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Cell cycle regulation of cell morphology and cell size

Planned Research

Project AreaCell Proliferation Control
Project/Area Number 19057002
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

OHYA Yoshikazu  東京大学, 大学院・新領域創成科学研究科, 教授 (20183767)

Co-Investigator(Kenkyū-buntansha) HIRATA Dai  広島大学, 大学院・先端物質科学研究科, 教授 (30243603)
Project Period (FY) 2007 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥79,000,000 (Direct Cost: ¥79,000,000)
Fiscal Year 2011: ¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2010: ¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2009: ¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 2008: ¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 2007: ¥16,200,000 (Direct Cost: ¥16,200,000)
Keywords出芽酵母 / 細胞壁 / 細胞周期 / 細胞形態 / 画像解析 / 形態形成 / 分子遺伝 / 核分裂周期 / 細胞質分裂 / HOG / MAP kinase / シグナル伝達 / 紡錘体 / M期
Research Abstract

Cell wall integrity checkpoint is one of the cell cycle checkpoints in Saccharomyces cerevisiae. When the cell wall synthesis is perturbed, cell cycle progression stopped before nuclear division. We identified 18 genes involved in the cell wall integrity checkpoint. We showed that the checkpoint required the kinase signaling pathway that transduced signal from the cell wall to the nucleus. We also showed that phosphorylation of the transcription factor is also important for regulation of G2/M cyclin, a key regulatory factor of the checkpoint.

Report

(7 results)
  • 2012 Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (48 results)

All 2013 2012 2011 2010 2009 2008 2007 2006 Other

All Journal Article (25 results) (of which Peer Reviewed: 25 results) Presentation (12 results) Remarks (7 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Vanillin Inhibits Translation and Induces Messenger Ribonucleoprotein (mRNP) Granule Formation in Saccharomyces cerevisiae: Application and Validation of High-Content, Image-Based Profiling2013

    • Author(s)
      Iwaki A, Ohnuki S, Suga Y, Izawa S and Ohya Y
    • Journal Title

      PLoS ONE

      Volume: 8(4) Issue: 4 Pages: 61748-61748

    • DOI

      10.1371/journal.pone.0061748

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of the biological activity of a novel 24-membered macrolide JBIR-19 in Saccharomyces cerevisiae by the morpbological imaging program CalMorph2012

    • Author(s)
      Ohnuki S, Kobayashi T, Ogawa H, Kozone I, Ueda JY, Takagi M, Shin-Ya K, Hirata D, Nogami S, Ohya Y
    • Journal Title

      FEMS Yeast Research

      Volume: 3 Issue: 3 Pages: 293-304

    • DOI

      10.1111/j.1567-1364.2011.00770.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ethanol fermentation driven by elevated expression of the G1 cyclin gene CLN3 in sake yeast.2011

    • Author(s)
      Watanabe D
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 112(6) Pages: 577-582

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Cell Wall Integrity Checkpoint: Coordination between Cell Wall Synthesis and the Cell Cycle2010

    • Author(s)
      Negishi T and Ohya Y
    • Journal Title

      Yeast

      Volume: Vol.27 Issue: 8 Pages: 513-519

    • DOI

      10.1002/yea.1795

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple Functional Domains of the Yeast 1,3-β-Glucan Synthase Subunit Fks1p Revealed by Quantitative Phenotypic Analysis of Temperature-Sensitive Mutants Genetics2010

    • Author(s)
      Okada H, Abe M, Asakawa-Minemura M, Hirata A, Qadota H, Morishita K, Ohnuki S, Nogami S, Ohya Y
    • Journal Title

      Mutants Genetics

      Volume: 184(4) Issue: 4 Pages: 1013-24

    • DOI

      10.1534/genetics.109.109892

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple Functional Domains of the Yeast 1,3-β-Glucan Synthase Subunit Fks1p Revealed by Quantitative Phenotypic Analysis of Temperature-Sensitive Mutants Genetics2010

    • Author(s)
      Okada H
    • Journal Title

      Genetics

      Volume: 184(4) Pages: 1013-24

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of bottom-fermenting brewer's yeast KEX2 in high temperature resistance and poor proliferation at low temperatures2010

    • Author(s)
      Yamagishi H
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 4 Pages: 297-312

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-content, image-based screening for drug targets in yeast2010

    • Author(s)
      Ohnuki S
    • Journal Title

      PLoS ONEH

      Volume: 14;5(4)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Knr4 N-terminal domain controls its localization and function during sexual differentiation and vegetative growth2010

    • Author(s)
      Dagkessamanskaia A
    • Journal Title

      Yeast

      Volume: 27(8) Pages: 563-74

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fission yeast GC kinase Ppk11 interacts with Pmo25 and plays an auxiliary role in concert with the morphogenesis Orb6 network (MOR) in cell morphogenesis2010

    • Author(s)
      Goshima T
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 5;285(45) Pages: 35196-205

