Biomodulation by calpain

• Project Area: Proteolysis in the Regulation of Biological Processes
• Project/Area Number: 18076007
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: 財団法人東京都医学研究機構 (2010); Tokyo Metropolitan Organization for Medical Research (2006-2009)
• Principal Investigator: SORIMACHI Hiroyuki 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 参事研究員 (10211327)
• Co-Investigator(Kenkyū-buntansha): ONO Yasuko 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 主席研究員 (20392376) ; HATA Shoji 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 主席研究員 (10392375) ; OJIMA Koichi 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所・食肉プロテオーム研究チーム, 研究員 (60415544)
• Project Period (FY): 2006 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥83,400,000 (Direct Cost: ¥83,400,000); Fiscal Year 2010: ¥23,000,000 (Direct Cost: ¥23,000,000); Fiscal Year 2009: ¥26,200,000 (Direct Cost: ¥26,200,000); Fiscal Year 2008: ¥25,500,000 (Direct Cost: ¥25,500,000); Fiscal Year 2007: ¥4,500,000 (Direct Cost: ¥4,500,000); Fiscal Year 2006: ¥4,200,000 (Direct Cost: ¥4,200,000)
• Keywords: 細胞内タンパク質分解 / カルパイン / カルシウム / 骨格筋 / 胃腸 / 酵素 / 蛋白質 / プロテオリシス / メンブレン・トラフィック / モジュレータ・プロテアーゼ / 筋ジストロフィー / ノックインマウス / ユビキチンリガーゼ / シグナル伝達

Research Abstract

"Calpains", which are encoded by 15 genes in humans, constitute a family of intracellular Ca^<2+>-dependent "modulator" proteases. They function to maintain homeostasis of various living organisms, and, therefore, defects in calpain functions result in lethality or diseases like muscular dystrophy and stress-induced stomach ulcer. In this study, we focused on tissue-specific calpains, which is involved in tissue-specific functions where they are expressed. As a result, we could elucidate at least a part of molecular mechanisms how defects of calpains cause various disorders.

Research Products

• [Journal Article] Chapter 453-6 and 458. μ-calpain, m-calpain, Muscle calpain, Gastrointestinal calpain, and other calpains. (2012)
  • Author(s): Sorimachi H, Ono Y, Hata S
  • Journal Title: Handbook of Proteolytic enzymes, 3rd Edition(Rawlings ND, Salvesen G, eds)(Oxford, UK: Elsevier Academic Press.) Volume: in press
  • URL: http://store.elsevier.com/product.jsp?Isbn=9780123822192&pagename=search
  • Peer Reviewed
• [Journal Article] Calpain (2012)
  • Author(s): Sorimachi H
  • Journal Title: Encyclopedia of Biological Chemistry 2nd Edition(Lane MD, Lennarz WJ, eds., Oxford, UK: Elsevier Academic Press) Volume: in press
  • URL: http://store.elsevier.com/product.jsp?isbn=9780080547756&pagename=search
  • Peer Reviewed
• [Journal Article] Calpains - An elaborate proteolytic system (2012)
  • Author(s): Ono Y, Sorimachi H
  • Journal Title: Biochim Biophys Acta Volume: 1824 Issue: 1 Pages: 224-236
  • DOI: 10.1016/j.bbapap.2011.08.005
  • Peer Reviewed
• [Journal Article] Efficient expression and purification of recombinant human m-calpain using an Escherichia coli expression system at low temperature. (2012)
  • Author(s): Hata S, Ueno M, Kitamura F, Sorimachi H
  • Journal Title: J Biochem Volume: 151 Issue: 4 Pages: 417-422
  • DOI: 10.1093/jb/mvs002
  • NAID: 10031166080
  • Peer Reviewed
• [Journal Article] Functional Evolution of Duplicated Odorant-Binding Protein Genes, Obp57d and Obp57e, in Drosophila (2012)
  • Author(s): Eriko Harada
  • Journal Title: PLoS ONE Volume: 7(1) Issue: 1 Pages: e29710-e29710
  • DOI: 10.1371/journal.pone.0029710
  • Peer Reviewed
• [Journal Article] Calpain chronicle--an enzyme family under multidisciplinary characterization. (2011)
  • Author(s): Sorimachi H, HataS, Ono Y
  • Journal Title: Proc Jpn Acad Ser B Phys Biol Sci Volume: 87 Pages: 287-327
  • NAID: 130000903736
  • Peer Reviewed
• [Journal Article] Calpain cleavage prediction using multiple kernel learning. (2011)
  • Author(s): duVerle DA, Ono Y, Sorimachi H, Mamitsuka H
  • Journal Title: PLoS One Volume: 6 Issue: 5 Pages: e19035-e19035
  • DOI: 10.1371/journal.pone.0019035
  • Peer Reviewed
• [Journal Article] Does the severity of the LGMD2A phenotype in compound heterozygotes depend on the combination of mutations? (2011)
  • Author(s): Saenz A, Ono Y, Sorimachi H, Goicoechea M, Leturcq F, Blazquez L, Garcia-Bragado F, Marina A, Poza JJ, Azpitarte M, Doi N, Urtasun M, Kaplan JC, Lopez de Munain A
