Regulation of NF-kB signaling by post-translational modifications and its relation to disease onsets

• Project Area: Regulation of signal transduction by post-translational modifications and its pathogenic dysregulation
• Project/Area Number: 22117002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: Inoue Jun-ichiro 東京大学, 医科学研究所, 教授 (70176428)
• Co-Investigator(Kenkyū-buntansha): OYAMA Masaaki 東京大学, 医科学研究所, 准教授 (30422398)
• Co-Investigator(Renkei-kenkyūsha): GOHDA Jin 東京大学, 医科学研究所, 特任講師 (90361617) ; YAMAGUCHI Noritaka 千葉大学, 大学院薬学研究院, 助教 (80399469) ; TAGUCHI Yuu 東京大学, 医科学研究所, 助教 (20549472)
• Project Period (FY): 2010-04-01 – 2016-03-31
• Project Status: Completed(Fiscal Year 2015)
• Budget Amount: ¥310,700,000 (Direct Cost : ¥239,000,000、Indirect Cost : ¥71,700,000); Fiscal Year 2014 : ¥50,180,000 (Direct Cost : ¥38,600,000、Indirect Cost : ¥11,580,000); Fiscal Year 2013 : ¥56,680,000 (Direct Cost : ¥43,600,000、Indirect Cost : ¥13,080,000); Fiscal Year 2012 : ¥54,080,000 (Direct Cost : ¥41,600,000、Indirect Cost : ¥12,480,000); Fiscal Year 2011 : ¥56,550,000 (Direct Cost : ¥43,500,000、Indirect Cost : ¥13,050,000); Fiscal Year 2010 : ¥93,210,000 (Direct Cost : ¥71,700,000、Indirect Cost : ¥21,510,000)
• Keywords: 翻訳後修飾 / シグナル伝達 / 疾患 / 数理モデル / 構造生物学 / ユビキチン / 癌 / 質量分析

Outline of Final Research Achievements

1. p47 inhibits NF-kB activation by inducing degradation of NEMO in lysosome. 2. NF-kB activation by HTLV-1 Tax protein requires generation of K63-linked polyubiquitin chains. 3. NF-kB activation in non-cancer stem cells induces expression of JAG1, which in turn stimulates cancer stem cells to increase stem cell population. NF-kB activation also induces expression of TMOD1, which leads to induction of MMP13, a protease that degrades extracellular matrix. 4. RelB-Venus knock-in mice is a tool to analyze spatiotemporal regulation of RelB.

Research Products

• [Journal Article] Targeting the vacuolar proton pump and acid-sensing ion channel 3 decreses multiple myeloma-induced bone pain. (2017)
  • Author(s): Hiasa M, Okui T, Allette YM, Ripsch MS, Sun-Wada G-H, Yoneda T.
  • Journal Title: Cancer Res. Volume : 77 Pages : 1283-1295
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Integrative Network Analysis Combined with Quantitative Phosphoproteomics Reveals TGFBR2 as a Novel Regulator of Glioblastoma Stem Cell Properties (2016)
  • Author(s): Narushima Y, Kozuka-Hata H, Koyama-Nasu R, Tsumoto K, Inoue J, Akiyama T & Oyama M.
  • Journal Title: Molecular and Cellular Proteomics Volume : 15 Pages : 1017-1031
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Quantitative phosphoproteomics-based molecular network description for high-resolution kinase-substrate interactome analysis. (2016)
  • Author(s): Narushima Y, Kozuka-Hata H, Tsumoto K, Inoue J, Oyama M.
  • Journal Title: Bioinformatics Volume : 印刷中 Pages : 2083-2088
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Excess NF-kB induces ectopic odontogenesis in embryonic incisor epithelium. (2015)
  • Author(s): Blackburn, J., Kawasaki, K., Porntaveetus, T., Kawasaki, M., Otsuka-Tanaka, Y., Miake, Y., Ota, M.S., Watanabe, M., Hishinuma, M., Nomoto, T., Oommen, S., Ghafoor, S., Harada, F., Nozawa-Inoue, K., Maeda, T., Peterková, R., Lesot, H., Inoue, J., Akiyama, T.,
  • Journal Title: J. Dent. Res Volume : 94 Pages : 121-128
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structures of CYLD USP with Met1- or Lys63-linked diubiquitin reveal mechanisms for dual specificity (2015)
  • Author(s): Sato, Y., Goto, E., Shibata, Y., Kubota, Y., Yamagata, A., Goto-Ito, S., Kubota, K., Inoue, J., Takekawa, M., Tokunaga, F. and Fukai, S.
