
• [Journal Article] Functional characterization of multiple PAS domain-containing diguanylate cyclases in Synechocystis sp. PCC 6803 (2020)
  • Author(s): Ishikawa Ko、Chubachi Chihiro、Tochigi Saeko、Hoshi Naomi、Kojima Seiji、Hyodo Mamoru、Hayakawa Yoshihiro、Furuta Tadaomi、Kera Kota、Uozumi Nobuyuki
  • Journal Title: Microbiology Volume: 166 Issue: 7 Pages: 659-668
  • DOI: 10.1099/mic.0.000929
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• [Journal Article] Diverse Physiological Functions of Cation Proton Antiporters across Bacteria and Plant Cells (2020)
  • Author(s): Tsujii Masaru、Tanudjaja Ellen、Uozumi Nobuyuki
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 12 Pages: 4566-4566
  • DOI: 10.3390/ijms21124566
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of Arabidopsis TPK2 and KCO3 reveals structural properties required for K+ channel function (2020)
  • Author(s): Uehara Chihiro、Takeda Kota、Ibuki Tatsuki、Furuta Tadaomi、Hoshi Naomi、Tanudjaja Ellen、Uozumi Nobuyuki
  • Journal Title: Channels Volume: 14 Issue: 1 Pages: 336-346
  • DOI: 10.1080/19336950.2020.1825894
  • Peer Reviewed / Open Access
• [Journal Article] Hik36?Hik43 and Rre6 act as a two-component regulatory system to control cell aggregation in Synechocystis sp. PCC6803 (2020)
  • Author(s): Kera Kota、Yoshizawa Yuichiro、Shigehara Takehiro、Nagayama Tatsuya、Tsujii Masaru、Tochigi Saeko、Uozumi Nobuyuki
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 1-9
  • DOI: 10.1038/s41598-020-76264-2
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• [Journal Article] Tlr0485 is a cAMP-activated c-di-GMP phosphodiesterase in a cyanobacterium <i>Thermosynechococcus</i> (2020)
  • Author(s): Enomoto Gen、Kamiya Ayako、Okuda Yukiko、Narikawa Rei、Ikeuchi Masahiko
  • Journal Title: The Journal of General and Applied Microbiology Volume: 66 Issue: 2 Pages: 147-152
  • DOI: 10.2323/jgam.2020.01.007
  • NAID: 130007855503
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed
• [Journal Article] Guard Cell Membrane Anion Transport Systems and Their Regulatory Components: An Elaborate Mechanism Controlling Stress-Induced Stomatal Closure (2019)
  • Author(s): Saito Shunya、Uozumi Nobuyuki
  • Journal Title: Plants Volume: 8 Issue: 1 Pages: 9-9
  • DOI: 10.3390/plants8010009
  • Peer Reviewed / Open Access
• [Journal Article] The mechanosensitive channel YbdG from Escherichia coli has a role in adaptation to osmotic up-shock. (2019)
  • Author(s): Shun Amemiya, Hayato Toyoda, Mami Kimura, Hiromi Saito, Hiroshi Kobayashi, Kunio Ihara 3 , Kiyoto Kamagata 4 , Ryuji Kawabata 5 , Setsu Kato, Yutaka Nakashimada, Tadaomi Furuta, Shin Hamamoto, Nobuyuki Uozumi
  • Journal Title: Journal of Biological Chemistry Volume: 294 (33) Issue: 33 Pages: 12281-12292
  • DOI: 10.1074/jbc.ra118.007340
  • Peer Reviewed
• [Journal Article] Evidence for potassium transport activity of Arabidopsis KEA1-KEA6. (2019)
  • Author(s): Masaru Tsujii, Kota Kera, Shin Hamamoto, Takashi Kuromori, Toshiharu Shikanai, Nobuyuki Uozumi
  • Journal Title: Scientific Report Volume: 9 (1), 10040 Issue: 1 Pages: 10040-10040
  • DOI: 10.1038/s41598-019-46463-7
  • Peer Reviewed / Open Access