  • Journal Title: Muscle and Nerve Volume: 44 Issue: 5 Pages: 710-714
  • DOI: 10.1002/mus.22194
  • Peer Reviewed
• [Journal Article] Non-proteolytic functions of calpain-3 in sarcoplasmic reticulum in skeletal muscles. (2011)
  • Author(s): Ojima K, Ono Y, Ottenheijm C, HataS, Suzuki H, Granzier H, Sorimachi H
  • Journal Title: J Mol Biol Volume: 407 Issue: 3 Pages: 439-449
  • DOI: 10.1016/j.jmb.2011.01.057
  • Peer Reviewed
• [Journal Article] Impact of genetic insights into calpain biology (2011)
  • Author(s): Sorimachi H, Hata S, Ono Y
  • Journal Title: J Biochem Volume: 150 Issue: 1 Pages: 23-37
  • DOI: 10.1093/jb/mvr070
  • NAID: 10030586192
  • Peer Reviewed
• [Journal Article] Calpain-6, a microtubule-stabilizing protein, regulates Rac1 activity and cell motility through interaction with GEF-H1 (2011)
  • Author(s): Kazuo Tonami
  • Journal Title: Journal of Cell Science Volume: 124巻 Issue: 8 Pages: 1214-1223
  • DOI: 10.1242/jcs.072561
  • Peer Reviewed
• [Journal Article] Dynamic distribution of muscle-specific calpain in mice has a key role in physical-stress adaptation and is impaired in muscular dystrophy. (2010)
  • Author(s): Ojima K, Kawabata Y, Nakao H, Nakao K, Doi N, Kitamura F, Ono Y, HataS, Suzuki H, Kawahara H, Bogomolovas J,Will C, Ottenheijm C, LabeitS, Granzier H, Toyama-Sorimachi N, Sorimachi M, Suzuki K, Maeda T, Abe K, Aiba A, Sorimachi H
  • Journal Title: J Clin Invest Volume: 120 Issue: 8 Pages: 2672-2683
  • DOI: 10.1172/jci40658
  • Peer Reviewed
• [Journal Article] Skeletal muscle-specific calpain, p94/calpain-3, dynamically distributes in skeletal muscle cells to adapt to physical stress, defects of which cause muscular dystrophy. (2010)
  • Author(s): Ojima K, Ono Y, Doi N, Kitamura F, Kawabata Y, Suzuki K, Maeda T, Abe K, Nakao H, Aiba A, Suzuki H, Kawahara H, Witt C,Ottenheijm C, Labeit S, Ottenheijm C, Granzier H, Toyama-Sorimachi N, Sorimachi M, Sorimachi H
  • Journal Title: Neuromuscul Disord Volume: 20 Pages: 598-599
  • URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/973/description
  • Peer Reviewed
• [Journal Article] Redundant and non-redundant effects of Ca^<2+> and Na^+ on the activation of p94/calpain-3. (2010)
  • Author(s): Ono Y, Ojima K, Torii F, Takaya E, Doi N, Nakagawa K, Hata S, Abe K, Sorimachi H
  • Journal Title: Neuromuscul Disord Volume: 20 Pages: 608-608
  • URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/973/description
  • Peer Reviewed
• [Journal Article] Expanding members and roles of the calpain superfamily and their genetically modified animals. (2010)
  • Author(s): Sorimachi H, Hata S, Ono Y
  • Journal Title: Exp Anim Volume: 59 Pages: 549-566
  • NAID: 130000419250
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21030783
  • Peer Reviewed
• [Journal Article] Une contribution a l'histoire de la decouverte des calpainopathies.(in French). (2010)
  • Author(s): Fardeau M, Beckmann JS, Sorimachi H, Urtizberea JA
  • Journal Title: Les Cahiers de Myologie Volume: 2 Pages: 5-10
• [Journal Article] CaMPDB: a resource for calpain and modulatory proteolysis. (2010)
  • Author(s): duVerle DA, Takigawa I, Ono Y, Sorimachi H, Mamitsuka H
  • Journal Title: Genome Inform Volume: 22 Pages: 202-213
  • Peer Reviewed
• [Journal Article] Skeletal muscle-specific calpain is an intracellular Na^+-dependent protease. (2010)
  • Author(s): Ono Y, Ojima K, Torii F, Takaya E, Doi N, Nakagawa K, Hata S, Abe K, Sorimachi H
  • Journal Title: J Biol Chem Volume: 285 Issue: 30 Pages: 22986-22998
  • DOI: 10.1074/jbc.m110.126946
  • Peer Reviewed
• [Journal Article] Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense. (2010)
  • Author(s): Hata S, Abe M, Suzuki H, Kitamura F, Toyama-Sorimachi N, Abe K, Sakimura K, Sorimachi H
  • Journal Title: PLoS Genet Volume: 6 Issue: 7 Pages: e1001040-e1001040
  • DOI: 10.1371/journal.pgen.1001040
  • Peer Reviewed
• [Journal Article] Dynamic distribution of muscle-specific calpain in mice has a key role in physical-stress adaptation and is impaired in muscular dystrophy (2010)
  • Author(s): Ojima, K., Kawabata, Y., Nakao, H., Nakao, K., Doi, N., Kitamura, F., Ono, Y., Hata, S., Suzuki, H., Kawahara, H., Bogomolovas, J., Witt, C., Ottenheijm, C., Labeit, S., Granzier, H., Toyama-Sorimachi, N., Sorimachi, M., Suzuki, K., Maeda, T., Abe, K., Aiba, A., Sorimachi, H.
