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Advanced microbiobes sepalation and cluturing techniques for post-Koch ecology analysis

Planned Research

Project AreaPost-Koch Ecology: The next-era microbial ecology that elucidates the super-terrestrial organism system
Project/Area Number 19H05680
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Complex systems
Research InstitutionKyushu University

Principal Investigator

Sassa Fumihiro  九州大学, システム情報科学研究院, 准教授 (30722681)

Project Period (FY) 2019-06-28 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥93,730,000 (Direct Cost: ¥72,100,000、Indirect Cost: ¥21,630,000)
Fiscal Year 2023: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2022: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2021: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2020: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
KeywordsBioMEMS / ポストコッホ / ゲル充填マイクロウェルアレイ / マイクロロボット / 微生物 / マイクロフルイディックデバイス / ソフトロボット / 組み合わせ培養 / 組み合わせ培養・分離 / MEMS / 組み合わせ分離・培養
Outline of Research at the Start

現在の微生物学研究で用いられている培養機器は100 年以上前に開発されたものであり、改良の余 地を多く残している。特に未発見の種を含む膨大な微生物種とその生理的機能の多様性の全貌を解明するため には、従来とは桁違いの数のサンプルを処理可能な微生物分離・培養・解析システムの構築が必要で ある。本研究ではマイクロマシンニング技術を用いて、1プレートで 10 万-100 万単位の単離 培養・分析・保存を可能とする次世代の微生物学研究用ツールを構築する。これにより、従来法では 分離できなかった微生物(微生物ダークマター)のスクリーニングおよび、数種類の微生物の相互作用に基づく複合微生物系の一括分析を目指す。

Outline of Final Research Achievements

As part of the development of post-Koch microbial culture technology, we have created 19 different types of gel-filled culture array devices tailored to various microbial species, culture conditions, and analytical applications. Additionally, we have developed ten types of auxiliary tools, such as devices for batch inoculation, and established foundational technology for a microrobot designed for microbial culture array operations (Patent No. 7460053, US Patent Application: US.2021388299.A1). Collaborative research with other project teams has led to significant advancements, including the discovery of new microbial species using these devices. We have compiled the processing methods and design data for all developed devices into a comprehensive library and created a standard experimental procedure manual for these microculture devices, ensuring they are easy to use for anyone.

Academic Significance and Societal Importance of the Research Achievements

従来にない高効率の微生物の培養・分離操作の実現を目的とし、微細加工技術を用いたポストコッホ培養分離ツール群の開発を行った。このデバイスは従来のシャーレを用いた培養と比べ1/5000程度の体積で同等の数の個別培養を可能とする。このデバイスを実証するために領域内を含む国内外の共同研究先で実証実験を行いその有効性、ツールとしての操作性・実用性を実証した。また、この培養アレイを操作するための多数の培養チャンバーを一括して操作する補助ツールの開発やマイクロロボット技術と組み合わせた新規培養操作技術など次世代の大規模集積微生物培養技術の基礎技術を構築した。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (29 results)

All 2024 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (7 results) Journal Article (11 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 11 results,  Open Access: 7 results) Presentation (9 results) (of which Int'l Joint Research: 9 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] University of Pau and Pays de l'Adour(フランス)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] University of California San Diego(米国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] University of Pau and Pays de l'Adour(フランス)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Southwest University(中国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] University of California San Diego(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Southwest University(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Shahjalal University(バングラデシュ)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Two-Dimensional SERS Sensor Array for Identifying and Visualizing the Gas Spatial Distributions of Two Distinct Odor Sources2024

    • Author(s)
      Chen Lin、Guo Hao、Wang Cong、Chen Bin、Sassa Fumihiro、Hayashi Kenshi
    • Journal Title

      Sensors

      Volume: 24 Issue: 3 Pages: 790-790

    • DOI

      10.3390/s24030790

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Visualization of the Gas Spatial Distribution of the Odor Source by SERS Gas Sensor2024

    • Author(s)
      Chen Lin、Guo Hao、Matsuo Takuya、Sassa Fumihiro、Hayashi Kenshi
    • Journal Title

      IEEJ Transactions on Electrical and Electronic Engineering

      Volume: 19 Issue: 5 Pages: 876-881

    • DOI

      10.1002/tee.23988

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Active Optical Sensor Microrobot Equipped With Multi-DoF Gripper Arm Based on Kinetic Electronics2023

    • Author(s)
      Otsuka Riku、Zhang Shiyi、Hayashi Kenshi、Sassa Fumihiro
    • Journal Title

      IEEE Sensors Letters

      Volume: 7 Issue: 9 Pages: 1-4

    • DOI

      10.1109/lsens.2023.3303076

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An Odor Trace Visualization System Using a Two-Dimensional Backside Scattering Localized Surface Plasmon Resonance Gas Sensor2023

    • Author(s)
      Yang Zhongyuan、Sassa Fumihiro、Hayashi Kenshi
    • Journal Title

      Sensors

      Volume: 23 Issue: 23 Pages: 9525-9525

    • DOI

      10.3390/s23239525

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatiotemporal Visualization of Gases Using 2-D LSPR Gas Sensor2023

    • Author(s)
      Matsuoka Masato、Lingpu Ge、Sassa Fumihiro、Hayashi Kenshi
    • Journal Title

