|Budget Amount *help
¥43,810,000 (Direct Cost : ¥33,700,000、Indirect Cost : ¥10,110,000)
Fiscal Year 2012 : ¥9,620,000 (Direct Cost : ¥7,400,000、Indirect Cost : ¥2,220,000)
Fiscal Year 2011 : ¥9,490,000 (Direct Cost : ¥7,300,000、Indirect Cost : ¥2,190,000)
Fiscal Year 2010 : ¥9,490,000 (Direct Cost : ¥7,300,000、Indirect Cost : ¥2,190,000)
Fiscal Year 2009 : ¥9,490,000 (Direct Cost : ¥7,300,000、Indirect Cost : ¥2,190,000)
Fiscal Year 2008 : ¥5,720,000 (Direct Cost : ¥4,400,000、Indirect Cost : ¥1,320,000)
We investigated the roles of water and ATP in the functional mechanism of ABC transporter and obtained the following conclusions: 1) the hydrolysis energies of ATP and its related compounds all converge to ~10 kcal/mol by the effect of hydration, 2) the main driving force of the protein-ligand complexation is the translational entropy of water (exclusion volume effect), 3) the binding-free energy of ATP can be converted to a power stroke like the dimerization of NBDs, 4) a long-range hydration force of enthalpic origin and the water-mediated attraction of entropic origin drive the NBD dimerization in ABC transporter.