| Budget Amount *help |
¥104,650,000 (Direct Cost: ¥80,500,000、Indirect Cost: ¥24,150,000)
Fiscal Year 2021: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2020: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2019: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2018: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2017: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
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| Outline of Final Research Achievements |
In order to investigate the effect of intracellular multimolecular crowding environment on biological functions, we developed devices to construct and control artificial multimolecular crowding environment. First, we prepared a device in which E. coli was fused into a microchamber to control the intracellular multimolecular crowding environment, and examined its transcriptional and translational activities against the degree of cytoplasmic dilution, but no correlation was found between them. An artificial multimolecular crowding environment was subsequently prepared by osmolyte molecules and correlated with the activity of the in vitro transcription-translation system, and the effect was observed for several proteins. We also developed a system that enables continuous solution exchange in a microchamber device, and succeeded in developing a system that changes osmolarity by changing the solution in and out of the chamber.
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