  • Journal Title: Nat. Struct. Mol. Biol. Volume : 22 Pages : 222-229
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] HIV-1 replicates in human osteoclasts and enhances their differentiation in vitro. (2015)
  • Author(s): Gohda, J., Ma, Y., Huang, Y., Zhang, Y., Gu, L., Han, Y., Li, T., Gao, G.F., Inoue, J., Iwamoto, A. and Ishida, T.
  • Journal Title: Retrovirology Volume : 12 Pages : 12-12
  • Peer Reviewed / Open Access
• [Journal Article] Catalytic subunits of the phosphatase calcineurin interact with NF-B-inducing kinase (NIK) and attenuate NIK-dependent gene expression. (2015)
  • Author(s): Shinzawa. M., Konno, H., Qin, J., Akiyama, N., Miyauchi, M., Ohashi, H., Miyamoto-Sato, E., Yanagawa, H., Akiyama, T.* and Inoue, J.
  • Journal Title: Sci. Rep Volume : 5 Pages : 10758-10758
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Visualization of RelB expression and activation at the single-cell level during dendritic cell maturation in Relb-Venus knock-in mice (2015)
  • Author(s): Takao Seki, Mami Yamamoto, Yuu Taguchi, Maki Miyauchi, Noritaka Yamaguchi, Jin Gohda, Taishin Akiyama, Jun-ichiro Inoue
  • Journal Title: Journal of Biochemistry Volume : 158 Pages : 485-495
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Loss of Tifab, a del(5q) MDS gene, alters hematopoiesis through derepression of Toll-like receptor-TRAF6 signaling (2015)
  • Author(s): Varney, M.E., Niederkorn, M., Konno, H., Matsumura, T., Gohda, J., Yoshida, N., Akiyama, T., Christie, S., Fang, J., Miller, D., Jerez, A., Karsan, A., Maciejewski, J.P., Inoue, J. and Starczynowski, D.T
  • Journal Title: J. Exp. Med Volume : 212 Pages : 1967-1985
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Mitochondria-nucleus shuttling FK506-binding protein 51 interacts with TRAF proteins and facilitates the RIG-I-like receptor-mediated expression of type I IFN (2014)
  • Author(s): Akiyama, T., Shiraishi, T., Qin, J., Konno, H., Akiyama, N., Shinzawa, M., Miyauchi, M., Takizawa, N., Yanai, H., Ohashi, H., Miyamoto-Sato, E., Yanagawa, H., Yong, W., Shou, W. and Inoue, J.
  • Journal Title: PLoS ONE Volume : 9(5)
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizations. (2014)
  • Author(s): Kumeta, M., Gilmore, J. L., Umeshima, H., Ishikawa, M., Kitajiri, S. I., Horigome, T., Kengaku, M., Takeyasu, K.
  • Journal Title: Genes Cells Volume : 19 Pages : 338-349
  • Peer Reviewed
• [Journal Article] NF-κB non-cell-autonomously regulates cancer stem cell populations in the basal-like breast cancer subtype. (2013)
  • Author(s): Yamamoto, M
  • Journal Title: Nat. Commun. Volume : 4 Pages : 2299-2299
  • Peer Reviewed
• [Journal Article] Involvement of A20 in the molecular switch that activates the non-canonical NF-κB pathway. (2013)
  • Author(s): Yamaguchi, N
  • Journal Title: Sci. Rep Volume : 3 Pages : 2568-2568
  • Peer Reviewed
• [Journal Article] Partial purification of Lys63 ubiquitinated proteins by using a column switch HPLC method. (2013)
  • Author(s): Kojima, A
  • Journal Title: PEPTIDE SCIENCE 2012 Volume : 2012 Pages : 161-164
  • Peer Reviewed
• [Journal Article] p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO. (2012)
  • Author(s): Shibata Y, Oyama M, Kozuka-Hata H, Han X, Tanaka Y, Gohda J, and Inoue J.