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple Functional Domains of the Yeast 1, 3-β-Glucan Synthase Subunit Fks1p Revealed by Quantitative Phenotypic Analysis of Temperature-Sensitive Mutants Genetics Epub2010

    • Author(s)
      Okada H, Abe M, Asakawa-Minemura M, Hirata A, Qadota H, Morishita K, Ohnuki S, Nogami S, Ohya Y
    • Journal Title

      (in press)

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth Current Genetics2009

    • Author(s)
      Watanabe M, Watanabe D, Nogami S, Morishita S, Ohya Y
    • Journal Title

      Epub 55(4)

      Pages: 365-80

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Multidimensional quantification of subcellular morphology of Saccharomyces cerevisiae using CalMorph, the high-throughput image-processing program2009

    • Author(s)
      Negishi T
    • Journal Title

      Journal of Biotechnology

      Volume: 141(3-4) Pages: 109-17

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth2009

    • Author(s)
      Watanabe M
    • Journal Title

      Current Genetics

      Volume: 55(4) Pages: 365-80

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel 24-membered macrolides, JBIR-19 and-20 isolated from Metarhiziumsp. fE612009

    • Author(s)
      Kozone, et.al.
    • Journal Title

      Cell Div 62

      Pages: 159-62

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A microfluidic device to acquire high-magnification microphotographs of yeast cells2009

    • Author(s)
      Dhnuki, et.al.
    • Journal Title

      Cell Div 4

      Pages: 5-11

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] G1/S Cyclin-Dependent Kinase Regulates Small GTPase Rho1p through Phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae2008

    • Author(s)
      Kono K, Nogami S, Abe M, Nishizawa M, Morishita S, Pellman D, Ohya Y
    • Journal Title

      Mol Biol Cell

      Volume: 19(4) Pages: 1763-71

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] G1/S Cyclin-Dependent Kinase Regulates Small GTPase Rho1p through Phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae.2008

    • Author(s)
      Kono K, Nogami S, Abe M, Nishizawa M, Morishita S, Pellman D, Ohya Y.
    • Journal Title

      Mol Biol Cell 19(4)

      Pages: 1763-71

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] G1/S cyclin-dependent kinase regulates small GTPase Rholp through phosphorylation of RhoGEF Tusip in Saccharomyces cerevisiae2008

    • Author(s)
      Kono, et.al.
    • Journal Title

      Mol Biol Cell 19

      Pages: 1763-71

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Yvert G Genetic complexity and QTL mapping of yeast morphological traits2007

    • Author(s)
      Nogami S, Ohya Y
    • Journal Title

      Plos Genet

      Volume: 3(2) Issue: 2 Pages: e31-e31

    • DOI

      10.1371/journal.pgen.0030031

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic complexity and QTL mapping of yeast morphological traits.2007

    • Author(s)
      Nogami S, Ohya Y, Yvert G.
    • Journal Title

      PLOS Genet. 3

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Diversity of Ca^<2+>-induced morphology revealed by morphological phenotyping of Ca^<2+>-sensitive mutants of Saccharomyces cerevisiae.2007

    • Author(s)
      Ohnuki S
    • Journal Title

      Eukaryotic Cell 6

      Pages: 817-30

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of small GTPase Rholp in cell size control in yeast.2007

    • Author(s)
      Kikuchi Y
    • Journal Title

      FEMS Yeast Res. 7

      Pages: 569-78

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conditional genomic rearrangement by designed meiotic recombination using VDE (PI-SceI) in yeast.2007

    • Author(s)
      Fukuda T
    • Journal Title

      Mol Genet Genomics 278

      Pages: 467-78

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exploring the Mode-of-Action of Bioactive Compounds by Chemical-Genetic Profiling in Yeast.2006

    • Author(s)
      Parsons, A.B., Lopez, A., Givoni, I.E., Williams, D.E., Gray, C.A., Porter, J., Chua, G., Sopko, R., Brost, R.L., Ho, C.-H., Wang, J., Ketela, T., Brenner, C., Brill, J.A., Fernandez, G.E., Lorenz, T.C., Payne, G.S., Ishihara, S., Ohya Y.,rews, B., Hughes, T.R., Frey, B.J., Graham, T.R.,ersen, R.J., Boone, C.
    • Journal Title

      Cell 126

      Pages: 611-25

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Presentation] Image-based systems biology in the budding yeast Saccharomyces cerevisiae2012

    • Author(s)
      Yoshikazu Ohya
    • Organizer
      ICSB The 13th International Conference on Systems Biology
    • Place of Presentation
      Toronto University (CANADA)
    • Year and Date
      2012-08-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Chemical-genetic analysis on the elucidation of cell wall biogenesis in Saccharomyces cerevisiae2012

    • Author(s)
      Yoshikazu Ohya
    • Organizer
      5th International Conference on Molecular Mechanisms of Fungal Cell Wall Biogenesis
    • Place of Presentation
      Hotel Zora, Kravata Conference Hall (CROATIA)
    • Year and Date
      2012-06-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Quantitative phenotyping of yeast glucan synthase mutants base on the cell morphology2011