  • Journal Title: J.Clin.Invest. Volume: 120 Pages: 2672-2683
  • Peer Reviewed
• [Journal Article] Calpain 8/nCL-2 and Calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense (2010)
  • Author(s): Hata, S., Abe, M., Suzuki, H., Kitamura, F., Toyama-Sorimachi, N., Abe, K., Sakimura, K., Sorimachi, H.
  • Journal Title: PLoS Genet. Volume: 6
  • Peer Reviewed
• [Journal Article] Skeletal muscle-specific calpain is an intracellular Na^+-dependent protease (2010)
  • Author(s): Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., Sorimachi, H.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 22986-22998
  • Peer Reviewed
• [Journal Article] Inhibition of calpain increases LIS1 expression and partially rescues in vivo phenotypes in a mouse model of lissencephaly. (2009)
  • Author(s): Yamada M, Yoshida Y, Mori D, Takitoh T, Kengaku M, Umeshima H, Takao K, Miyakawa T, Sato M, Sorimachi H, Wynshaw-Boris A, Hirotsune S
  • Journal Title: Nat Med Volume: 15 Issue: 10 Pages: 1202-1207
  • DOI: 10.1038/nm.2023
  • Peer Reviewed
• [Journal Article] CaMPDB : a Resource for Calpain and Modulatory Proteolysis. (2009)
  • Author(s): duVerle, D.A., Takigawa, I., Ono, Y., Sorimachi, H., Mamitsuka, H.
  • Journal Title: Genome Informatics 22 Pages: 202-214
  • Peer Reviewed
• [Journal Article] Inhibition of calpain increases LIS1(PAFAH1B1) and partially rescues in vivo phenotypes in a mouse model of lissencephaly. (2009)
  • Author(s): Yamada, M., Yoshida, Y., Mori, D., Takitoh, T., Kengaku, M., Umeshima, H., Takao, K., Miyakawa, T., Sato, M., Sorimachi, H., Wynshaw-Boris, A., Hirotsune, S.
  • Journal Title: Nat.Med. 15 Pages: 1202-1207
  • Peer Reviewed
• [Journal Article] Muscle RING-finger protein-1 (MuRF1) as a connector of muscle energy metabolism and protein synthesis. (2008)
  • Author(s): Koyama S, HataS, Witt CC, Ono Y, Lerche S, Ojima K, Chiba T, Doi N, Kitamura F, Tanaka K, Abe K, Witt SH, Rybin V, Gasch A, Franz T, Labeit S, Sorimachi H
  • Journal Title: J Mol Biol Volume: 376 Issue: 5 Pages: 1224-1236
  • DOI: 10.1016/j.jmb.2007.11.049
  • Peer Reviewed
• [Journal Article] Single-cell chemical lysis method for analyses of intracellular molecules using an array of picoliter-scale microwells. (2008)
  • Author(s): Sasuga Y, Iwasawa T, Terada K, Oe Y, Sorimachi H, Ohara O, Harada Y
  • Journal Title: Anal Chem Volume: 80 Issue: 23 Pages: 9141-9149
  • DOI: 10.1021/ac8016423
  • Peer Reviewed
• [Journal Article] The importance of conserved amino acid residues in p94 protease sub-domain IIb and the IS2 region for constitutive autolysis. (2008)
  • Author(s): Ono Y, Hayashi C, Doi N, Tagami M, Sorimachi H
  • Journal Title: FEBS Lett Volume: 582 Pages: 691-698
  • Peer Reviewed
• [Journal Article] Multiple molecular interactions implicate the connectin/titin N2A region as a modulating scaffold for p94/calpain 3 activity in skeletal muscle. (2008)
  • Author(s): Hayashi C, Ono Y, Doi N, Kitamura F, Tagami M, Mineki R, Arai T, Taguchi H, Yanagida M, Hirner S, Labeit D, Labeit S, Sorimachi H
  • Journal Title: J Biol Chem Volume: 283 Pages: 14801-14
  • Peer Reviewed
• [Journal Article] Multiple molecular interactions implicate connectin/titin N2A region as a modulating scaffold for p94/calpain 3 activityin skeletal muscle (2008)
  • Author(s): Hayashi, C., Ono, Y., Doi, N., Kitamura, F., Tagami, M., Mineki, R., Arai, T., Taguchi, H., Yanagida, M., Hirner, S., Labeit, D., Labeit, S., Sorimachi, H
  • Journal Title: J. Biol. Chem 283 Pages: 14801-14814
  • Peer Reviewed
• [Journal Article] The importance of conserved amino acid residues in protease sub-domain IIb and the IS2 region in p94 for constitutive autolysis (2008)
  • Author(s): Ono, Y., Hayashi, C., Doi, N., Tagami, M., Sorimachi, H
  • Journal Title: FEBS Lett 582 Pages: 691-698
  • Peer Reviewed
• [Journal Article] Muscle RING-finger protein MuRF1 as a connector of muscle energy metabolism and protein synthesis (2008)
  • Author(s): Koyama, S., Hata, S., Witt, C. C. , Ono, Y., Lerche, S., Ojima, K., Chiba, T., Doi, N., Kitamura, F., Tanaka, K., Abe, K., Witt, S., Rybin, V., Gasch, A., Franz, T., Labeit, S., Sorimachi, H
  • Journal Title: J. Mol. Biol 376 Pages: 1224-1236
  • Peer Reviewed
• [Journal Article] Multiple molecular interactions implicate connectin/titin N2A region as a modulating scaffold for p94/caplain 3 activity in skeletal muscle. (2008)
  • Author(s): Hayashi, C., Ono, Y., Doi, N., Kitamura, F., Tagami, M., Mineki, R., Arai, T., Taguchi, H., Yanagida, M., Hirner, S., Labeit, D., Labeit, S., and Sorimachi, H.