  • Journal Title: Nat Commun Volume : 3 Pages : 1061-1061
  • Peer Reviewed
• [Journal Article] Roles of spatial parameters on the oscillation of nuclear NF-#61547;B: computer simulations of a 3D spherical cell. (2012)
  • Author(s): Oshima, D
  • Journal Title: PLoS ONE Volume : 7
  • Peer Reviewed
• [Journal Article] cIAP1/2 negatively regulate RANKL-induced osteoclastogenesis through the inhibition of NFATc1 expression (2012)
  • Author(s): Noritaka Yamaguchi
  • Journal Title: Genes to Cells Volume : 17 Pages : 971-981
  • Peer Reviewed
• [Journal Article] Identification of interleukin-1 receptor-associated kinase 1 as a critical component that induces post-transcriptional activation of IκB-ζ (2012)
  • Author(s): Ohba T, Ariga Y, Maruyama T, Truong NK, Inoue J, Muta T
  • Journal Title: FEBS J Volume : 279(2) Pages : 211-22
  • Peer Reviewed
• [Journal Article] The Shigella flexneri effector OspI deamidates UBC13 to dampen the inflammatory response (2012)
  • Author(s): Sanada, T.
  • Journal Title: Nature Volume : 483 Pages : 623-626
  • Peer Reviewed
• [Journal Article] Identification and characterization of anti-osteoclastogenic peptides derived from the cytoplasmic tail of RANK (2012)
  • Author(s): Taguchi, Y.
  • Journal Title: J.Bone Miner.Metab. Volume : (in press)
  • Peer Reviewed
• [Journal Article] ErbB/NF-kB signaling controls mammosphere formation in human breast cancer (2012)
  • Author(s): Hinohara, K.
  • Journal Title: Proc.Natl.Acad.Sci Volume : (in press) Pages : 6584-6589
  • Peer Reviewed
• [Journal Article] RANK signaling induces interferon-stimulated genes inthe fetal thymic (2011)
  • Author(s): Ohshima D., Qin J., Konno H., Hirosawa A., Shiraishi T., Yanai H., Shimo Y., Shinzawa M., Akiyama N., Yamashita R., Nakai K., Akiyama T*(corresponding author), and
  • Journal Title: Biochem. Biophys. Res. Commun. Volume : 408 Pages : 530-536
  • Peer Reviewed
• [Journal Article] Activation of the IkB kinase complex by HTLV-1 Tax requires cytosolic factors involved in Tax-induced polyubiquitination (2011)
  • Author(s): Shibata, Y.
  • Journal Title: J.Biochem. Volume : 150 Pages : 679-686
  • Peer Reviewed
• [Journal Article] Splenic extramedullary hemopoiesis caused by a dysfunctional mutation inthe (2011)
  • Author(s): Shinzawa M., Maruyama Y., Qin J., Akiyama N., Miyauchi M., Yanai H., Takami M., Inoue J., and Akiyama T*(corresponding author).
  • Journal Title: Biochem. Biophys. Res. Commun. Volume : 414 Pages : 773-778
  • Peer Reviewed
• [Journal Article] TRAF6 directs commitment to regulatory T cells in thymocytes (2011)
  • Author(s): Shimo.Y
  • Journal Title: Genes Cells Volume : 16 Pages : 437-447
  • Peer Reviewed
• [Journal Article] Lymphotoxin Signal Promotes Thymic Organogenesis by Eliciting RANK Expression in the Embryonic Thymic Stroma. (2011)
  • Author(s): Mouri.Y
  • Journal Title: J.Immunol. Volume : (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] RANK signaling induces interferon-stimulated genes in the fetal thymic stroma. (2011)
  • Author(s): Ohshima.D
  • Journal Title: Biochem.Biophys.Res.Commun. Volume : (in press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Epigenetic alteration of the NF-κB inducing kinase (NIK) gene is involved in enhanced NIK expression in basal-like breast cancer. (2010)
