Immunohistochemical Study of Atrial and Brain Natriuretic Polypeptide
| Project/Area Number | 01570649 |
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Miyazaki Medical College, Internal Medicine |
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Principal Investigator |
TOSHIMORI Hirotaka Miyazaki Medical College, Internal Medicine Assistant., 医学部, 助手 (60172160)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed(Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1990 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1989 : ¥1,300,000 (Direct Cost : ¥1,300,000)
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| Keywords | Atrial Natriuretic Polypeptide / Ventricular Cardiocyte / Impulse Conduction / Spontaneously Hypertensive rat Stroke Prone / Hypertension / Immunohistochemistry / atrial natriuretic polypeptide / ventricular cardiocyte / impulse conduction / hypertension / Spontaneously Hypertensive rat Stroke Prone / immunohistochemistry / atrial cardiocyte / atrial specific granule / spontaneously hypertensive rat stroke-prone / rat / chicken |
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| Research Abstract |
Ventricular cardiocytes were immunohistochemically examined using antisera against Atrial Natriuretic Polypeptide (ANP) and Brain Natriuretic Polypeptide (BNP). The cells with ANP-containing specific ganules were distributed in both the atrial ventricular walls, which was corresponded with the results revealed a presence of ANPmRNA by Northern blotting analysis of rat heart tissues. Strands of Purkinje fibers were clearly demonstrated in the subendocardial region by the immunohistochemical staining for ANP in the rat ventricle in spite of difficult visualization of them by conventional HE or PAS stainings. Purkinje type III fibers (transitional cells) could be identifid in porcine hearts. They were, however, hard to demonstrated in the human ventricle. Purkinje fibers were also immunohistochemically stained in Avean hearts using antiserum against chicken-ANP. Impulse conducting system stained for ANP were not found in other vertebrates in spite of a presence of ANP -immunoreactive vent
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ricular cardiocytes.
ANP- immunoreactive cells were found in the periarterial regions of the ventricular wall in rat and chicken hearts. These cells were morphologically differed from those of periarterial Purkinje fibers characteristic in Avian hearts. They did not connect with any cells belong to an impulse conducting system. These periarterial ANP-cells were also seen in newborn rat hearts. Some local endocrinal interaction between these ANP-cells and arteries was suggested. Marked increasing of ANPmRNA had been well known in the ventricular tissue of Spontaneous Hypertensive Rat stroke Prone (SHRP). The ANP-immunoreactivities were chiefly spread in a whole of hypertrophied left ventricular wall including the left side of the interventricular septum, and it was suggested that these ANP-cells responded to hypertensive state or heart failure. Secretional form of the peptide from the cells was considered to be constitutive secretion by means of immunoelectron microscopy for ANP.
Amyloid-like deposits immunoreactive for both ANP and BNP were found in the cytoplasm of human ventricular cells suggesting a disturbance of the peptide secretion. This phenomenon is remained to be examined from a standpoint of an aging of the endocrine cardiac cells. Less
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Report
(3results)
Research Output
(22results)