      IEEE Sensors Letters

      Volume: 7 Issue: 9 Pages: 1-4

    • DOI

      10.1109/lsens.2023.3301847

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Subpixel Patterned LSPR Gas Sensor Array With Using Inkjet Printing Au/Ag Nanoparticle to Enhance the Selectivity2023

    • Author(s)
      Jiang Tianshu、Ye Xiao、Ge Lingpu、Guo Hao、Sassa Fumihiro、Hayashi Kenshi
    • Journal Title

      IEEE Sensors Letters

      Volume: 7 Issue: 9 Pages: 1-4

    • DOI

      10.1109/lsens.2023.3300820

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a microfluidic-based taste sensor using lipid polymer membrane2023

    • Author(s)
      Tahara Yusuke、Sassa Fumihiro、Takigawa Ryo、Kurihara Yuma
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 62 Issue: SG Pages: SG1014-SG1014

    • DOI

      10.35848/1347-4065/acb4fa

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low-cost gel-filled microwell array device for screening marine microbial consortium2022

    • Author(s)
      Clelia Duran, Shiyi Zhang, Chongyang Yang, Maria Lorena Falco, Cristiana Cravo-Laureau, Chiho Suzuki-Minakuchi, Hideaki Nojiri, Robert Duran, Fumihiro Sassa
    • Journal Title

      Frontiers in Microbiology

      Volume: 13 Pages: 01-09

    • DOI

      10.3389/fmicb.2022.1031439

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A fully inkjet-printed disposable gas sensor matrix with molecularly imprinted gas-selective materials2022

    • Author(s)
      Ge Lingpu、Ye Xiao、Yu Zeping、Chen Bin、Liu Chuanjun、Guo Hao、Zhang Shiyi、Sassa Fumihiro、Hayashi Kenshi
    • Journal Title

      npj Flexible Electronics

      Volume: 6 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41528-022-00168-6

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chemiresistor sensor matrix prepared by full-printing processes2021

    • Author(s)
      Ge Lingpu、Ye Xiao、Chen Bin、Liu Chuanjun、Guo Hao、Sassa Fumihiro、Hayashi Kenshi
    • Journal Title

      Flexible and Printed Electronics

      Volume: 6 Issue: 1 Pages: 015013-015013

    • DOI

      10.1088/2058-8585/abec19

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microfabricated electrochemical sensing devices2020

    • Author(s)
      F. Sassa, G. C. Biswas, and H. Suzuki
    • Journal Title

      Lab Chip

      Volume: 20 Issue: 8 Pages: 1358-1389

    • DOI

      10.1039/c9lc01112a

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 2D SERS Gas Sensor for Visualization and Localization of the Odor Source2023

    • Author(s)
      Chen Lin、Wang Cong、Guo Hao、Sassa Fumihiro、Hayashi Kenshi
    • Organizer
      2023 IEEE SENSORS
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication of Biosensing Film Robot Based on Kinetic Electronics2022

    • Author(s)
      Shiyi Zhang , Kenshi Hayashi , Joseph Wang , Fumihiro Sassa
    • Organizer
      Advances in Functional Materials2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Odor Source Detection with High Speed Multi Gas Sensing Robot System using AuNPs-Fluorescent Molecular coupling Opt-Chemical LSPR Sensor2020

    • Author(s)
      Y Kusuda, Z Yang, K. Semas, F Sassa, K Hayashi
    • Organizer
      2020 IEEE Sensors
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ultra-high Sensitive SERS Gas Sensor to detect Geosmin2020

    • Author(s)
      Lin Chen, Noriko Shiramatsu, Bin Chen, Fumihiro Sassa, Shoichi Sameshima, Tatsuya Seki, Kenshi Hayashi
    • Organizer
      2020 IEEE Sensors
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 2D LSPR gas sensor with Au/Ag core-shell structure coated by fluorescent dyes2020

    • Author(s)
      K Semasa, F Sassa, K Hayashi
    • Organizer
      2020 IEEE Sensors
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Invisible Odor Trace Tracking with LSPR based High Speed Gas Sensor Robot System2019

    • Author(s)
      Y Kusuda, Z Yang, T Soeda, F Sassa, K Hayashi
    • Organizer
      18th IEEE Sensors, SENSORS 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Inkjet-printed Gas Sensor Matrix with Molecularly Imprinted Gas Selective Materials2019

    • Author(s)
      L Ge, B Chen, H Kawano, F Sassa, K Hayashi
    • Organizer
      18th IEEE Sensors, SENSORS 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multi-layer Filter Structure for Molecular Selective SERS Gas Sensor2019

    • Author(s)
      L Chen, B Chen, F Sassa, K Hayashi
    • Organizer
      18th IEEE Sensors, SENSORS 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 2D LSPR multi gas sensor array with 4-segmented subpixel using Au/Ag core shell structure2019

    • Author(s)
      T Soeda, Z Yang, F Sassa, Y Tomiura, K Hayashi
    • Organizer
      18th IEEE Sensors, SENSORS 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Remarks] ポストコッホ生態

    • URL

      https://postkoch.jp/

    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 操作装置及び操作方法2020

    • Inventor(s)
      佐々文洋, 武居 修, 茂木一
    • Industrial Property Rights Holder
      佐々文洋, 武居 修, 茂木一
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-102019
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2019-07-04   Modified: 2025-01-30  

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