| Budget Amount *help |
¥83,460,000 (Direct Cost : ¥64,200,000、Indirect Cost : ¥19,260,000)
Fiscal Year 2015 : ¥12,740,000 (Direct Cost : ¥9,800,000、Indirect Cost : ¥2,940,000)
Fiscal Year 2014 : ¥14,560,000 (Direct Cost : ¥11,200,000、Indirect Cost : ¥3,360,000)
Fiscal Year 2013 : ¥15,990,000 (Direct Cost : ¥12,300,000、Indirect Cost : ¥3,690,000)
Fiscal Year 2012 : ¥19,110,000 (Direct Cost : ¥14,700,000、Indirect Cost : ¥4,410,000)
Fiscal Year 2011 : ¥21,060,000 (Direct Cost : ¥16,200,000、Indirect Cost : ¥4,860,000)
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| Outline of Final Research Achievements |
In this study, we tried to elucidate how to design artificial polycistronic operon, especially in terms of gene order in the operon. We used carotenoid and anthocyanin biosynthetic pathway which don't exist in Escherichia coli.
As a result, we succeeded in construction of artificial carotenoid operon plasmid that is fully responsible for carotenoid production starting from glucose. We also succeeded in construction of artificial anthocyanin operon that is designed using information of optimal ration of responsible 4 enzymes by in vitro experiment. Through these case studies, we propose possible rule for construction of artificial operon.
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