    • Author(s)
      Ohya Y
    • Organizer
      2nd Inter- national Fungal Cell Wall Meeting
    • Place of Presentation
      Giens (France)
    • Year and Date
      2011-10-09
    • Related Report
      2012 Final Research Report
  • [Presentation] High content, image based screening of drug targets in yeast2010

    • Author(s)
      大矢禎一
    • Organizer
      The Tokyo University-ENS de Lyon Workshop
    • Place of Presentation
      ENS de Lyonフランス
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 出芽酵母の細胞壁チェックポイントシグナル伝達機構の解明2009

    • Author(s)
      菊地陽
    • Organizer
      第32回日本分子生物学会年会パシフィコ
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Cell wall integrity checkpoint2009

    • Author(s)
      大矢禎一
    • Organizer
      Molecular Mechanisms of Fungal Cell Wall Biogenesis Staszic Palace
    • Place of Presentation
      Historical District of Warsaw
    • Year and Date
      2009-09-02
    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] Chemical genomics to understand cell morphology of Saccharomyces cerevisia2007

    • Author(s)
      Ohya Y
    • Organizer
      Symposium on Systems Biology Initiative
    • Year and Date
      2007-11-18
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Cell wall checkpoint that monitors cell wall integrity in Saccharomyces cerevisiae2007

    • Author(s)
      Ohya Y
    • Organizer
      Fisrt International Symposium on Plant and Yeast Polysaccharides.
    • Place of Presentation
      Angret, France
    • Year and Date
      2007-08-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Dynactin and its interacting molecules are involved in cell wall integrity checkpoint of Saccharomyces cerevisiae2007

    • Author(s)
      Ohya Y
    • Organizer
      2nd Inter- national Workshop on Cell Regulations in Division and Arrest OIST Seaside House
    • Place of Presentation
      (沖縄科学技術大学院大学シーサイドハウス),沖縄
    • Year and Date
      2007-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Dynactin and its interacting molecules are involved in cell wall integrity checkpoint of Saccharomyces cerevisiae2007

    • Author(s)
      Ohya Y
    • Organizer
      Intern at ioal Work shop on Cell Regul at ions in Div is ionand Arrest OIS T Seai d e H o u s e
    • Place of Presentation
      Okinawa
    • Year and Date
      2007-03-23
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Cell wall integrity checkpoint that monitors cell wall remodeling in Saccharomyces cerevisiae2007

    • Author(s)
      Ohya Y
    • Organizer
      The first International Fungal / Plant Cell Wall Meeting Village de Vancances Familiales de Anglet
    • Place of Presentation
      Biarritz (France)
    • Year and Date
      2007-03-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Cell wall integrity checkpoint that monitors cell wall remodeling in Saccharomyces cerevisiae2007

    • Author(s)
      Ohya Y
    • Organizer
      The first International Fungal / Plant Cell Wall Meeting Village de Vancances Familiales de Anglet
    • Place of Presentation
      Biarritz, France
    • Year and Date
      2007-03-12
    • Related Report
      2009 Self-evaluation Report
  • [Remarks]

    • URL

      http://ps.k.u-tokyo.ac.jp/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://ps.k.u-tokyo.ac.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://ps.k.u-tokyo.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://ps.k.u-tokyo.ac.jp/

    • Related Report
      2009 Self-evaluation Report
  • [Remarks]

    • URL

      http://ps.k.u-tokyo.ac.jp/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://ps.k.u-tokyo.ac.jp/top.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://ps.k.u-tokyo.ac.jp/top.html

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞観察装置、細胞観察方法及びそのプログラム2012

    • Inventor(s)
      大矢禎一 他
    • Industrial Property Rights Holder
      JST
    • Filing Date
      2012-11-28
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 細胞形態定量値を用いる酵母の生理状態の評価方法2009

    • Inventor(s)
      大矢禎一、野上 識、大貫慎輔(東京大学)、善本裕之、榎本賢一、堀越杏子(キリンビール 醸造研究所)
    • Industrial Property Rights Holder
      キリンビール
    • Filing Date
      2009-07-27
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 細胞形態定量値を用いる酵母の生理状態の評価方法2009

    • Inventor(s)
      大矢禎一、野上識、大貫慎輔(東京大学)、善本裕之、榎本賢一、堀越杏子(キリンビール醸造研究所)
    • Industrial Property Rights Holder
      キリンビール
    • Filing Date
      2009-07-27
    • Related Report
      2009 Self-evaluation Report
  • [Patent(Industrial Property Rights)] 細胞形態定量値を用いる酵母の生理状態の評価方法2009

    • Inventor(s)
      大矢禎一、野上識、大貫慎輔(東京大学)、善本裕之、榎本賢一、堀越杏子(キリンビール 醸造研究所)
    • Industrial Property Rights Holder
      キリンビール
    • Filing Date
      2009-07-27
    • Related Report
      2009 Annual Research Report

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Published: 2007-04-01   Modified: 2018-03-28  

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