• [Journal Article] DAY-LENGTH-DEPENDENT DELAYED-GREENING1, the Arabidopsis Homolog of the Cyanobacterial H+-Extrusion Protein, Is Essential for Chloroplast pH Regulation and Optimization of Non-Photochemical Quenching (2019)
  • Author(s): Harada Kyohei、Arizono Takatoshi、Sato Ryoichi、Trinh Mai Duy Luu、Hashimoto Akira、Kono Masaru、Tsujii Masaru、Uozumi Nobuyuki、Takaichi Shinichi、Masuda Shinji
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 12 Pages: 2660-2671
  • DOI: 10.1093/pcp/pcz203
  • Peer Reviewed
• [Journal Article] Improved sorbitol production and growth in cyanobacteria using promiscuous haloacid dehalogenase-like hydrolase (2019)
  • Author(s): Chin Taejun、Okuda Yukiko、Ikeuchi Masahiko
  • Journal Title: Journal of Biotechnology: X Volume: 1 Pages: 100002-100002
  • DOI: 10.1016/j.btecx.2019.100002
  • Peer Reviewed / Open Access
• [Journal Article] Cesium Inhibits Plant Growth Primarily Through Reduction of Potassium Influx and Accumulation in Arabidopsis (2018)
  • Author(s): Adams Eri、Miyazaki Takae、Saito Shunya、Uozumi Nobuyuki、Shin Ryoung
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 1 Pages: 63-76
  • DOI: 10.1093/pcp/pcy188
  • Peer Reviewed
• [Journal Article] N-myristoylation and S-acylation are common modifications of Ca2+-regulated Arabidopsis kinases and are required for activation of the SLAC1 anion channel (2018)
  • Author(s): Saito Shunya、Hamamoto Shin、Moriya Koko、Matsuura Aiko、Sato Yoko、Muto Jun、Noguchi Hiroto、Yamauchi Seiji、Tozawa Yuzuru、Ueda Minoru、Hashimoto Kenji、Koester Philipp、Dong Qiuyan、Held Katrin、Kudla Joerg、Utsumi Toshihiko、Uozumi Nobuyuki
  • Journal Title: New Phytologist Volume: 218 Issue: 4 Pages: 1504-1521
  • DOI: 10.1111/nph.15053
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In vitro and in vivo characterization of modulation of the vacuolar cation channel TRPY1 from Saccharomyces cerevisiae (2018)
  • Author(s): Shin Hamamoto, Yasuo Mori, Isamu Yabe, Nobuyuki Uozumi
  • Journal Title: The Febs Journal Volume: 285 Issue: 6 Pages: 1146-1161
  • DOI: 10.1111/febs.14399
  • Peer Reviewed
• [Journal Article] Reduction of Spermidine Content Resulting from Inactivation of Two Arginine Decarboxylases Increases Biofilm Formation in Synechocystis sp. PCC 6803 (2018)
  • Author(s): Kota Kera, Tatsuya Nagayama, Kei Nanatani, Chika Saeki-Yamoto, Akira Tominaga, Satoshi Souma, Nozomi Miura, Kota Takeda, Syunsuke Kayamori, Eiji Ando, Kyohei Higashi, Kazuei Igarashi and Nobuyuki Uozumi
  • Journal Title: Journal of Bacteriology Volume: 9 Issue: 9 Pages: 664-706
  • DOI: 10.1128/jb.00664-17
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• [Journal Article] Measurement of the mechanical properties of single Synechocystis sp. strain PCC6803 cells in different osmotic concentrations using a robot-integrated microfluidic chip (2018)
  • Author(s): Di Chang, Shinya Sakuma, Kota Kera, Nobuyuki Uozumi, Fumihito Arai
  • Journal Title: Lab on a Chip Volume: 8 Issue: 8 Pages: 1241-1249
  • DOI: 10.1039/c7lc01245d
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• [Journal Article] Identification and characterization of compounds that affect stomatal movements. (2018)
  • Author(s): Toh S, Inoue S, Toda Y, Yuki T, Suzuki K, Hamamoto S, Fukatsu K, Aoki S, Uchida M, Asai E, Uozumi N, Sato A, Kinoshita T.