  • Author(s): Yamamoto.M
  • Journal Title: Cancer Sci. Volume : 101 Pages : 2391-2397
  • Peer Reviewed
• [Journal Article] Identification of BCAP-L as a negative regulator of the TLR signaling-induced production of IL-6 and IL-10 in macrophages by tyrosine phosphoproteomics. (2010)
  • Author(s): Matsumura.T
  • Journal Title: Biochem.Biophys.Res.Commun. Volume : 400 Pages : 265-270
  • Peer Reviewed
• [Journal Article] TRAF6 negatively regulates the Jak1-Erk pathway in interleukin-2 signaling. (2010)
  • Author(s): Motegi.H
  • Journal Title: Genes Cells Volume : 16 Pages : 179-189
  • Peer Reviewed
• [Presentation] Visualization of RelB expression and activation at the single-cell level during dendritic cell maturation in Relb-Venus knock-in mice (2016)
  • Author(s): 関崇生、山本真実、田口祐、宮内真紀、秋山伸子、山口憲孝、合田仁、秋山泰身、井上純一郎
  • Organizer: American Association for Cancer Research
  • Place of Presentation: Whistler Conference Centre（カナダ バンクーバー）
  • Year and Date: 2016-03-13
  • Int'l Joint Research
• [Presentation] Analysis of HTLV-1 Tax-induced IKK activation pathway (2016)
  • Author(s): 柴田佑里、井上純一郎
  • Organizer: American Association for Cancer Research
  • Place of Presentation: Whistler Conference Centre（カナダ バンクーバー）
  • Year and Date: 2016-03-13
  • Int'l Joint Research
• [Presentation] TRAF6 regulates pregnancy-induced mammary gland development and maintenance of epithelial stem cells (2016)
  • Author(s): 山本瑞生、井上純一郎
  • Organizer: Keystone symposia Stem Cells and Cancer (C1)
  • Place of Presentation: Beaver Run Resort（米国コロラド州）
  • Year and Date: 2016-03-06
  • Int'l Joint Research
• [Presentation] Stimulation-dependent internalization of RANK is crucial for differentiation of osteoclast involved in bone metastasis (2016)
  • Author(s): 田口祐、井上純一郎
  • Organizer: Breakthroughs in Cancer Research:from Biology to Therapeutics
  • Place of Presentation: Hyatt Regency Maui（米国ハワイ州）
  • Year and Date: 2016-02-16
  • Int'l Joint Research
• [Presentation] 非古典的NF-κB経路の振動は標的遺伝子の転写を制御する (2015)
  • Author(s): 関崇生、田口祐、秋山泰身、井上純一郎
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 妊娠期の乳癌成熟および幹細胞維持におけるTRAF6の役割 (2015)
  • Author(s): 山本瑞生、井上純一郎
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 転写因子NF-κBの制御メカニズムとその生理機能 (2015)
  • Author(s): 井上純一郎
  • Organizer: 数理医学研究会 第51回数理医学セミナー
  • Place of Presentation: 大阪大学（大阪府豊中市）
  • Year and Date: 2015-11-25
• [Presentation] Regulation of the TRAF-NF-κB and the Calcineurin-NFATc1 pathways in RANK signal-induced osteoclastogenesis (2015)
  • Author(s): 井上純一郎
  • Organizer: East Asia joint symposium
  • Place of Presentation: Oist Campus（沖縄県国頭郡）
  • Year and Date: 2015-11-11
  • Int'l Joint Research
• [Presentation] 乳癌における細胞間相互作用による上皮感葉転換の制御 (2015)
  • Author(s): 山本瑞生、井上純一郎
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際センター（愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] HTLV-1Tax誘導性のIKK複合体活性化におけるポリユビキチン鎖の機能解析 (2015)
  • Author(s): 柴田佑里、井上純一郎
  • Organizer: 日本HTLV-1学会学術集会
  • Place of Presentation: 東京大学医科学研究所（東京都港区）
  • Year and Date: 2015-08-22
• [Presentation] 刺激依存的な受容体RANKの細胞質内局在化が果たす破骨細胞分化における役割 (2015)
  • Author(s): 田口祐、井上純一郎
  • Organizer: 日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-07-23
• [Presentation] 破骨細胞の分化成熟におけるTRAF6の役割 (2015)
  • Author(s): 弓桁洋、田口祐、井上純一郎
  • Organizer: 日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-07-23
