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Searching for signaling molecules in neuro-vascular interaction

Planned Research

Project AreaVasculo-neural wiring and their interdependent crosstalk
Project/Area Number 22122008
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo (2013-2015)
Osaka Bioscience Institute (2010-2012)

Principal Investigator

Emoto Kazuo  東京大学, 理学(系)研究科(研究院), 教授 (80300953)

Co-Investigator(Kenkyū-buntansha) Koizumi Hiroyuki  東京大学, 大学院理学系研究科, 助教 (10334335)
Kanai Makoto  大阪バイオサイエンス研究所, 神経細胞生物学部門, 研究員 (50598034)
Kanamori Rei (Morikawa Rei)  大阪バイオサイエンス研究所, 神経細胞生物学部門, 研究員 (50534575)
Project Period (FY) 2010-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥163,930,000 (Direct Cost: ¥126,100,000、Indirect Cost: ¥37,830,000)
Fiscal Year 2014: ¥27,950,000 (Direct Cost: ¥21,500,000、Indirect Cost: ¥6,450,000)
Fiscal Year 2013: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2012: ¥29,380,000 (Direct Cost: ¥22,600,000、Indirect Cost: ¥6,780,000)
Fiscal Year 2011: ¥32,500,000 (Direct Cost: ¥25,000,000、Indirect Cost: ¥7,500,000)
Fiscal Year 2010: ¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000)
Keywordsショウジョウバエ / 末梢感覚ニューロン / 気管 / 伴走構造 / NCAM / 軸索 / 感覚ニューロン / 接着因子 / 並走構造 / IgGスーパーファミリー / RNAi / スクリーニング / 樹状突起 / ライブイメージング
Outline of Final Research Achievements

To identify molecules involved in neuro-vascular interaction, we screened molecules involved in neuro-trachea interaction in Drosophila as a model for neuro-vascular interaction. We conducted an RNAi screen for ~100 candidate molecules and found that FasII/NCAM, an adhesion molecule of IgG superfamily, mediates interaction between trachea and axons of sensory neurons in wing disc, which likely navigates afferent axons to the central nervous system.

Report

(6 results)
  • 2015 Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (20 results)

All 2015 2013 2012 2011 2010

All Journal Article (12 results) (of which Peer Reviewed: 11 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (2 results)

  • [Journal Article] Adult Drosophila sensory neurons specify dendrite boundaries independent of dendritic contact through the Wnt5-Drl signaling pathway.2015

    • Author(s)
      Yasunaga K, Tezuka A, Ishikawa N, Dairyo Y, Togashi K, Koizumi H and Emoto K
    • Journal Title

      Genes and Development

      Volume: 29 Issue: 16 Pages: 1763-1775

    • DOI

      10.1101/gad.262592.115

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Local endocytosis triggers dendritic thinning and pruning in Drosophila sensory neurons2015

    • Author(s)
      Takahiro Kanamori, Jiro Yoshino, Kei-ichiro Yasunaga, Yusuke Dairyo, Kazuo Emoto
    • Journal Title

      Nature Communications

      Volume: 6 Issue: 1 Pages: 6515-6515

    • DOI

      10.1038/ncomms7515

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Role for phospholipid flippase complex of ATP8A1 and CDC50A in cell migration.2013

    • Author(s)
      Kato, U., Inadome, H., Emoto, K., Kobayashi, T., Umeda, M.
    • Journal Title

      J Biol Chem

      Volume: 288 Issue: 7 Pages: 4922-4934

    • DOI

      10.1074/jbc.m112.402701

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Signaling mechanisms that coordinate the development and maintenance of dendritic fields.2012

    • Author(s)
      Emoto, K.
    • Journal Title

      Curr. Opin. Neurobiol.

      Volume: 22 Issue: 5 Pages: 805-811

    • DOI

      10.1016/j.conb.2012.04.005

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salt-inducible kinase 1 regulates E-cadherin expression and intercellular junction stability.2012

    • Author(s)
      Eneling, K.
    • Journal Title

      FASEB J.