  • Journal Title: J. Biol. Chem. 283 Pages: 14801-14814
  • Peer Reviewed
• [Journal Article] A simple single-cell chemical lysis method for analyses of intracellular molecules using an array of picoliter-scale microwells. (2008)
  • Author(s): Sasuga, Y., Iwasawa, T., Terada, K., Oe, Y., Sorimachi, H., Ohara, O., and Harada, Y.
  • Journal Title: Anal. Chem. 80 Pages: 9141-9149
  • Peer Reviewed
• [Journal Article] The importance of conserved amino acid residues in protease sub-domain Iib and the IS2 region in p94 for constitutive autolysis. (2008)
  • Author(s): Ono, Y., Hayashi, C., Doi, N., Tagami, M., and Sorimachi, H.
  • Journal Title: FEBS Lett. 582 Pages: 691-698
  • Peer Reviewed
• [Journal Article] Multiple molecular interactions implicate connectin/titin N2A region as a modulating scaffold for p94/calpain 3 activity in skeletal muscle. (2008)
  • Author(s): Hayashi, C
  • Journal Title: J.Biol.Chem. 283 Pages: 14801-14814
  • Peer Reviewed
• [Journal Article] Muscle RING-Finger Protein MuRFl as a Connector of Muscle Energy Metabolism and Protein Synthesis. (2008)
  • Author(s): Koyama, S
  • Journal Title: J.Mol.Biol. 376 Pages: 1224-1236
  • Peer Reviewed
• [Journal Article] Comprehensive survey of p94/calpain 3 substrates by comparative proteomics--possible regulation of protein synthesis by p94. (2007)
  • Author(s): Ono Y, Hayashi C, Doi N, Kitamura F, Shindo M, Kudo K, Tsubata T, Yanagida M, Sorimachi H
  • Journal Title: Biotech J Volume: 2 Issue: 5 Pages: 565-576
  • DOI: 10.1002/biot.200700018
  • Peer Reviewed
• [Journal Article] Myogenic stage, sarcomere length, and protease activity modulate localization of muscle-specific calpain. (2007)
  • Author(s): Ojima K, Ono Y, Doi N, Yoshioka K, Kawabata Y, Labeit S, Sorimachi H
  • Journal Title: J Biol Chem Volume: 282 Issue: 19 Pages: 14493-14504
  • DOI: 10.1074/jbc.m610806200
  • Peer Reviewed
• [Journal Article] Suppression of calpain-dependent cleavage of the CDK5 activator p35 to p25 by site-specific phosphorylation. (2007)
  • Author(s): Kamei H, Saito T, Ozawa M, Fujita Y, Asada A, Bibb JA, Saido TC, Sorimachi H, Hisanaga S
  • Journal Title: J Biol Chem Volume: 282 Issue: 3 Pages: 1687-1694
  • DOI: 10.1074/jbc.m610541200
  • Peer Reviewed
• [Journal Article] Stomach-specific calpain, nCL-2/calpain 8, is active without calpain regulatory subunit and oligomerizes through C2-like domains. (2007)
  • Author(s): Hata S, Doi N, Kitamura F, Sorimachi H
  • Journal Title: J Biol Chem Volume: 282 Issue: 38 Pages: 27847-56
  • DOI: 10.1074/jbc.m703168200
  • Peer Reviewed
• [Journal Article] Stomach-specific calpain, nCL-2/calpain 8, is activewithout calpain regulatory subunit, and oligomerizes through C2-like domains (2007)
  • Author(s): Hata, S., Doi, N., Kitamura, F., Sorimachi, H
  • Journal Title: J. Biol. Chem 282 Pages: 27847-27856
  • Peer Reviewed
• [Journal Article] Myogenic stage, sarcomere length and protease activity modulate localization ofmuscle-specific calpain (2007)
  • Author(s): Ojima, K., Ono, Y., Doi, N., Yoshioka, K., Kawabata, Y., Labeit, S., Sorimachi, H
  • Journal Title: J. Biol. Chem 282 Pages: 14493-14504
  • Peer Reviewed
• [Journal Article] Comprehensive survey of p94/calpain 3 substrates by comparative proteomics - Possible regulation of protein synthesis by p94 (2007)
  • Author(s): Ono, Y., Hayashi, C., Doi, N., Kitamura, F., Shindo, M., Kudo, K., Tsubata, T., Yanagida, M., Sorimachi, H
  • Journal Title: Biotech. J 2 Pages: 565-576
  • Peer Reviewed
• [Journal Article] Stomach-specific calpain,nCL-2/calpain 8,is active without calpain regulatory subunit, and oligomerizes through C2-like domains (2007)
  • Author(s): Hata, S
  • Journal Title: J.Biol.Chem. 282 Pages: 27847-27856
  • Peer Reviewed
• [Journal Article] Suppression of calpain-dependent cleavage of the Cdk5 activator p35 to p25 by site-specific phosphorylation. (2007)
  • Author(s): Kamei, H. et al.
