|Budget Amount *help
¥2,400,000 (Direct Cost : ¥2,400,000)
Fiscal Year 1992 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1991 : ¥900,000 (Direct Cost : ¥900,000)
Fiscal Year 1990 : ¥800,000 (Direct Cost : ¥800,000)
A New assay method for the screening of anti-emetic activity using frog, and the usefulness of this method were established. By using this method, the anti-emetic activities of 50 Chinese cude drug extracts were compared with the activities of known drugs, chlorpromazine, metoclopramide and ethyl-p-amino-benzoate. As the results, 18 compounds, , , -shogaol, , , -gingerol, magnolol, honokiol, beta-eudesmol, cryptomeridiol, rutin, phillyrin, rengyol, taraxasteryl palmitate, taraxasteryl acetate, IF-3, IF-4 and chlorogenic acid, were isolated as anti-emetic principlesby using modern chromatographical techniques, and the structures were identified by a variety of spectraldata.