Three-dimensional super-resolution spectromicroscopy to trace inter-protein interactions and morphological changes in photosynthetic membranes on spatial and temporal domains
| Project Area | Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process |
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| Project/Area Number |
19056012
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2007 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥33,300,000 (Direct Cost: ¥33,300,000)
Fiscal Year 2011: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2010: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2009: ¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 2008: ¥10,100,000 (Direct Cost: ¥10,100,000)
Fiscal Year 2007: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Keywords | シアノバクテリア / チラコイド膜 / 葉緑体 / 顕微分光 / 光化学系 / 2光子励起 / アンチストークス蛍光 / 近赤外レーザー / 蛍光スペクトル / 多光子励起 / 光化学系I / 光化学系II / ヘテロシスト / 蛍光顕微鏡 / クロロフィル / 植物 / 超解像 / 生物物理化学 / 電子・エネルギー移動 / 色素体機能・光合成 / 光計測 / 光合成 / 二光子励起 / 顕微蛍光 / 共焦点顕微鏡 / 緑藻 |
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| Research Abstract |
We have found that photosystem I (PSI)-specific fluorescence spectra of thylakoid membranes of cyanobacteria and chloroplasts was very efficiently observed in the case of continuous wave (CW) near infrared (NIR) excitation around 785-820 nm. It was found that the PSI fluorescence in the case of CW NIR excitation is mainly generated by a one-photon excitation mechanism. This phenomenon will be used for studies of PSI/PSII ratio at physiological temperature, since PSI fluorescence has been conventionally observed at low temperatures like 77 K.
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Report
(7 results)
Research Products
(42 results)
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[Book] Three-Dimensional High-resolution Microspectroscopic Study of Environment-Sensitive Photosynthetic Membranes”(Ed. by H.Fukumura, M.Irie, Y.Iwasawa, H.Masuhara and K.Uosaki)in Molecular Nano dynamics, Volume 2 Chapter 302009
Author(s)
Shigeichi Kumazaki, Makotoh Hasegawa, Mohammad Ghoneim, Takahiko Yoshida, Masahide Terazima, Takashi Shiina, Isamu Ikegam
Publisher
Wiley-VCH
Related Report
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[Book] Molecular Nano Dynamics Vol. 2(H. Fukumura, M. Irie, Y. Iwasawa, H. Masuhara and K. Uosaki eds, Three-Dimensional High-resolution Microspectroscopic Study of Environment-Sensitive Photosynthetic Membranes, Chap. 30)2009
Author(s)
S. Kumazaki, M. Hasegawa, M. Ghoneim, T. Yoshida, M. Terazima, T. Shiina, I. Ikegami
Publisher
Wiley-VCH
Related Report
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[Book] Molecular Nano dynamics, Volume 1, Chapter 16, (Ed. by H. Fukumura, M. Irie, Y. Iwasawa, H. Masuhara and K. Uosaki)2009
Author(s)
S. Kumazaki, M. Hasegawa, M. Ghoneim Yoshida, M. Terazima, T. Shiina, I. Ikegami
Total Pages
20
Publisher
Wiley-VCH
Related Report
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[Book] Photosynthesis. Energy from the Sun, (Ed. by Allen JF, Gantt E, Golbeck JH, and Osmond B.), Volume I, section 2, 93-962009
Author(s)
S. Itoh, T. Uzumaki, S. Takaichi, M. Iwaki, S. Kumazaki, K. Itch, and H. Mino
Total Pages
4
Publisher
Springer, Dordrecht, The Netherlands
Related Report
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[Book] Photosynthesis. Energy from the Sun, (Ed. by Allen JF, Gantt E, Golbeck JH, and Osmond B.), Volume I, section 7. 787-7902009
Author(s)
S. Kumazaki, M. Hasegawa, T. Yoshida, T. Taniguchi, T. Shiina and I. Ikegami
Total Pages
4
Publisher
Springer, Dordrecht, The Netherlands
Related Report
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[Book] A Line-Scanning Multiphoton Fluorescence Spectromicroscope Applied to the Study of the Thylakoid Membrane in Chloroplasts”, Photosynthesis. Energy from the Sun, (Ed. by Allen JF, Gantt E, Golbeck JH, and Osmond B.), Volume I, section 72008
Author(s)
Shigeichi. Kumazaki, Makotoh. Hasegawa, Takahiko Yoshida, Tarou Taniguchi, Takashi Shiina and Isamu Ikegami
Publisher
Springer, Dordrecht, The Netherlands
Related Report
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[Book] Energy from the Sun2008
Author(s)
S. Kumazaki*, M. Hasegawa, T. Yoshida, T. Taniguchi, T. Shiina and I. Ikegami
Total Pages
4
Publisher
Springer
Related Report
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[Book] Molecular Nono Dynamics2008
Author(s)
S. Kumazaki*, M. Hasegawa, M. Ghoneim, T. Yoshida, M. Terazima, T. Shiina, I. Ikegami
Total Pages
20
Publisher
Wiley-VCH
Related Report
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