  • Journal Title: J. Biol. Chem. 282(in press)
• [Journal Article] Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the β-subunit of coatomer complex, β-COP. (2006)
  • Author(s): Hata S, Koyama S, Kawahara H, Doi N, Maeda T, Toyama-Sorimachi N, Abe K, Suzuki K, Sorimachi H
  • Journal Title: J Biol Chem Volume: 281 Issue: 16 Pages: 11214-24
  • DOI: 10.1074/jbc.m509244200
  • NAID: 80019185214
  • Peer Reviewed
• [Journal Article] Extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by Aspergillus oryzae. (2006)
  • Author(s): Nakajima K, Asakura T, Maruyama J, Morita Y, Oike H, Shimizu-Ibuka A, Misaka T, Sorimachi H, Arai S, Kitamoto K, Abe K
  • Journal Title: Appl Environ Microbiol Volume: 72 Issue: 5 Pages: 3716-3723
  • DOI: 10.1128/aem.72.5.3716-3723.2006
  • Peer Reviewed
• [Journal Article] Neoculin, a taste-modifying protein, is recognized by human sweet taste receptor. (2006)
  • Author(s): Nakajima K, Asakura T, Oike H, Morita Y, Shimizu-Ibuka A, Misaka T, Sorimachi H, Arai S, Abe K
  • Journal Title: Neuroreport Volume: 17 Issue: 12 Pages: 1241-44
  • DOI: 10.1097/01.wnr.0000230513.01339.3b
  • Peer Reviewed
• [Journal Article] Spatio-temporal expression of muscle-specific calpain, p94/calpain 3, during myogenesis. (2006)
  • Author(s): Ojima K, Ono Y, Doi N, Labeit S, Sorimachi H
  • Journal Title: Neuromuscul Disord Volume: 16
  • URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/973/description.
  • Peer Reviewed
• [Journal Article] Calpain system regulates the differentiation of adult primitive mesenchymal ST-13 adipocytes. (2006)
  • Author(s): Yajima Y, Sato M, Sorimachi H, Inomata M, Maki M, Kawashima S
  • Journal Title: Endocrinology Volume: 147 Issue: 10 Pages: 4811-4819
  • DOI: 10.1210/en.2005-1647
  • Peer Reviewed
• [Journal Article] Suppressed disassembly of autolyzing p94/CAPN3 by N2A connectin/titin in a genetic reporter system. (2006)
  • Author(s): Ono Y, Torii F, Ojima K, Doi N, Yoshioka K, Kawabata Y, Labeit D, LabeitS, Suzuki K, Abe K, Maeda T, Sorimachi H
  • Journal Title: J Biol Chem Volume: 281 Issue: 27 Pages: 18519-31
  • DOI: 10.1074/jbc.m601029200
  • Peer Reviewed
• [Journal Article] Nutrient-dependent multimerization of the mammalian target of rapamycin through the N-terminal HEAT repeat region. (2006)
  • Author(s): Takahara T, Hara K, Yonezawa K, Sorimachi H, Maeda T
  • Journal Title: J Biol Chem Volume: 281 Pages: 28605-28614
  • Peer Reviewed
• [Journal Article] How do defects in proteolytic functions of skeletal muscle-specific calpain, p94/calpain 3, cause calpainopathy/LGMD2A? (2006)
  • Author(s): Sorimachi H, Ojima K, Yanagida M, Ogawa H, Hayashi C, Doi N, Ono Y
  • Journal Title: Neuromuscul Disord Volume: 16
  • URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/973/description.
