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Immunohistochemical Study of Atrial and Brain Natriuretic Polypeptide

Research Project

Project/Area Number01570649
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionMiyazaki Medical College, Internal Medicine

Principal Investigator

TOSHIMORI Hirotaka  Miyazaki Medical College, Internal Medicine Assistant., 医学部, 助手 (60172160)

Project Period (FY) 1989 – 1990
Project Status Completed(Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1990 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1989 : ¥1,300,000 (Direct Cost : ¥1,300,000)
KeywordsAtrial Natriuretic Polypeptide / Ventricular Cardiocyte / Impulse Conduction / Spontaneously Hypertensive rat Stroke Prone / Hypertension / Immunohistochemistry / atrial natriuretic polypeptide / ventricular cardiocyte / impulse conduction / hypertension / Spontaneously Hypertensive rat Stroke Prone / immunohistochemistry / atrial cardiocyte / atrial specific granule / spontaneously hypertensive rat stroke-prone / rat / chicken
Research Abstract

Ventricular cardiocytes were immunohistochemically examined using antisera against Atrial Natriuretic Polypeptide (ANP) and Brain Natriuretic Polypeptide (BNP). The cells with ANP-containing specific ganules were distributed in both the atrial ventricular walls, which was corresponded with the results revealed a presence of ANPmRNA by Northern blotting analysis of rat heart tissues. Strands of Purkinje fibers were clearly demonstrated in the subendocardial region by the immunohistochemical staining for ANP in the rat ventricle in spite of difficult visualization of them by conventional HE or PAS stainings. Purkinje type III fibers (transitional cells) could be identifid in porcine hearts. They were, however, hard to demonstrated in the human ventricle. Purkinje fibers were also immunohistochemically stained in Avean hearts using antiserum against chicken-ANP. Impulse conducting system stained for ANP were not found in other vertebrates in spite of a presence of ANP -immunoreactive vent … More ricular cardiocytes.
ANP- immunoreactive cells were found in the periarterial regions of the ventricular wall in rat and chicken hearts. These cells were morphologically differed from those of periarterial Purkinje fibers characteristic in Avian hearts. They did not connect with any cells belong to an impulse conducting system. These periarterial ANP-cells were also seen in newborn rat hearts. Some local endocrinal interaction between these ANP-cells and arteries was suggested. Marked increasing of ANPmRNA had been well known in the ventricular tissue of Spontaneous Hypertensive Rat stroke Prone (SHRP). The ANP-immunoreactivities were chiefly spread in a whole of hypertrophied left ventricular wall including the left side of the interventricular septum, and it was suggested that these ANP-cells responded to hypertensive state or heart failure. Secretional form of the peptide from the cells was considered to be constitutive secretion by means of immunoelectron microscopy for ANP.
Amyloid-like deposits immunoreactive for both ANP and BNP were found in the cytoplasm of human ventricular cells suggesting a disturbance of the peptide secretion. This phenomenon is remained to be examined from a standpoint of an aging of the endocrine cardiac cells. Less

Report

(3results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report

Research Products

(22results)

All Other

All Publications

  • [Publications] Toshimori et al.: "Chicken atrial natriuretic polypeptide(chANP)and its secretion." Cell Tissue Res. 259. 293-298 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Atrial natriuretic polypeptide(ANP)ーimmunoreactive cells in the perivascular region of the ventricular walls of human,rat and chicken hearts." in preparation(1991).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Amyloid deposition commonly immnoreactive for atrial natriuretic polypeptide(ANP)and brain natriuretic polypeptide(BNP)in human ventricular wall of the heart." in preparation(1991).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Distribution Atrial natriuretic polypeptide(ANP)ーcontaining cells in the heart and lung of rats." Proceeding of symposium of ANP in KYOTO,Kodansha,Tokyo,in press(1991).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Islet amyloid polypeptide(IAPP)in the gastrointestinal tract and pancreas in man and rats." Celltissue Res,. 262. 401-406 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Islet amyloid polypeptide in insulinoma and in the islets of the pancreas of nonーdiabetic and diabetic subjects." Virchow Arch A.Path Anat and Histol,in press.(1991).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Chicken atrial natriuretic polypeptide (chANP) and its secretion." Cell Tissue Res. 259. 293-298 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Atrial natriuretic polypeptide (ANP)-immunoreactive cells in the perivascular region of the ventricular walls of human, rat and chicken hearts." (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al: "Amyloid deposition commonly immunoreactive for atrial natriuretic polypeptide (ANP) and brain natriuretic polypeptide (BNP) in human ventricular wall of the hearts." (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Distribution of atrial natriuretic polypeptide (ANP)- containing cells in the heart and lung of rats." Proceeding of symposium of ANP in Kyoto. Kodansha, Tokyo. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Islet amyloid polypeptide (IAPP) in the gastrointestinal tract and pancreas in man and rats." Cell Tissue Res. 262. 401-406 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Islet amyloid polypeptide in insulinoma and in the islets of the pancreas of non-diabetic and diabetic subjects." Virchows Archiv. A Pathol. Anat.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshimori et al.: "Chicken atrial natriuretic polypeptide (chANP) and its secretion." Cell Tissue Res. 259. 293-298 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshimori et al.: "Atrial natriuretic polypeptide (ANP)ーimmunoreactive cells in the perivascular region of the ventricular walls of human,rat and chicken hearts." (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshimori et al.: "Amyloid deposition commonly immnoreactive for atrial natriuretic polypeptide (ANP) and brain natriuretic polypeptide (BNP) in human ventricular wall of the heart." (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshimori et al.: "Distribution Atrial natriuretic polypeptide (ANP)ーcontaining cells in the heart and lung of rats." Proceeding of symposium of ANP in KYOTO,Kodansha,Tokyo,. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshimori et al.: "Islet amyloid polypeptide (IAPP) in the gastrointestinal tract and pancreas in man and rats." Cell Tissue Res,. 262. 401-406 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshimori et al.: "Islet amyloid polypeptide in insulinoma and in the islets of the pancreas of nonーdiabetic and diabetic subjects." Virchow Arch A.Path Anat and Histol,. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Toshimori,K.Toshimori,N.Minamino,K.Kangawa,C.Oura,S.Matsukura,H.Matsuo: "Chicken atrial natriuretic peptide(chANP)and its secretion." Cell and Tissue Research. 259. 293-298 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Toshimori,R.Narita,K.Kangawa,H.Matsuo,S.Matsukura: "Atrial natriuretic polypeptide(ANP)-immunoreactive cells in the perivascular region of the ventricular wall of normal and spontaneously hypertension stroke-prone(SHRSP)rats and chiken."

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Toshimori,R.Narita,M.Nakazato,J.Asai,T.Mitsukawa,K.Kangawa,H.Matsuo,S.Matsukura: "Islet amyloid polypeptide(IAPP)in the gastrointestinal tract and pancreas in man and rats." Cell and Tissue Research.

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Toshimori,R.Narita,M.Nakazato,J.Asai,T.Mitsukawa,K.Kangawa,H.Matsuo,S.Matsukura: "Islet amyloid polypeptide(IAPP)in insulinoma and islets of the pancreas of non-diabetic and diabetic subjects." Virchows Archiv A.Pathological anatomy and Histology.

    • Related Report
      1989 Annual Research Report

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Published : 1989-04-01   Modified : 2016-04-21  

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