  • Journal Title: Plant & Cell Physiology Volume: in press Issue: 8 Pages: 1568-1580
  • DOI: 10.1093/pcp/pcy061
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• [Journal Article] Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman (2018)
  • Author(s): Oikawa Takaya、Ishimaru Yasuhiro、Munemasa Shintaro、Takeuchi Yusuke、Washiyama Kento、Hamamoto Shin、Yoshikawa Nobuyuki、Mutara Yoshiyuki、Uozumi Nobuyuki、Ueda Minoru
  • Journal Title: Current Biology Volume: 28 Issue: 14 Pages: 2230-2238
  • DOI: 10.1016/j.cub.2018.05.042
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• [Journal Article] Identification of regions responsible for the function of the plant K+ channels KAT1 and AKT2 in Saccharomyces cerevisiae and Xenopus laevis oocytes (2017)
  • Author(s): Saito Shunya、Hoshi Naomi、Zulkifli Lalu、Widyastuti Sri、Goshima Shinobu、Dreyer Ingo、Uozumi Nobuyuki
  • Journal Title: Channels Volume: 11 Issue: 6 Pages: 510-516
  • DOI: 10.1080/19336950.2017.1372066
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Probing native metal ion association sites through quenching of fluorophores in the nucleotide-binding domains of the ABC transporter MsbA (2017)
  • Author(s): Daiki Tatsumi, Kei Nanatani, Yuto Koike, Kiyoto Kamagata, Satoshi Takahashi, Ayumu Konno, Tadaomi Furuta, Minoru Sakurai, Nobuyuki Uozumi
  • Journal Title: Biochem. J. Volume: 474 Issue: 12 Pages: 1993-2007
  • DOI: 10.1042/bcj20161051
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• [Journal Article] Kup-mediated Cs+ uptake and Kdp-driven K+ uptake coordinate to promote cell growth during excess Cs+ conditions in Escherichia coli (2017)
  • Author(s): Ellen Tanudjaja, Naomi Hoshi, Yi-Hsin Su, Shin Hamamoto & Nobuyuki Uozumi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 1-9
  • DOI: 10.1038/s41598-017-02164-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The topogenic function of S4 promotes membrane insertion of the voltage-sensor domain in the KvAP channel (2016)
  • Author(s): Eriko Mishima, Yoko Sato, Kei Nanatani, Naomi Hoshi, Jong-Kook Lee, Nina Schiller, Gunnar von Heijne, Masao Sakaguchi, Nobuyuki Uozumi
  • Journal Title: Biochem. J. Volume: 473 Pages: 4361-4372
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 藍藻の新規強光応答機構を担う Na+/H+ アンチポーターの同定と機能解析 (2021)
  • Author(s): 小林歩夢, 辻井雅, 狩野文香, 解良康太, 児島征司, 小口理一, 彦坂幸毅, 園池公毅, 魚住信之
  • Organizer: 日本農芸化学会 2021年度大会
• [Presentation] 光合成電子伝達系を調節するイオン輸送体と硫黄分子の解析 (2021)
  • Author(s): 辻井雅、石丸泰寛、魚住信之
  • Organizer: 第一回 R＆D 戦略委員会 サテライトシンポジウム
• [Presentation] 強光適応と光合成調節にかかわる藍藻 Na+/H+ アンチポーターの解析 (2020)
  • Author(s): 小林歩夢, 辻井雅, 狩野文香, 解良康太, 石丸泰寛, 魚住信之
  • Organizer: 第15回トランスポーター研究会年会
• [Presentation] 気孔閉鎖を抑制する化合物の同定 (2020)
  • Author(s): 佐藤奏音，齋藤俊也, 筧太心, 河野優, Matteo Grenz, Alex Costa, 石丸泰寛, 魚住信之
  • Organizer: 植物化学調節学会第55回大会
• [Presentation] Multiple CBL-CIPK pairs regulate Arabidopsis guard cell ion channels (2019)
  • Author(s): Shunya Saito, Misaki Sato, Shin Hamamoto, Philipp Koster, Jorg Kudla and Nobuyuki Uozum
  • Organizer: 18th International Workshop on Plant Membrane Biology
  • Int'l Joint Research