  • Peer Reviewed
• [Journal Article] Crystal structure of neoculin: insights into its sweetness and taste-modifying activity. (2006)
  • Author(s): Shimizu-Ibuka A, Morita Y, Terada T, Asakura T, Nakajima K, Iwata S, Misaka T, Sorimachi H, Arai S, Abe K
  • Journal Title: J Mol Biol Volume: 359 Issue: 1 Pages: 148-158
  • DOI: 10.1016/j.jmb.2006.03.030
  • Peer Reviewed
• [Journal Article] Effect of N2A connectin/titin on disassembly of p94/CAPN3 caused by auaolysis in IS2. (2006)
  • Author(s): Ono Y, Torii F, Ojima K, Doi N, Yoshioka K, Labeit D, Labeit S, Suzuki K, Abe K, Maeda T, Sorimachi H
  • Journal Title: FEBS Journal Volume: 273 Supp1. Issue: s1 Pages: 50-50
  • DOI: 10.1111/j.1742-4658.2006.05276.x
  • Peer Reviewed
• [Journal Article] Relationships between p94/CAPN3 and N2A connectin/titin in skeletal muscle under the normal and dystrophic conditions. (2006)
  • Author(s): Ono Y, Ojima K, Doi N, Hayashi C, Torii F, 40K, Labeit D, Labeit S, Suzuki K, Abe K, Maeda T, Sorimachi H
  • Journal Title: Neuromuscul Disord Volume: 16
  • URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/973/description.
  • Peer Reviewed
• [Journal Article] Suppressed disassembly of autolyzing p94/CAPN3 in a genetic reporter system (2006)
  • Author(s): Ono, Y., Torii, F., Ojima, K., Doi, N., Yoshioka, K., Kawabata, Y., Labeit, D., Labeit, S., Suzuki,K., Abe, K., Maeda, T., Sorimachi, H
  • Journal Title: J. Biol. Chem 281 Pages: 18519-18531
  • Peer Reviewed
• [Journal Article] Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the β-subunitof coatomer complex (2006)
  • Author(s): Hata, S., Koyama, S., Kawahara, H., Doi, N., Maeda, T., Toyama-Sorimachi, N., Abe, K., Suzuki, K., Sorimachi, H
  • Journal Title: β-COP. J. Biol. Chem 281 Pages: 11214-11224
  • Peer Reviewed
• [Journal Article] Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the β-subunit of coatomer complex, β-COP. (2006)
  • Author(s): Hata, S. et al.
  • Journal Title: J. Biol. Chem. 281 Pages: 11214-11224
• [Journal Article] N2A connectin/titin suppressed disassembly of p94/CAPN3 caused by autolysis of IS2 but not IS1 in a genetic reporter system. (2006)
  • Author(s): Ono, Y.et al.
  • Journal Title: J. Biol. Chem. 281 Pages: 18519-18531
• [Journal Article] Nutrient-dependent multimerization of the mammalian target of rapamycin through the N-terminal HEAT repeat region. (2006)
  • Author(s): Takahara, T. et al.
  • Journal Title: J. Biol. Chem. 281 Pages: 28605-14
• [Journal Article] Calpain system regulates the differentiation of adult primitive mesenchymal ST-13. (2006)
  • Author(s): Yajima, Y. et al.
  • Journal Title: Endocrinology 147 Pages: 4811-4819
• [Journal Article] Crystal structure of neoculin : insights into its sweetness and taste-modifying activity. (2006)
  • Author(s): Shimizu-Ibuka, A et al.
  • Journal Title: J. Mol. Biol. 359 Pages: 148-158
• [Presentation] Studies of titin signaling and CAPN3. (2011)
  • Author(s): Sorimachi, H., Ojima, K., and Ono, Y.
  • Organizer: LGMD2A Workshop: Coalition to Cure Calpain 3(C3)
  • Place of Presentation: Santa Monica, USA.
  • Year and Date: 2011-10-27
• [Presentation] Unique properties of muscle-specific calpain and muscular dystrophy caused by its defect. (2011)
  • Author(s): Sorimachi, H., Ojima, K., and Ono, Y.
  • Organizer: IPS2011 - Seventh General Meeting of the International Proteolysis Society
  • Place of Presentation: San Diego, USA.
• [Presentation] Skeletal muscle-specific calpain is an intracellular Ca^<2+> and Na^+-dependent protease. (2011)
  • Author(s): Sorimachi, H., Ojima, K., Takaya, E., and Ono Y.
  • Organizer: CaBP17 - The 17th International Symposium on Ca^<2+>-binding proteins and Ca^<2+> function in health and disease.
  • Place of Presentation: Beijing, China.
• [Presentation] Calpains-introduction (2010)
  • Author(s): Ono, Y., Hata, S., and Sorimachi, H.
  • Organizer: CHUV(Le Centre Hospitalier Universitaire Vaudois) Special Seminar
  • Place of Presentation: Lausanne, Swizerland.
  • Year and Date: 2010-10-01
• [Presentation] Unique functions of mammalian unconventional calpains (2010)
  • Author(s): Sorimachi, H.
  • Organizer: FASEB Summer Research Conferences-The Biology of Calpains in Health & Disease
  • Place of Presentation: Carefree, Arizona, USA(招待講演)
  • Year and Date: 2010-07-30
• [Presentation] The closing lecture]In Memoriam Koichi Suzuki: Calpains in muscles and stomach XIIth Symposium on Proteases (2010)
  • Author(s): Ono, Y., Hata, S., Ojima, K., Tonami, K., Kitamura, F., Ueno, M., Takaya, E., Granzier, H., Labeit, S., and Sorimachi, H.
