|Budget Amount *help
¥71,630,000 (Direct Cost: ¥55,100,000、Indirect Cost: ¥16,530,000)
Fiscal Year 2012: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2011: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2010: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2009: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2008: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
For elucidating the functions of ROS and RNS as intracellular signaling molecules, we aimed at developing novel optical imaging probes which afford their live-imaging in living biological samples. We have succeeded in developing fluorescence probes for hydrogen peroxide, acrolein, and the enzymatic activity of glutathione S-transferase, those for highly ROS and NO which has much desired property of intracellular retention for the long-period observation, that for hypochlorite with NIR emitting capability, and luminescence probes for detecting hROS. We also have succeeded to obtain various striking images of each ROS and RNS in living cells and animals.