• [Presentation] Elucidation of the mechanism of Na+ and Cs+-induced cell growth through cation transporters (2019)
  • Author(s): Nobuyuki Uozumi, Masaru Tsujii, Ellen Tanudjaja and Kota Kera
  • Organizer: 18th International Workshop on Plant Membrane Biology
  • Int'l Joint Research
• [Presentation] 気孔開閉と根長の制御をめざした植物イオン輸送体の活性化剤・阻害剤の探索 (2019)
  • Author(s): 魚住信之
  • Organizer: 日本生物工学会北日本支部仙台シンポジウム
  • Invited
• [Presentation] 気孔開口を抑制するKチャネル阻害剤の同定とその構造活性相関 (2019)
  • Author(s): 鈴木喬太，遠藤 晃輔，島田 友輝，有澤 美枝子，熊田 佳菜子 ，一刀 かおり，谷井 沙織，古田 未有，井坂 修久，山口 利男，Khurram Basir，関 原明，浜本 晋，石丸 泰寛，魚住 信之
  • Organizer: 第71回日本生物工学会大会
• [Presentation] Uniquely evolved plant ion transport system (2019)
  • Author(s): Nobuyuki Uozumi
  • Organizer: The 4th Symposium on Plant Environmental Sensing
  • Int'l Joint Research / Invited
• [Presentation] 光合成活性調節に関与する藍藻 Na+/H+ アンチポーターの解析 (2019)
  • Author(s): 辻井雅、狩野文香、小林歩夢、解良康太、児島征児、小口理一、彦坂公毅、園池公毅、魚住信之
  • Organizer: 日本農芸化学会2020年度大会
  • Invited
• [Presentation] 駆動力形成を担うイオン輸送による光合成調節 (2018)
  • Author(s): 魚住信之，辻井雅，狩野文香，解良康太，浜本晋，斎藤俊也
  • Organizer: 第70回日本生物工学会大会
  • Invited
• [Presentation] Dual lipidation on Ca2+-dependent kinases governs SLAC1-mediated stomatal closure (2018)
  • Author(s): Shunya Saito, Shin Hamamoto, Nobuyuki Uozumi
  • Organizer: International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
  • Int'l Joint Research
• [Presentation] Optimum Control of Cellular Homeostasis in Photosynthetic Organism, Cyanobactria and Plant Cells (2018)
  • Author(s): Nobuyuki Uozumi
  • Organizer: プラズマフォーラム
  • Invited
• [Presentation] シアノバクテリアのバイオフィルム形成に関与するポリアミン合成酵素の解析 (2018)
  • Author(s): 解良康太、永山達也、七谷圭、佐伯-矢本千香、富永昴、相馬聡、三浦のぞみ、武田幸太、茅森俊介、安藤英司、東恭平、木花将 、五十嵐一衛、魚住信之
  • Organizer: 第60回植物生理学会年会
• [Presentation] 大腸菌を宿主細胞としたシロイヌナズナの K+/H+ アンチポーターの機能解析 (2017)
  • Author(s): 辻井雅，解良康太，浜本晋，黒森崇，鹿内利治，魚住信之
  • Organizer: 第12回トランスポーター研究会
• [Presentation] シロイヌナズナの Non-photochemical quenchingに関与するK+/H+アンチポーターの輸送活性の評価 (2017)
  • Author(s): 辻井雅, 解良康太, 浜本晋, 黒森崇，鹿内利治，魚住信之
  • Organizer: 第69回日本生物工学会大会
• [Presentation] ショ糖密度勾配遠心分離法によるN+/H+アンチポーターの細胞内局在性の解析 (2017)
  • Author(s): 狩野文香、解良康太、辻井雅、魚住信之
  • Organizer: 藍藻の分子生物学
• [Presentation] シアノバクテリアトランスポーターの局在性および機能解析 (2017)
  • Author(s): 狩野文香、辻井雅、解良康太、魚住信之
  • Organizer: 第59回日本植物生理学会年会
• [Patent(Industrial Property Rights)] ジフェニル尿素化合物誘導体 (2019)
  • Inventor(s): 魚住信之，有澤美枝子，鈴木喬太
  • Industrial Property Rights Holder: 魚住信之，有澤美枝子，鈴木喬太
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] 植物のカリウムイオン輸送体の機能制御剤及び植物の育成方法 (2019)
  • Inventor(s): 魚住信之，有澤美枝子，鈴木喬太，遠藤晃輔，浜本晋，池ノ上芳章
  • Industrial Property Rights Holder: 魚住信之，有澤美枝子，鈴木喬太，遠藤晃輔，浜本晋，池ノ上芳章
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] 植物のカリウムイオン輸送体の機能制御剤及び植物の育成方法 (2018)
  • Inventor(s): 魚住信之 遠藤晃輔 浜本晋 鈴木喬太 池ノ上芳章
  • Industrial Property Rights Holder: 魚住信之 遠藤晃輔 浜本晋 鈴木喬太 池ノ上芳章
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-020806
  • Filing Date: 2018
• [Patent(Industrial Property Rights)] 植物のカリウムイオン輸送体の機能制御剤及び植物の育成方法 (2017)
  • Inventor(s): 魚住信之 遠藤晃輔 浜本晋 池ノ上芳章
  • Industrial Property Rights Holder: 魚住信之 遠藤晃輔 浜本晋 池ノ上芳章
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-042163
  • Filing Date: 2017