• [Presentation] Lys63 型ポリユビキチン鎖に特異的なアフィニティカラムの調製 (2015)
  • Author(s): 玉城裕也、小嶋絢、山村広斗、永井裕子、豊田英尚、合田仁、井上純一郎、谷口将済、小西元美、秋澤俊史
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学（兵庫県神戸市）
  • Year and Date: 2015-03-25
• [Presentation] NF-κBシグナルによるTriple negative乳がんの悪性化 (2015)
  • Author(s): 井上純一郎
  • Organizer: 癌研究分野の特性等を踏まえた支援活動 公開シンポジウム
  • Place of Presentation: 一橋講堂 学術総合センター（東京都千代田区）
  • Year and Date: 2015-01-27
• [Presentation] A Proteomic Approach to Identify Novel Factors Involved in the STING-mediated Cytosolic Viral DNA Sensing (2014)
  • Author(s): 山本 真実
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infection
  • Place of Presentation: 仙台インターナショナルセンター
• [Presentation] lB-venus knock-in mice as a powerful tool of studying dynamic regulation of the non-canonical NF-kB activation (2014)
  • Author(s): 関 崇生
  • Organizer: lB-venus knock-in mice as a powerful tool of studying dynamic regulation of the non-canonical NF-kB activation
  • Place of Presentation: キーストンリゾート アメリカ コロラド
• [Presentation] Identification and analysis of a novel regulatory factor involved in HTLV-1 Tax-induced IKK activation (2014)
  • Author(s): 柴田 佑里
  • Organizer: lB-venus knock-in mice as a powerful tool of studying dynamic regulation of the non-canonical NF-kB activation
  • Place of Presentation: キーストンリゾート アメリカ コロラド
• [Presentation] NF-κB活性化の時空間的制御機構の解明 (2014)
  • Author(s): 井上純一郎
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25
• [Presentation] 受容体RANKの刺激依存的な細胞質内への局在化と、その破骨細胞分化における役割 (2014)
  • Author(s): 田口祐、井上純一郎
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25
• [Presentation] RANKL誘導性NFATc1の発現促進を介したp47の破骨細胞分化への関与 (2014)
  • Author(s): 韓笑、柴田佑里、井上純一郎
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25
• [Presentation] HTLV-1Tax誘導性のIKK複合活性化に必要なユビキチンりガーゼの同定 (2014)
  • Author(s): 柴田佑里、井上純一郎
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25
• [Presentation] 非古典的NF-κB経路の振動による転写制御メカニズムの解析 (2014)
  • Author(s): 関崇生、大島大輔、田口祐、秋山泰身、市川一寿、井上純一郎
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25
• [Presentation] Tropomodulin 1 expression driven by NF-kB enhances breast cancer growth (2014)
  • Author(s): 井上純一郎
  • Organizer: The 4th Japan-France Cancer Workshop
  • Place of Presentation: 関西セミナーハウス（京都府京都市）
  • Year and Date: 2014-11-19
  • Int'l Joint Research / Invited
• [Presentation] 癌骨転移に関与するは骨細胞における受容体RANKの細胞内取り込みとその機能 (2014)
  • Author(s): 田口祐、井上純一郎
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-25
• [Presentation] HTLV-1 Tax誘導性のIKK複合体活性化に必要なユビキチンりガーゼの同定 (2014)
  • Author(s): 柴田佑里、井上純一郎
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-25
• [Presentation] The stimulartion-dependent internalization of RANK is crucial for osteoclast differentiation (2014)
  • Author(s): 田口祐、井上純一郎
  • Organizer: The American Society for Bone and Mineral Research
  • Place of Presentation: George R. Brown Convention Center（米国テキサス州）
  • Year and Date: 2014-09-12
• [Presentation] NF-κB, a key player in breast cancer development (2014)
  • Author(s): 井上純一郎
  • Organizer: 1st Symposium of SPU Innovative Project for Pharmaceutical Analyses of Covalent Modification in Biomolecules
  • Place of Presentation: 昭和薬科大学（東京都町田市）
  • Year and Date: 2014-08-28
  • Invited
• [Presentation] HTLV-1 TaxによるIKK複合体活性化に必要な因子の同定と解析 (2014)
  • Author(s): 柴田佑里、尾山大明、秦裕子、井上純一郎