  • Organizer: Inhibitors and Biological Control
  • Place of Presentation: Portoroz, Slovenia.
• [Presentation] (Murachi Award-winning oral presentation)Possible interacting proteins for G-calpain, a novel gastrointestinal calpain complex of calpain 8/nCL-2 and calpain 9/nCL-4. (2010)
  • Author(s): Matsushita, G, Hata, S., and Sorimachi, H.
  • Organizer: FASEB Summer Research Conferences - The Biology of Calpains in Health & Disease
  • Place of Presentation: Arizona, USA.
• [Presentation] Unique functions of mammalian unconventional calpains. (2010)
  • Author(s): Sorimachi, H., Hata, S., Ojima, K., Labeit, S., and Ono, Y.
  • Organizer: FASEB Summer Research Conferences - The Biology of Calpains in Health & Disease
  • Place of Presentation: Arizona, USA.
• [Presentation] Legacy: Structure-function relationships of calpain. (2010)
  • Author(s): Sorimachi, H., A Koichi Suzuki
  • Organizer: FASEB Summer Research Conferences - The Biology of Calpains in Health & Disease
  • Place of Presentation: Arizona, USA.
• [Presentation] (Selected for oral presentation) Calpain 8/nCL-2 and Calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense (2010)
  • Author(s): Hata, S., Abe, M., Suzuki, H., Kitamura, F., Toyama-Sorimachi, N., Abe, K., Sakimura, K., and Sorimachi, H.
  • Organizer: FASEB Summer Research Conferences - The Biology of Calpains in Health & Disease
  • Place of Presentation: Arizona, USA.
• [Presentation] (Selected for oral presentation) Characteristics of p94/calpain 3 as an intracellular Na^+-dependent protease (2010)
  • Author(s): Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., and Sorimachi, H.
  • Organizer: FASEB Summer Research Conferences - The Biology of Calpains in Health & Disease
  • Place of Presentation: Arizona, USA.
• [Presentation] Calpaines et Calpainopathies. (2009)
  • Author(s): Sorimachi, H.
  • Organizer: VIIes Journees annuelles de la SFM (Societe Francaise de Myologie)
  • Place of Presentation: Hendaye,France
  • Year and Date: 2009-10-30
• [Presentation] Calpain-3 and molecular interactions. Calpaines et Calpainopathies. (2009)
  • Author(s): Sorimachi, H., Ojima, K., Hata, S., andOno, Y.
  • Organizer: VIIes Journees annuelles de la SFM(Societe Francaise de Myologie)
  • Place of Presentation: Hendaye, France.
• [Presentation] Roles of skeletal muscle-specific calpain, p94/calpain 3, on multiple molecular interactions using connectin/titin N2A region as a modulating scaffold (2008)
  • Author(s): Sorimachi, H
  • Organizer: 11th International Symposium on PROTEINASE INHIBITORS AND BIOLOGICAL CONTROL
  • Place of Presentation: Portoroz, Slovenia
  • Year and Date: 2008-09-02
• [Presentation] La calpaina como "proteina moduladora" en la enfermedad y la salud. (Calpain as a "modulator protease" in health and muscle disease.) (2008)
  • Author(s): Sorimachi, H
  • Organizer: UPV/EHU (Universidad del Pais Vasco/Euskal Herriko Unibertsitatea) Summer Course Symposium: Distrofias por Deficit de Calpaina-3: Una Vision General
  • Place of Presentation: San Sebastian, Spain
  • Year and Date: 2008-07-14
• [Presentation] Analysis of structure-function relationships of p94 by proteinase-trapping system (2008)
  • Author(s): Ono, Y
  • Organizer: The Joint 4th AOHUP (Asian Oceania Human Proteome Organisation) and 2nd PRICPS (Pacific Rim International Conference on Protein Science)
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2008-06-22
• [Presentation] カルパイン及びコネクチンの構造-病態生理相関(Structure-Pathophysiological-Function Relationships of Calpain and Connectin/Titin.) (2008)
  • Author(s): 反町洋之、小野弥子、尾嶋孝一、林智佳子、土井奈穂子、北村ふじ子、小山傑、楠畑かおり、秦勝志、Labeit, S.
  • Organizer: 第8回日本蛋白質科学会年会 シンポジウム「蛋白質の輪廻転生-セントラルドグマを超えて」
  • Place of Presentation: 東京
  • Year and Date: 2008-06-12
• [Presentation] Roles of skeletal muscle-specific calpain, p94/calpain 3, on multiple molecular interactions using connectin/titin N2A region as a modulating scaffold. (2008)
  • Author(s): Sorimachi, H., Hayashi, C., Hata, S., Ojima, K., Koyama, S., Doi, N., Kitamura, F., Takaya, E., Ueno, M., Kaneko, N., and Ono, Y.
