|Budget Amount *help
¥102,700,000 (Direct Cost: ¥79,000,000、Indirect Cost: ¥23,700,000)
Fiscal Year 2014: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2012: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2010: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
|Outline of Final Research Achievements
We found that Tsukushi is expressed in the subventricular zone (SVZ) in the lateral ventricles (LV). In the Tsukushi KO mouse brain, both the cell proliferation and the cell death in the SVZ were increase compared to those of the wild type brain.
To examine the involvement of Tsukushi protein for niche regulation, we generated the transgenic mice that express the Tsukushi protein. When Tsukushi protein was produced at the SVZ, the expansion of LV was rescued. We used cre-loxp system to specifically delete Tsukushi gene in the SVZ area. When we use SM22-cre mouse, the LV expansion was observed but not with Tie2-cre mouse. We also examined the deletion of Tsukushi gene in the nervous system and found that both the nestin-cre and sox2-cre mice did not show the LV expansion. We are now preparing FOXJ1-cre mouse, which is specifically expressed ependymal cells. Our data suggested that Tsukushi functions as a niche molecule for the regulation of neural stem/progenitor cells.