  • Organizer: 日本HTLV-1学会学術集会
  • Place of Presentation: 東京大学医科学研究所（東京都港区）
  • Year and Date: 2014-08-22
• [Presentation] RANK Harbors the Specific Cytoplasmic Domain that Regulates Osteoclastogenic Signaling and Its Subcellular Localization (2013)
  • Author(s): 田口 祐
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
  • Place of Presentation: 神戸国際会議場
• [Presentation] Genetic Screening of Novel Negative Regulators in Osteoclast Differentation (2013)
  • Author(s): 白井 福寿
  • Organizer: RANK Harbors the Specific Cytoplasmic Domain that Regulates Osteoclastogenic Signaling and Its Subcellular Localization
  • Place of Presentation: 神戸国際会議場
• [Presentation] Identification of Proteins Binding to the Unique Domain in RANK Required to Establish Osteoclastogenic Signals (2013)
  • Author(s): 津村 和明
  • Organizer: RANK Harbors the Specific Cytoplasmic Domain that Regulates Osteoclastogenic Signaling and Its Subcellular Localization
  • Place of Presentation: 神戸国際会議場
• [Presentation] 翻訳後修飾によるNF-κB活性化シグナルの制御と疾患発症との関連 (2013)
  • Author(s): 井上 純一郎
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインクあいち
• [Presentation] 核と細胞質の間で振動する転写因子NF-κBの活性制御 (2013)
  • Author(s): 井上 純一郎
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] HTLV-1 TaxによるIKK複合体活性化に必要な新規タンパク質の同定と解析 (2013)
  • Author(s): 柴田 佑里
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 受容体RANKの機能領域HCRによるRANKの細胞内取り込みとその破骨細胞分化への関与 (2013)
  • Author(s): 田口 祐
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Molecular mechanisms of osteoclastogenic signal (2013)
  • Author(s): 井上 純一郎
  • Organizer: 3rdGDRI French Japanese Cancer Meeting
  • Place of Presentation: フランス トゥールーズ
• [Presentation] 受容体RANKのRANKL刺激依存的な細胞内取り込みとその破骨細胞分化における役割 (2013)
  • Author(s): 田口 祐
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] HTLV-1 TaxによるIKK複合体活性化に必要な新規タンパク質の同定と解析 (2013)
  • Author(s): 柴田 佑里
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 初代培養乳腺上皮細胞を用いた3次元培養法の確立とその発がん機構解析への応用 (2013)
  • Author(s): 山本 瑞生
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Ubiquitin E3 ligases cIAP1/2 negatively regulate osteoclastogenesis (2013)
  • Author(s): 山口 憲孝
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-03-27
• [Presentation] Critical roles of NF-kB activation in breast cancer development (2013)
  • Author(s): 井上 純一郎
  • Organizer: Breakthroughs in Basic and Translational Cancer Research
  • Place of Presentation: ハイアットリージェンシーマウイ アメリカ ハワイ州
  • Year and Date: 2013-02-21
• [Presentation] Preparation of the K63-linked polyyubiquitin-specific affinity columun packed with the Zinc Finger peptide derived frm TAK1 binding protein2 (2013)
  • Author(s): 小嶋 絢
  • Organizer: Breakthroughs in Basic and Translational Cancer Research
  • Place of Presentation: ハイアットリージェンシーマウイ アメリカ ハワイ州
  • Year and Date: 2013-02-21
• [Presentation] A non-cell-autonomous role of NF-kappaB inregulating cancer stem cell populations in the basal-like breast cancer subtype (2013)
  • Author(s): 山本 瑞生
  • Organizer: がん研究分野の特性等を踏まえた支援活動・公開シンポジウム
  • Place of Presentation: 学術総合センター 一橋講堂
  • Year and Date: 2013-01-29
• [Presentation] Activation of the IκB kinase complex by HTLV-1 Tax requires cytosolic factors involved in Tax-induced polyubiquitination (2013)
  • Author(s): 柴田 佑里
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2013