  • Organizer: 11th International Symposium on PROTEINASE INHIBITORS AND BIOLOGICAL CONTROL
  • Place of Presentation: Portoroz, Slovenia.
• [Presentation] Lacalpaina como "proteina moduladora" en la enfermedad y la salud.(Calpain as a "modulator protease" in health and muscle disease.) (2008)
  • Author(s): Sorimachi, H.
  • Organizer: UPV/EHU(Universidad del Pais Vasco/Euskal Herriko Unibertsitatea) Summer Course Symposium: Distrofias por Deficit de Calpaina-3: Una Vision General
  • Place of Presentation: San Sebastian, Spain.
• [Presentation] Toward Understanding Relationships between Proteolytic Activity of Skeletal Muscle-Specific Calpain and Calpainopathy. (2007)
  • Author(s): Ono, Y., Ojima, K., Hata, S., and Sorimachi, H.
  • Organizer: 2007 Korea-Japan Joint Symposium "Protein Modification and Degradation in Human Diseases"
  • Place of Presentation: Seoul, South Korea.
  • Year and Date: 2007-09-05
• [Presentation] Toward Understanding Relationships between Proteolytic Activity of Skeletal Muscle-Specific Calpain and Calpainopathy (2007)
  • Author(s): Sorimachi, H
  • Organizer: 2007 Korea-Japan Joint Symposium "Protein Modification and Degradation in Human Diseases"
  • Place of Presentation: Seoul, South Korea
  • Year and Date: 2007-09-05
• [Presentation] Unconventional calpains. FASEB Summer Research Conferences (2007)
  • Author(s): Sorimachi, H
  • Organizer: The Biology of Calpains in Health & Disease
  • Place of Presentation: Colorado, USA
  • Year and Date: 2007-07-15
• [Presentation] Unconventional calpains (2007)
  • Author(s): Sorimachi, H.
  • Organizer: FASEB Summer Research Conferences-The Biologyof Calpainsin Health & Disease
  • Place of Presentation: Colorado,USA
  • Year and Date: 2007-07-15
• [Presentation] Novel approaches for analysis of proteolytic activity of muscle-specific calpain p94/calpain 3 (2007)
  • Author(s): Sorimachi, H
  • Organizer: Xth International Symposium on PROTEINASE INHIBITORS AND BIOLOGICAL CONTROL: From single molecules to degradomics
  • Place of Presentation: Portoroz, Slovenia
  • Year and Date: 2007-06-24
• [Presentation] Unconventional calpains. (2007)
  • Author(s): Ono, Y., Ojima, K., Hayashi, C., Doi, N., Kitamura, F., Koyama, S., Hata, S., Labeit, S., Yanagida, M., Maeda, T., and Sorimachi, H.
  • Organizer: FASEB Summer Research Conferences - The Biology of Calpains in Health & Disease
  • Place of Presentation: Colorado, USA.
• [Presentation] Novel approaches for analysis of proteolytic activity of muscle-specific calpain p94/calpain 3 (2007)
  • Author(s): Ono, Y., Ojima, K., Hayashi, C., Doi, N., Kitamura, F., Koyama, S., Hata, S., Labeit, S., Yanagida, M., Maeda, T., and Sorimachi, H.
  • Organizer: Xth International Symposium on PROTEINASE INHIBITORS AND BIOLOGICAL CONTROL: From single molecules to degradomics
  • Place of Presentation: Portoroz, Slovenia.
• [Presentation] A novel genetic assay system for a rapidly autolyzing muscle-specific calpain p94/calpain 3 (2006)
  • Author(s): Ono, Y., Torii, F., Ojima, K., Doi, N., Yoshioka, K., Kawabata, Y., Labeit, D., Labeit, S., Suzuki, K., Abe, K., Maeda, T., Hata, S., Koyama, S., and Sorimachi, H.
  • Organizer: 5^<th> Internationla Conference on Cysteine Proteinases and Their Inhitbitors: From Structure to Regulation and Biology
  • Place of Presentation: Portoroz, Slovenia.
• [Presentation] How do defects in proteolytic functions of skeletal muscle-specific calpain, p94/calpain 3, cause calpainopathy/LGMD2A? (2006)
  • Author(s): Sorimachi, H., Ojima, K., Yanagida, M., Ogawa, H., Hayashi, C., Doi, N., and Ono, Y.
  • Organizer: XIth International Congress on Neuromuscular Diseases
  • Place of Presentation: Istanbul, Turkey.
• [Presentation] Novel function of calpain at the membranes. (2006)
  • Author(s): Sorimachi, H., Maeda, T., Suzuki, K.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11^<th> FAOBMB Congress.
  • Place of Presentation: Kyoto, Japan.
• [Remarks] ウェッブサイト
  • URL: http://www.igakuken.or.jp/calpain/
• [Remarks]
  • URL: http://calpain.org/viewer.rb
• [Remarks]
  • URL: http://calpain.net/
• [Remarks]
  • URL: http://www.igakuken.or.jp/calpain/
• [Remarks]
  • URL: http://calpain.org
• [Remarks] 研究室ホームページ:
  • URL: http://www.rinshoken.or.jp/ER/