      Volume: 26 Issue: 8 Pages: 3230-3239

    • DOI

      10.1096/fj.12-205609

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis2012

    • Author(s)
      Lee, S., Uchida1, Y., Emoto, K., Umeda, M., Kuge, O., Taguchi, T., Arai, H
    • Journal Title

      Genes to Cells

      Volume: 17 Issue: 8 Pages: 728

    • DOI

      10.1111/j.1365-2443.2012.01622.x

    • URL

      https://localhost/en/publications/5c331496-6379-4a3f-9464-44bbabc21a48

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The growing role of the Hippo-NDR kinase signaling in neuronal development and disease2011

    • Author(s)
      Emoto, K.
    • Journal Title

      J.Biochem.

      Volume: 150 Issue: 2 Pages: 133-141

    • DOI

      10.1093/jb/mvr080

    • NAID

      10030586649

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dendrite remodeling in development and disease2011

    • Author(s)
      Emoto, K.
    • Journal Title

      Dev.Growth Differ.

      Volume: 53 Issue: 3 Pages: 277-286

    • DOI

      10.1111/j.1440-169x.2010.01242.x

    • NAID

      10028202226

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Different levels of the tripartite motif protein Asap regulate distinct axonal projections of Drosophila sensory neurons2011

    • Author(s)
      Morikawa, et al
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 108 Issue: 48 Pages: 19389-19394

    • DOI

      10.1073/pnas.1109843108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dendrite reshaping of adult Drosophila sensory neurons requires matrix metalloproteinase-mediated modification of the basement membranes.2010

    • Author(s)
      Yasunaga, et al.
    • Journal Title

      Developmental Cell

      Volume: 18 Pages: 621-632

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Drosophila Furry protein interacts with Trc and is highly mobile in vivo.2010

    • Author(s)
      Fang, et al.
    • Journal Title

      BMC Developmental Biology

      Volume: 10 Pages: 40-40

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 神経ネットワーク再編の鍵を握る細胞外マトリックス分解機構2010

    • Author(s)
      榎本和生
    • Journal Title

      生化学

      Volume: 82 Pages: 972-978

    • NAID

      10027502887

    • Related Report
      2010 Annual Research Report
  • [Presentation] Local calcium signaling in dendrite remodeling.2015

    • Author(s)
      Kazuo Emoto
    • Organizer
      Gordon Research Conference “Dendrite: Molecules, Structure & Function”
    • Place of Presentation
      Ventura Beach, USA
    • Year and Date
      2015-04-16
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Instructive cues from tracheal tubes are required for proper development of afferent axons in Drosophila.2013

    • Author(s)
      Taiki Hashimoto, Makoto Kanai, Kazuo Emoto
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      ウインクあいち
    • Year and Date
      2013-06-19 – 2013-06-21
    • Related Report
      2013 Annual Research Report
  • [Presentation] 感覚ニューロンの受容領域を規定する分子細胞基盤2013

    • Author(s)
      榎本和生
    • Organizer
      大阪大学蛋白質研究所神経科学セミナー
    • Place of Presentation
      大阪大学蛋白質研究所
    • Year and Date
      2013-03-17
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Molecular and cellular basis for dendrite development2012

    • Author(s)
      Kazuo Emoto
    • Organizer
      EMBOカンファレンス
    • Place of Presentation
      ギリシアクレタ島
    • Year and Date
      2012-06-15
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Molecular and cellular basis for dendrite development2011

    • Author(s)
      Emoto, K.
    • Organizer
      Cold Spring Harbor Meeting Asia
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2011-10-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Control of dendritic fields in sensory neurons by TOR signaling2010

    • Author(s)
      Kazuo Emoto
    • Organizer
      日本生化学会分子生物学会合同大会(BMB2010)
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Book] 神経ー血管ワイヤリング研究のための古くて新しいモデル生物ショウジョウバエ2013

    • Author(s)
      金井誠、橋本大輝、木下裕介、榎本和生
    • Total Pages
      6
    • Publisher
      メディカルレビュー社
    • Related Report
      2013 Annual Research Report
  • [Book] Hippo pathwayの多彩な細胞機能2011

    • Author(s)
      藤岡洋美・泉あやか・榎本和生
    • Total Pages
      8
    • Publisher
      学研バイオメディカル秀潤社(細胞工学)
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2018-03-28  

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