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2013-01-23
• [Presentation] Critical roles of NF-kB activation in breast cancer development (2012)
  • Author(s): Jun-ichiro Inoue
  • Organizer: TGF-β Family : Signal Nwtwork in Biological Functions
  • Place of Presentation: University of Tokyo, Koshiba Hall
• [Presentation] Identification and characterization of a novel inhibitory factor in NF-kB activation pathway (2012)
  • Author(s): Yuri Shibata
  • Organizer: 2012 KEYSTONE SYMPOSIA
  • Place of Presentation: Whistler Conference Centre Canada
• [Presentation] Functional analysis of clAP1/2 in RANK signaling in osteoclast precursor cells (2012)
  • Author(s): Noritaka Yamaguchi
  • Organizer: 2012 KEYSTONE SYMPOSIA
  • Place of Presentation: Whistler Conference Centre Canada
• [Presentation] Paracrine role of transcription factor NF-kB in maintenance of breast cancer tumor intiating cells (2012)
  • Author(s): Mizuki Yamamoto
  • Organizer: 2012 KEYSTONE SYMPOSIA
  • Place of Presentation: Whistler Conference Centre Canada
• [Presentation] p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO (2012)
  • Author(s): 柴田 佑里
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] Indentificatioan of a Novel Function of an Ubiquitin-editing Enzyme A20 by a Proteomic Analysis (2012)
  • Author(s): 山口 憲孝
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] Isolation of Lys63-linked Polyubiquitinated Proteins Using the Affinity Column Conjugated with the NZF Peptide Derived TAB2 (2012)
  • Author(s): 小嶋 絢
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] RANK Harbors the Specific Domain that is Crucial for Sspatiotemporal Regulation of RANK-mediated osteoclastogenic Signaling (2012)
  • Author(s): 田口 祐
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] Roles of Spatial Parameters for the Regulation of Nuclear Nf-κB Oscillation:Simulation on the 3D Spherical Cell Model (2012)
  • Author(s): 大島 大輔
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-11
• [Presentation] p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO (2012)
  • Author(s): 井上 純一郎
  • Organizer: 2nd Japanese-French Cancer Workshop
  • Place of Presentation: グランドエクシブ鳴門
  • Year and Date: 2012-11-29
  • Invited
• [Presentation] カラムスイッチHPLC法を用いたk63型ポリユビキチン化タンパク質の部分精製 (2012)
  • Author(s): 小嶋 絢
  • Organizer: 第49回ペプチド討論会
  • Place of Presentation: 鹿児島県民交流センター
  • Year and Date: 2012-11-09
• [Presentation] 転写因子NF-kappaB活性化を介した細胞間相互作用によるBasal-like乳癌幹細胞維持機構の解析 (2012)
  • Author(s): 山本 瑞生
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2012-09-19
• [Presentation] TRAF6はRasによるトランスフォームに必須ではない (2012)
  • Author(s): 山口 憲孝
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2012-09-19
• [Presentation] 乳がん悪性化における転写因子NF-kBの役割 (2012)
  • Author(s): 井上 純一郎
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2012-09-19
  • Invited
• [Presentation] 核と細胞質の間で振動する転写因子NF-kBの生物学 (2012)
  • Author(s): 井上 純一郎
  • Organizer: 日本応用数理学会２０１２年度年会
  • Place of Presentation: 稚内全日空ホテル
  • Year and Date: 2012-08-28
• [Presentation] 転写因子NF-kB振動の数理モデル (2012)
  • Author(s): 大島 大輔
  • Organizer: 日本応用数理学会２０１２年度年会
  • Place of Presentation: 稚内全日空ホテル
  • Year and Date: 2012-08-28
• [Presentation] Epigenetic Alteration of the NIK Gene is Involved in Enhanced NIK Expression in Basal-like Breast Cancer (2011)
  • Author(s): Jun-Ichiro Inoue Ph.D.
  • Organizer: Cincinnati Cancer Symposium Series
  • Place of Presentation: Cincinnati, Ohio The Westin Hotel USA
• [Presentation] Identification of a novel inhibitory factor in NF-kB activation pathway (2011)
  • Author(s): Yuri Shibata
  • Organizer: TNF2011 13th International TNF Conference
  • Place of Presentation: 淡路夢舞台
• [Presentation] A unique cytoplasmic domain in RANK induces long-term signaling required for osteoclastogenesis (2011)
  • Author(s): Yuu Taguchi
  • Organizer: TNF2011 13th International TNF Conference
  • Place of Presentation: 淡路夢舞台
• [Presentation] Lymphotoxin signal promotes thymic organogenesis by eliciting RANK expression in the embryonic thymic stroma (2011)
  • Author(s): Yasuhiro Mouri
  • Organizer: TNF2011 13th International TNF Conference
  • Place of Presentation: 淡路夢舞台
• [Presentation] Roles of TNF receptor family members on development of thymic microenvironment required for immunological self-tolerance (2011)
  • Author(s): Taishin Akiyama
  • Organizer: TNF2011 13th International TNF Conference
  • Place of Presentation: 淡路夢舞台
• [Presentation] RANK由来破骨細胞分化抑制ペプチドの同定とその作用機構の解明 (2011)
  • Author(s): 田口祐
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場
• [Presentation] 転写因子NF-kBによる乳癌細胞の増殖・維持機構の解析 (2011)
  • Author(s): 山本瑞生
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 悪性乳癌における、造腫瘍能促進に関与する新規NF-kB標的遺伝子の解析 (2011)
  • Author(s): 呉羽拓
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 破骨前駆細胞のRANKシグナルにおけるclAPタンパク質の機能解析 (2011)
  • Author(s): 山口憲孝
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Heregulin-B1/ErbB受容体シグナルはNF-kBを介して乳癌幹細胞の自己複製能を制御する (2011)
  • Author(s): 日野原邦彦
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Identification of p47 as a negative regulator of NF-kB (2011)
  • Author(s): Jun-ichiro Inoue
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Functional analysis of clAPs in RANK signaling in osteoclast precursor cells (2011)
  • Author(s): Noritaka Yamaguchi
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Oscillation of non-canonical NF-kB activation pathway (2011)
  • Author(s): Takao Seki
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Screening of novel NF-kB target genes in breast cancer cells (2011)
  • Author(s): Taku Ito-Kureha
  • Organizer: KEYSTONE SYMPOSIA "Stem Cells, Cancer and Metastasis"
  • Place of Presentation: アメリカ コロラド州Keystone Resort
• [Presentation] Paracrine role oftranscription factor NF-kB in generating breast cancer tumor initiating cells (2011)
  • Author(s): Mizuki Yamamoto
  • Organizer: KEYSTONE SYMPOSIA "Stem Cells, Cancer and Metastasis"
  • Place of Presentation: アメリカ コロラド州Keystone Resort
• [Presentation] 乳癌における新規NF-kB標的遺伝子の探索 (2010)
  • Author(s): 伊藤拓
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
• [Presentation] 乳癌源細胞における転写因子NF-kB活性化の役割 (2010)
  • Author(s): 山本瑞生
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
• [Presentation] Differential regulation of Lywsine 63-linked polyubiquitination catalyzed TRAF6 in IL-1 signaling (2010)
  • Author(s): J.Gohda
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2010-08-26
• [Presentation] ポリユビキチン化を介した転写因子NF-κBの活性制御機構と疾患発症への関与
  • Author(s): 井上 純一郎
  • Organizer: 日本生化学会関東支部例会
  • Place of Presentation: 山梨大学
• [Presentation] 転写因子NF-kBの活性制御機構とその破綻によるがんの悪性化
  • Author(s): 井上 純一郎
  • Organizer: 愛媛大学プロテオサイエンスセンター 第1回学術シンポジウム
  • Place of Presentation: 愛媛大学
  • Invited
• [Presentation] ショットガンプロテオミクスが解き明かす高解像度シグナル伝達ダイナミクス
  • Author(s): 尾山 大明
  • Organizer: 第86回 日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Book] Protein Modifications in Pathogenic Dysregulation of Signaling (2015)
  • Author(s): Jun-ichiro Inoue・Mutsuhiro Takekawa
  • Total Pages: 343
  • Publisher: Springer
• [Remarks] 「新学術領域研究」修飾シグナル病
  • URL: http://shushoku-signal.com/
• [Remarks] 新学術領域研究「修飾シグナル病」
  • URL: http://shushoku-signal.com/index.html
• [Remarks] 東京大学医科学研究所 癌細胞増殖部門 分子発癌分野
  • URL: http://www.traf6.com
• [Remarks] 新学術領域研究「修飾シグナル病」
  • URL: http://shushoku-signal.com/
• [Remarks] 東京大学医科学研究所 分子発癌分野
  • URL: http://www.ims.u-tokyo.ac.jp/BunshiHatsugan/index.html
• [Remarks]
  • URL: http://shushoku-signal.com/
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/BunshiHatsugan/index.html
• [Remarks]
  • URL: http://shushoku-signal.com/
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/BunshiHatsugan/index.html